FastQCFastQC Report
Tue 12 Aug 2014
lane8_Undetermined_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane8_Undetermined_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4083740
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 967815 3.6322172 3.8461134 36
TTTGA 590370 2.1263752 7.796047 8
TGTTT 584395 1.9523175 7.2943983 6
CTTTG 402255 1.7782716 8.9828 1
GTTTG 408375 1.7689915 8.639306 7
TTGAG 328750 1.5353379 6.052826 9
TGTGT 323475 1.4012231 8.073517 4
GTGTT 310835 1.3464695 8.267784 5
TTTGT 396780 1.3255428 6.6411653 2
AACTT 302200 1.1975998 5.3452077 2
TTGTG 242675 1.0512152 7.978188 3