FastQCFastQC Report
Tue 12 Aug 2014
lane7_Undetermined_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane7_Undetermined_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5614055
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2171085 4.3326764 4.7253428 36
TTTTT 1357575 3.014207 3.437284 14
TGTTT 774560 2.176268 10.477473 6
TTTGA 739465 2.0338035 10.175188 8
CTTTG 520905 1.9198427 13.080224 1
GTTTG 533310 1.8962041 12.373288 7
TGTGT 505665 1.7979113 12.049879 4
TTTGT 553230 1.5544009 9.977666 2
TGAGG 352360 1.551937 5.0639477 10
TTGAG 426155 1.4832247 7.768563 9
GTGTT 415695 1.4780195 11.977712 5
TTGTG 353070 1.2553539 11.854165 3
Produced by FastQC (version 0.10.1)