##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane8_Undetermined_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4083740	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.842349414997038	34.0	31.0	34.0	31.0	34.0
2	31.946200541660343	34.0	31.0	34.0	31.0	34.0
3	32.00622762467738	34.0	31.0	34.0	31.0	34.0
4	35.32277520116364	37.0	37.0	37.0	35.0	37.0
5	35.334633693623985	37.0	37.0	37.0	35.0	37.0
6	35.346093287035906	37.0	37.0	37.0	35.0	37.0
7	35.31889468967172	37.0	37.0	37.0	35.0	37.0
8	35.34541008977065	37.0	37.0	37.0	35.0	37.0
9	37.07791019996376	39.0	38.0	39.0	35.0	39.0
10	37.043637205111004	39.0	38.0	39.0	35.0	39.0
11	37.01893754254678	39.0	38.0	39.0	35.0	39.0
12	36.98213892167474	39.0	38.0	39.0	35.0	39.0
13	36.95864207809508	39.0	38.0	39.0	35.0	39.0
14	38.46085255182749	41.0	40.0	41.0	36.0	41.0
15	38.43506075313316	41.0	39.0	41.0	36.0	41.0
16	38.421411989989565	41.0	39.0	41.0	36.0	41.0
17	38.416450361678265	41.0	39.0	41.0	36.0	41.0
18	38.399137555280205	41.0	39.0	41.0	35.0	41.0
19	38.423314902515834	41.0	39.0	41.0	35.0	41.0
20	38.383819733871405	41.0	39.0	41.0	35.0	41.0
21	38.34512921978383	41.0	39.0	41.0	35.0	41.0
22	38.29902075058647	41.0	39.0	41.0	35.0	41.0
23	38.296352853021986	41.0	39.0	41.0	34.0	41.0
24	38.258449117720524	41.0	39.0	41.0	34.0	41.0
25	38.20567372065803	41.0	39.0	41.0	34.0	41.0
26	38.148081415565144	40.0	39.0	41.0	34.0	41.0
27	38.10616518191657	40.0	39.0	41.0	34.0	41.0
28	38.07728111975787	40.0	39.0	41.0	34.0	41.0
29	38.01876857978226	40.0	38.0	41.0	34.0	41.0
30	37.98524563267985	40.0	38.0	41.0	34.0	41.0
31	37.954080328326484	40.0	38.0	41.0	33.0	41.0
32	37.888124121516064	40.0	38.0	41.0	33.0	41.0
33	37.77102068202187	40.0	38.0	41.0	33.0	41.0
34	37.689237071899775	40.0	38.0	41.0	33.0	41.0
35	37.636684999534744	40.0	38.0	41.0	33.0	41.0
36	37.58139915861441	40.0	38.0	41.0	33.0	41.0
37	37.522322674803	40.0	38.0	41.0	33.0	41.0
38	37.433044709016734	40.0	38.0	41.0	32.0	41.0
39	37.371952425962476	40.0	38.0	41.0	32.0	41.0
40	37.288492166494436	40.0	38.0	41.0	32.0	41.0
41	37.47181701087728	40.0	38.0	41.0	33.0	41.0
42	37.50037416681767	40.0	38.0	41.0	33.0	41.0
43	37.47000519131972	40.0	38.0	41.0	33.0	41.0
44	37.42632366409223	40.0	38.0	41.0	32.0	41.0
45	37.37241229853027	40.0	38.0	41.0	32.0	41.0
46	37.304739283108134	40.0	38.0	41.0	32.0	41.0
47	37.224948698986715	40.0	38.0	41.0	32.0	41.0
48	37.11994274855892	40.0	37.0	41.0	32.0	41.0
49	37.04042128049288	40.0	37.0	41.0	31.0	41.0
50	36.92625730335428	40.0	37.0	41.0	31.0	41.0
51	35.5271662740527	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1780.0
3	1051.0
4	2523.0
5	1995.0
6	2714.0
7	7547.0
8	21852.0
9	27514.0
10	25023.0
11	15890.0
12	10351.0
13	9194.0
14	8142.0
15	7855.0
16	7799.0
17	7341.0
18	7169.0
19	6632.0
20	6744.0
21	6976.0
22	7438.0
23	8067.0
24	8615.0
25	9272.0
26	10251.0
27	11555.0
28	13372.0
29	16037.0
30	19146.0
31	23986.0
32	31950.0
33	43469.0
34	63647.0
35	99085.0
36	166011.0
37	328189.0
38	902398.0
39	2134869.0
40	291.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.515679500532524	29.101244089162215	28.949091061370837	21.433985348934424
2	22.536348989500052	29.868402741707552	28.313402967112445	19.281845301679954
3	21.908843001674317	28.07279171217149	28.793803780333043	21.224561505821146
4	21.90679986224528	26.17739229333422	29.802841620064147	22.11296622435636
5	23.453847873930492	26.654108164938584	29.57679173188653	20.31525222924439
6	22.165954309822325	26.21856962215179	29.74290100052362	21.872575067502265
7	23.27753145534347	26.469359244231622	29.55521202248911	20.6978972779358
8	22.467466459761535	26.673525309866626	29.629805142509184	21.229203087862658
9	22.19756488706038	26.410435427052747	30.036969642684046	21.355030043202824
