##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane8_Undetermined_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4083740	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.725103214210506	34.0	31.0	34.0	31.0	34.0
2	32.9825461464246	34.0	31.0	34.0	31.0	34.0
3	33.07403238208113	34.0	33.0	34.0	31.0	34.0
4	36.512996664821955	37.0	37.0	37.0	35.0	37.0
5	36.45042656976203	37.0	37.0	37.0	35.0	37.0
6	36.46086430575894	37.0	37.0	37.0	35.0	37.0
7	36.45768339806158	37.0	37.0	37.0	35.0	37.0
8	36.450537742363615	37.0	37.0	37.0	35.0	37.0
9	38.2428893612228	39.0	39.0	39.0	37.0	39.0
10	38.24093869835004	39.0	39.0	39.0	37.0	39.0
11	38.23892779657863	39.0	39.0	39.0	37.0	39.0
12	38.21295038371689	39.0	38.0	39.0	37.0	39.0
13	38.18162982951902	39.0	38.0	39.0	37.0	39.0
14	39.763930612624705	41.0	40.0	41.0	38.0	41.0
15	39.759774128617394	41.0	40.0	41.0	38.0	41.0
16	39.739835787782766	41.0	40.0	41.0	38.0	41.0
17	39.7238903554095	41.0	40.0	41.0	38.0	41.0
18	39.72342803410599	41.0	40.0	41.0	38.0	41.0
19	39.736362501040716	41.0	40.0	41.0	38.0	41.0
20	39.71288794095609	41.0	40.0	41.0	38.0	41.0
21	39.67939070557871	41.0	40.0	41.0	38.0	41.0
22	39.64109027509097	41.0	40.0	41.0	37.0	41.0
23	39.60526061894244	41.0	40.0	41.0	37.0	41.0
24	39.575455097533144	41.0	40.0	41.0	37.0	41.0
25	39.529868698790814	41.0	40.0	41.0	37.0	41.0
26	39.46337034189248	41.0	39.0	41.0	37.0	41.0
27	39.35748407097416	41.0	39.0	41.0	37.0	41.0
28	39.305570628884304	40.0	39.0	41.0	37.0	41.0
29	39.249699785001006	40.0	39.0	41.0	36.0	41.0
30	39.19323047010828	40.0	39.0	41.0	36.0	41.0
31	39.12403948341471	40.0	39.0	41.0	36.0	41.0
32	39.020811070244434	40.0	39.0	41.0	36.0	41.0
33	38.94944609598065	40.0	39.0	41.0	36.0	41.0
34	39.022934613858865	40.0	39.0	41.0	36.0	41.0
35	39.01951764803832	40.0	39.0	41.0	36.0	41.0
36	39.002681120737364	40.0	39.0	41.0	36.0	41.0
37	38.99202471264086	40.0	39.0	41.0	36.0	41.0
38	38.96228946994667	40.0	39.0	41.0	36.0	41.0
39	38.876589841664746	40.0	39.0	41.0	35.0	41.0
40	38.6894234206879	40.0	39.0	41.0	35.0	41.0
41	38.673759348048605	40.0	38.0	41.0	35.0	41.0
42	38.49452707567083	40.0	38.0	41.0	35.0	41.0
43	38.455033376267835	40.0	38.0	41.0	35.0	41.0
44	38.497189096269594	40.0	38.0	41.0	35.0	41.0
45	38.46974317659792	40.0	38.0	41.0	35.0	41.0
46	38.36392938825684	40.0	38.0	41.0	35.0	41.0
47	38.29577593088688	40.0	38.0	41.0	35.0	41.0
48	38.23267911277407	40.0	38.0	41.0	34.0	41.0
49	38.16785960908383	40.0	38.0	41.0	34.0	41.0
50	38.24351134009511	40.0	38.0	41.0	35.0	41.0
51	37.88395563870374	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	7.0
13	13.0
14	24.0
15	49.0
16	88.0
17	157.0
18	395.0
19	747.0
20	1395.0
21	2043.0
22	3470.0
23	5291.0
24	7454.0
25	9661.0
26	11341.0
27	12809.0
28	14225.0
29	16093.0
30	18884.0
31	23256.0
32	30164.0
33	40587.0
34	59703.0
35	96575.0
36	167706.0
37	346070.0
38	919095.0
39	2295386.0
40	1042.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.28043647792334	32.295981448316674	25.554047354993948	22.869534718766044
2	20.9285884650176	31.18690436718628	27.80057311024708	20.083934057549044
3	19.547649045990564	29.318253335635248	29.097808159801936	22.03628945857225
4	20.12627958009027	29.20745577606121	28.79657441288054	21.869690230967986
5	23.32491784491667	29.11142727010045	27.217501603921896	20.34615328106099
6	22.613857671757753	28.237912291214307	27.25727549683826	21.89095454018968
7	23.455680332244462	28.867288319040878	26.51143314706617	21.16559820164849
8	22.201046099898623	28.747275781514002	27.388056046663106	21.663622071924266
9	21.934868527379315	28.405628174173675	27.95119179967383	21.708311498773185
10	22.111765195629495	29.00373187323385	27.207265888621706	21.67723704251495
