FastQCFastQC Report
Thu 11 Sep 2014
LNvs_CT0_1_ATCACG_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLNvs_CT0_1_ATCACG_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19678674
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA1289250.65515085010301No Hit
GCATGAGTTGTTAATTTGGGCACCGTAACATTACGTTTGGTTCATCCCAC262890.13359131819552475No Hit
GCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT258330.13127408889440417No Hit
CATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAA256730.1304610259817303No Hit
CCACTTCAGTCCTCCAAGGTCTCATTCGATTATTTGCTACTACCACCAAG252170.12814379668060968No Hit
CATTCGATTATTTGCTACTACCACCAAGATCTGTACCAATGGCAGCTCCA241900.12292494910988412No Hit
TTCGCATTGTTAATCATACAAGTGCATATAATAAACACAAAAATCAATGA228910.11632389458761297No Hit
AGTGGTTGTCTGTACTGCCGTCTGGCTGGCTCTCGGGTCAGTTTCAGGCG228740.11623750665314136No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA225990.1148400547719831No Hit
CGTGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA225090.11438270688360404No Hit
GGTGGTGACTCTGAATAACTTTCTAGCTGATCGCATAGCCTTGCGCTGGC220770.11218743701938454No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG200800.10203939554057351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA126500.057.659467
GATCTAG131950.055.889728
GGTTATA357950.052.8034634
GTCGATC126250.052.1989445
TCGATCT137900.050.3027536
GTTATAT380500.050.261845
TGGTTAT375800.049.0193443
GGTCGAT139700.045.234964
TTATATT452450.043.0143286
CATGGTT301300.042.7633061
CGTGGTT76100.042.483771
CGCTAGT27700.042.1645934
TATATTT512900.039.9130747
TAACGTT298350.036.61145894-95
TAATACG46650.035.5257848
TATTTAG648800.035.511669
ATGGTTA418750.035.4289252
TTAACGT316700.034.45263794-95
CTTAATC313000.033.9687547
GTGGTTA86950.033.2514422
Produced by FastQC (version 0.11.2)