10	22.39804645085007	27.00219499076227	29.388894226410116	21.21086433197755
11	22.914278730301906	26.566189108083787	29.26783504125346	21.251697120360845
12	22.062707476395698	27.21059464614027	29.330202904626486	21.39649497283755
13	22.38937022711446	26.58654327822389	29.510799155474803	21.513287339186853
14	22.63574699057927	26.891887301728733	29.04336529368589	21.429000414006108
15	22.247565895533576	27.203112857324662	28.868861752614293	21.680459494527465
16	22.509973101542293	26.67172807209673	29.087027462021002	21.731271364339978
17	22.567334344952204	26.8434640228907	28.972582572530076	21.61661905962702
18	22.323772816322276	26.606468634407047	28.99696650673492	22.07279204253575
19	22.28698028704221	26.639326738921138	29.151990438151458	21.92170253588519
20	22.364144848582274	26.793340107471852	29.026446177798032	21.816068866147837
21	22.196033300504965	26.61529703524193	29.081998374565515	22.10667128968759
22	22.2357913636348	26.71245268622295	29.11040512244921	21.941350827693043
23	22.44770456352572	26.72305334501523	28.949373014472187	21.879869076986864
24	22.192732108203238	26.737619717313933	28.992459478782834	22.0771886957
25	22.29376575970857	26.744733821425072	28.904039670539305	22.057460748327053
26	22.261091254639766	26.828702355222482	28.894471805981915	22.015734584155837
27	22.32870160315509	26.909882067599632	28.664465091449102	22.096951237796176
28	22.298658648694943	26.849014662149195	28.726214438533383	22.12611225062248
29	22.40308975975925	26.830765364435123	28.656162131398233	22.109982744407393
30	22.212742721751013	26.825642632044634	28.693335897540933	22.26827874866342
31	22.1021120619128	26.780013850310187	28.96167075070104	22.156203337075976
32	22.457300132180716	26.85393454171422	28.643342957555195	22.045422368549872
33	22.319000682944367	26.726168327956916	28.646282281540802	22.30854870755791
34	22.229886761561925	26.652197351736106	28.855816716319254	22.26209917038271
35	22.39214869276217	26.853808237637338	28.58350226656186	22.17054080303863
36	22.34583321945626	26.713345186397685	28.615006117353214	22.325815476792837
37	22.37614133398661	26.620174143837932	28.648224769394215	22.355459752781243
38	22.5418273088066	26.690526812611143	28.69176391412811	22.075881964454148
39	22.27970776286295	26.68215394117725	28.63972705094743	22.398411245012362
40	22.23760632171519	26.81429653672988	28.59726165766409	22.350835483890837
41	22.372584193853015	26.7632352995495	28.60220905364792	22.26197145294957
42	22.18464647638539	26.669253606984345	28.67204769950259	22.474052217127678
43	22.233356315876208	26.811365544857463	28.720074352097974	22.235203787168352
44	22.397111454618123	26.732736905122206	28.645275269539038	22.224876370720626
45	22.410266084966512	26.548279158935266	28.686480397174385	22.354974358923837
46	22.32006923227339	26.5928323713088	28.781977936892044	22.305120459525767
47	22.416041369245587	26.70461835575571	28.611060283933337	22.268279991065363
48	22.35759909596191	26.606129750751457	28.542608116387758	22.493663036898877
49	22.160095499415196	26.79167921693238	28.739989444231135	22.30823583942129
50	22.226800566816586	26.72697923726203	28.802562996734643	22.243657199186742
51	22.40422365614303	26.51153398954441	28.670847127671216	22.41339522664134
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.94966484946695
2	41.818194291180006
3	43.13340450749546
4	44.019766086601635
5	43.769100103174885
6	44.03852937732459
7	43.97542873327927
8	43.69666954762419
9	43.552594930263204
10	43.60891078282762
11	44.165975850662754
12	43.459202449233246
13	43.90265756630131
14	44.064747404585376
15	43.928025390061045
16	44.241244465882275
17	44.18395340457922
18	44.39656485885803
19	44.2086828229274
20	44.180213714730115
21	44.302704590192555