11	23.014258015572988	28.724149296551342	26.706463539041923	21.55512914883374
12	22.161259571191987	29.390443414723617	26.669064795766822	21.779232218317578
13	22.468545989705515	29.174580164261194	26.742593798821666	21.614280047211626
14	22.423720860521364	29.1067844278587	26.905790719840756	21.56370399177918
15	22.370447438339095	28.61934530107451	27.007285862077072	22.002921398509322
16	22.330493209736403	29.025294169652625	26.828918859943972	21.815293760666997
17	22.565598638784312	28.98351409478887	26.66501911301034	21.785868153416473
18	22.28640902701935	28.63409521663965	26.80415501476588	22.275340741575125
19	22.244731545103264	28.979930162057332	26.771758241220056	22.00358005161935
20	22.49259257445381	28.968445591541087	26.67510664244051	21.863855191564596
21	22.495271972685067	28.63547527724741	26.56515886490123	22.3040938851663
22	22.314332531140188	29.056085521119833	26.614339216763334	22.01524273097665
23	22.57552159858948	28.77950827701048	26.736139680673915	21.908830443726124
24	22.41618948923241	28.60299980214196	26.70699085347339	22.273819855152244
25	22.396514908122086	28.976339967872118	26.51713650432943	22.110008619676368
26	22.58404562800596	28.818170084187074	26.555604295051765	22.042179992755205
27	22.61554285394997	28.624538322396354	26.463969235135217	22.295949588518464
28	22.560476746814206	29.006309754664727	26.25107225669415	22.182141241826912
29	22.849857457907106	28.99082353137077	26.277846642241144	21.881472368480985
30	22.554824521123468	28.793793187052973	26.439715374131666	22.21166691769189
31	22.435490121247366	28.99237164039371	26.37947108537678	22.192667152982146
32	22.736899639578475	29.033062945701477	26.35716903046487	21.872868384255177
33	22.64375361887537	28.71155044510022	26.3587973052279	22.285898630796513
34	22.466489410155184	28.972084923039752	26.367253917865696	22.19417174893937
35	22.81553183387178	28.808038620757433	26.404854619381624	21.971574925989167
36	22.810017198666817	28.62491909361283	26.204514165275576	22.360549542444772
37	22.672625109900373	28.93776782470075	26.202014664497998	22.187592400900876
38	22.87255738024164	28.80768835516065	26.373708038617384	21.94604622598033
39	22.80390408324417	28.543263841279966	26.397431903997827	22.255400171478044
40	22.716654769257357	29.13180496627649	25.931140020561077	22.220400243905072
41	22.70930871092296	29.09949742076484	26.343653140475258	21.847540727836943
42	22.740973951556086	28.914099340003936	26.215401283354332	22.129525425085646
43	22.684417234636207	29.22440010405099	26.01564547598046	22.075537185332347
44	22.804219642799318	29.108195440838948	26.14827822049258	21.939306695869163
45	22.806706620339064	28.807047817991915	26.188539931785016	22.197705629884005
46	22.71992475244897	28.98283857527962	26.071173116298795	22.226063555972615
47	22.812034946823523	28.885532721445443	26.181258094488857	22.121174237242176
48	22.655933954149663	28.828119170028003	26.17578875225344	22.340158123568894
49	22.44098235038446	29.229618662734282	26.098649076900283	22.230749909980975
50	22.584109832139823	28.955052184695024	26.304359721900838	22.156478261264315
51	22.643504282816128	28.68605134165557	26.340358271605968	22.330086103922326
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.149971196689386
2	41.01252252256665
3	41.58393850456281
4	41.99596981105826
5	43.671071125977655
6	44.50481221194743
7	44.62127853389295
8	43.86466817182289
9	43.64318002615249
10	43.78900223814445
11	44.56938716440673
12	43.940491789509565
13	44.08282603691714
14	43.98742485230054
15	44.373368836848414
16	44.1457869704034
17	44.35146679220078
18	44.561749768594474
19	44.24831159672261
20	44.356447766018405
