FastQCFastQC Report
Thu 11 Sep 2014
LNvs_CT0_1_ATCACG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLNvs_CT0_1_ATCACG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19678674
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTG432990.22003006909916797No Hit
GGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGT229360.11655256853180249No Hit
GGGGGTTCGAAGGCGATCAGATACCGCCCTAGTTCTAACCATAAACGATG218700.11113553687611269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGT202250.062.6607551
GGGGTAT119000.056.244461
GGGGGTT224950.053.3157161
GCGCTCT140400.052.6777953
GGGGTAC82700.051.1574061
GGGGACG161000.049.6029431
GGGGGGA336750.048.9971731
CGGGTAT43300.048.3046341
GGGGGAC229350.047.836691
GGGGGAG280800.045.132261
GGGCGCT157450.045.0151821
GATAATT143150.044.166483
GGGGGTA161550.042.666311
GGGGTGG190150.042.3237951
GGGGGGG365750.042.1359441
GGGGTAG146450.041.969441
CCGCTCT84300.040.7396053
CGGGGAC75950.040.5575071
GGGGGAT258400.040.4903531
GGGGGTC150300.040.3883361
Produced by FastQC (version 0.11.2)