22	44.177142191327846
23	44.32757364051258
24	44.26992080390323
25	44.35122650803562
26	44.2768258387956
27	44.425652840951265
28	44.42477089931742
29	44.513072504166644
30	44.48102147041443
31	44.25831539898877
32	44.50272250073059
33	44.62754939050228
34	44.49198593194464
35	44.5626894958008
36	44.6716486962491
37	44.73160108676785
38	44.61770927326075
39	44.67811900787531
40	44.588441805606024
41	44.63455564680259
42	44.65869869351307
43	44.46856010304456
44	44.62198782533875
45	44.765240443890356
46	44.62518969179916
47	44.684321360310946
48	44.85126213286079
49	44.468331338836485
50	44.47045776600333
51	44.81761888278437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3079.0
1	2796.0
2	2513.0
3	2623.0
4	2733.0
5	3522.0
6	4311.0
7	4535.5
8	4760.0
9	4146.5
10	3533.0
11	3711.0
12	3889.0
13	4268.0
14	4647.0
15	5666.0
16	6685.0
17	8747.0
18	10809.0
19	13055.5
20	15302.0
21	17734.5
22	20167.0
23	23657.5
24	27148.0
25	34331.0
26	51269.0
27	61024.0
28	65812.5
29	70601.0
30	83966.0
31	97331.0
32	108186.5
33	119042.0
34	148752.5
35	178463.0
36	207344.5
37	236226.0
38	273992.0
39	311758.0
40	334451.5
41	357145.0
42	390836.0
43	424527.0
44	439172.0
45	453817.0
46	436009.0
47	418201.0
48	361173.0
49	304145.0
50	271978.5
51	239812.0
52	207825.5
53	175839.0
54	150776.5
55	125714.0
56	111734.5
57	97755.0
58	83117.5
59	68480.0
60	66604.5
61	64729.0
62	50838.5
63	36948.0
64	31587.0
65	26226.0
66	21519.0
67	16812.0
68	14190.5
69	11569.0
70	10211.0
71	8853.0
72	7631.5
73	6410.0
74	5449.5
75	3798.0
76	3107.0
77	2991.5
78	2876.0
79	2805.0
80	2734.0
81	2342.0
82	1950.0
83	1807.0
84	1664.0
85	1492.5
86	1321.0
87	1513.5
88	1706.0
89	1217.5
90	729.0
91	511.0
92	293.0
93	226.5
94	160.0
95	124.0
96	88.0
97	67.0
98	46.0
99	53.0
100	60.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008595062369298731
2	0.05377423636176642
3	0.06540573102107382
4	0.09856161263939428
5	0.12801990332391386
6	0.24910988456659827
7	0.31757653523485824
8	0.26985067609593166
9	0.2148765592324683
10	0.34328826027122195
11	0.4234598676703218
12	0.46822275659077217
13	0.5225357148104434
14	0.5141855260129194
15	0.523123411382703
16	0.4973382242748069
17	0.3676531806628238
18	0.3551646285023043
19	0.34855304206438215
20	0.37529323610220044
21	0.41959086523627853
22	0.38410868468609655
23	0.24979553056756798
24	0.26475730580301393
25	0.35318115257092764
26	0.3765420913182524
27	0.3706896129528325
28	0.2177905547365895
29	0.22330021010152457
30	0.1740316474604162
31	0.17868424532413915
32	0.20224108292888382
33	0.4290919598211444
34	0.5679352750175085
35	0.5623276702238634
36	0.5471210214166425
37	0.5425173982672746
38	0.593842898911292
39	0.5681556612321058
40	0.605205032641647
41	0.5564751918584435
42	0.5464108880584955
43	0.5910513401930584
44	0.5634785760112054
45	0.5744244246695431
46	0.5682536106608158
47	0.566612957729924
48	0.6028542463526082
49	0.5730286453104263
50	0.6364264130429459
51	0.6789854398174222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4083740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	64.08099740398278
#Duplication Level	Relative count
1	100.0
2	3.2784412902627045
3	0.9582422171822342
4	0.5719290715143359
5	0.45649916762858256
6	0.3364288655966682
7	0.2575420971119322
8	0.2471012012830701
9	0.20591766773589174
10++	9.697852075708095
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	967815	3.6322172	3.8461134	36
TTTGA	590370	2.1263752	7.796047	8
TGTTT	584395	1.9523175	7.2943983	6
CTTTG	402255	1.7782716	8.9828	1
GTTTG	408375	1.7689915	8.639306	7
TTGAG	328750	1.5353379	6.052826	9
TGTGT	323475	1.4012231	8.073517	4
GTGTT	310835	1.3464695	8.267784	5
TTTGT	396780	1.3255428	6.6411653	2
AACTT	302200	1.1975998	5.3452077	2
TTGTG	242675	1.0512152	7.978188	3
>>END_MODULE