21	44.79936585785136
22	44.32957526211683
23	44.48435204231561
24	44.69000934438465
25	44.50652352779846
26	44.62622562076116
27	44.91149244246843
28	44.74261798864112
29	44.73132982638809
30	44.76649143881536
31	44.62815727422951
32	44.60976802383366
33	44.92965224967188
34	44.66066115909456
35	44.78710675986095
36	45.17056674111159
37	44.860217510801256
38	44.81860360622197
39	45.05930425472221
40	44.93705501316243
41	44.556849438759905
42	44.87049937664173
43	44.75995441996856
44	44.74352633866848
45	45.00441225022307
46	44.94598830842158
47	44.933209184065696
48	44.99609207771856
49	44.67173226036543
50	44.740588093404135
51	44.97359038673846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2754.0
1	1596.5
2	439.0
3	521.5
4	604.0
5	904.0
6	1204.0
7	1735.5
8	2267.0
9	1745.0
10	1223.0
11	1480.0
12	1737.0
13	2232.0
14	2727.0
15	3880.5
16	5034.0
17	7396.5
18	9759.0
19	12236.0
20	14713.0
21	17062.0
22	19411.0
23	23010.5
24	26610.0
25	34187.5
26	53867.0
27	65969.0
28	66807.5
29	67646.0
30	77290.0
31	86934.0
32	102543.0
33	118152.0
34	143275.0
35	168398.0
36	204390.5
37	240383.0
38	263752.5
39	287122.0
40	318800.0
41	350478.0
42	389394.5
43	428311.0
44	440232.0
45	452153.0
46	426936.5
47	401720.0
48	373604.5
49	345489.0
50	310904.5
51	276320.0
52	238901.0
53	201482.0
54	171046.0
55	140610.0
56	119672.5
57	98735.0
58	80476.5
59	62218.0
60	56112.5
61	50007.0
62	40009.5
63	30012.0
64	25632.0
65	21252.0
66	17592.5
67	13933.0
68	12881.5
69	11830.0
70	10274.5
71	8719.0
72	7505.5
73	6292.0
74	5291.5
75	3624.0
76	2957.0
77	2784.0
78	2611.0
79	2618.5
80	2626.0
81	2251.0
82	1876.0
83	1674.0
84	1472.0
85	1318.5
86	1165.0
87	1428.5
88	1692.0
89	1057.0
90	422.0
91	247.5
92	73.0
93	52.0
94	31.0
95	22.0
96	13.0
97	14.5
98	16.0
99	49.5
100	83.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021450924887480592
2	0.0014202667162943772
3	0.004530161077835514
4	0.025785187107896193
5	0.0
6	0.0016406529308917808
7	0.0
8	0.0
9	0.0
10	0.0
11	4.897471435497852E-5
12	2.448735717748926E-5
13	0.0
14	0.010970336015515189
15	0.018708340883601796
16	0.011435595801887485
17	0.02250388124611263
18	0.0
19	0.0
20	0.0
21	0.0027670713610562867
22	0.008595062369298731
23	0.0034282300048484966
24	9.794942870995704E-5
25	0.0014692414306493558
26	0.019810271956588812
27	0.0793635246122427
28	0.0015427035021818236
29	8.32570144034635E-4
30	0.011876368231082292
31	0.032862033332190586
32	0.09292952048857174
33	0.10857694172498739
34	0.26747540244971524
35	0.3278857126065812
36	0.23476029326058956
37	0.12356320431761082
38	0.1593882078682776
39	0.23872724512334279
40	0.24546126834715237
41	0.336333850832815
42	0.28020882818200965
43	0.30998545450983656
44	0.1806187465411608
45	0.24132290498415668
46	0.34216184184105747
47	0.30112103121158545
48	0.243992026916503
49	0.16710172537918672
50	0.2082159980801912
51	0.4311488978240534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4083740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	65.69364965387022
#Duplication Level	Relative count
1	100.0
2	3.392083583551485
3	0.9235236157348834
4	0.5748701294915373
5	0.4605957426960593
6	0.3632293008856265
7	0.29151629284560715
8	0.24195852306185392
9	0.2163050892914405
10++	10.142434860684364
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	818255	3.1746728	3.7018657	13
GCGGC	315130	3.0431416	3.5684245	7
TGTTT	417630	1.929189	10.821826	6
GTTTG	312550	1.7189976	12.150266	7
TTTGA	364700	1.5476575	9.553173	8
TGTGT	276030	1.518141	11.830973	4
TTTGT	322290	1.4887779	10.272404	2
CTTTG	254910	1.4299536	12.128323	1
TTGAG	263315	1.330416	7.1303525	9
GTGTT	238245	1.3103268	11.847186	5
TTGTG	207020	1.1385919	11.671377	3
>>END_MODULE