##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane7_Undetermined_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5614055	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.639834664961423	33.0	31.0	34.0	23.0	34.0
2	30.553364368535757	34.0	31.0	34.0	23.0	34.0
3	30.578387101658247	34.0	31.0	34.0	22.0	34.0
4	33.77823765531332	37.0	35.0	37.0	24.0	37.0
5	33.80304147358727	37.0	35.0	37.0	25.0	37.0
6	33.8081858834657	37.0	35.0	37.0	25.0	37.0
7	33.80960802842152	37.0	35.0	37.0	25.0	37.0
8	33.84949630881778	37.0	35.0	37.0	25.0	37.0
9	35.45547808135118	39.0	37.0	39.0	26.0	39.0
10	35.43054779477579	39.0	37.0	39.0	26.0	39.0
11	35.41918470695424	39.0	37.0	39.0	25.0	39.0
12	35.384901287928244	39.0	37.0	39.0	25.0	39.0
13	35.37437520651294	39.0	37.0	39.0	25.0	39.0
14	36.76206342830628	40.0	38.0	41.0	25.0	41.0
15	36.73062429919194	40.0	38.0	41.0	25.0	41.0
16	36.702937716142785	40.0	38.0	41.0	25.0	41.0
17	36.70641969129266	40.0	38.0	41.0	25.0	41.0
18	36.681433651790016	40.0	38.0	41.0	25.0	41.0
19	36.70431052064862	40.0	38.0	41.0	25.0	41.0
20	36.669970992446636	40.0	38.0	41.0	25.0	41.0
21	36.64455709821154	40.0	38.0	41.0	25.0	41.0
22	36.59428683901387	40.0	38.0	41.0	25.0	41.0
23	36.57730000863903	40.0	38.0	41.0	25.0	41.0
24	36.512341970287075	40.0	38.0	41.0	24.0	41.0
25	36.46042762317077	40.0	38.0	41.0	24.0	41.0
26	36.42141108343257	40.0	38.0	41.0	24.0	41.0
27	36.37671967944739	40.0	38.0	41.0	24.0	41.0
28	36.334054618275026	40.0	38.0	41.0	24.0	41.0
29	36.247355788284935	40.0	38.0	41.0	23.0	41.0
30	36.23665639185936	40.0	38.0	41.0	23.0	41.0
31	36.196790020760396	40.0	38.0	41.0	23.0	41.0
32	36.14699695674517	40.0	37.0	41.0	23.0	41.0
33	36.03831757971734	40.0	37.0	41.0	22.0	41.0
34	35.916284753177514	40.0	37.0	41.0	20.0	41.0
35	35.885365569094	40.0	37.0	41.0	20.0	41.0
36	35.829487598536176	40.0	37.0	41.0	20.0	41.0
37	35.77260055343241	40.0	37.0	41.0	20.0	41.0
38	35.69878510274659	40.0	37.0	41.0	19.0	41.0
39	35.641933682516466	40.0	36.0	41.0	19.0	41.0
40	35.559442328227995	40.0	36.0	41.0	19.0	41.0
41	35.730785145496434	40.0	37.0	41.0	20.0	41.0
42	35.76060031474576	40.0	37.0	41.0	20.0	41.0
43	35.73662370603779	40.0	37.0	41.0	20.0	41.0
44	35.68760815488982	40.0	37.0	41.0	20.0	41.0
45	35.63791020928723	40.0	36.0	41.0	20.0	41.0
46	35.56543870695958	40.0	36.0	41.0	19.0	41.0
47	35.50528521719149	40.0	36.0	41.0	19.0	41.0
48	35.4178343461188	40.0	36.0	41.0	18.0	41.0
49	35.34255916623546	40.0	36.0	41.0	18.0	41.0
50	35.23024106461372	40.0	35.0	41.0	18.0	41.0
51	33.922425234523	38.0	34.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2287.0
3	1735.0
4	2720.0
5	2342.0
6	5124.0
7	19508.0
8	69290.0
9	99825.0
10	99197.0
11	60736.0
12	34700.0
13	25589.0
14	21232.0
15	18770.0
16	17165.0
17	16063.0
18	15415.0
19	14106.0
20	14000.0
21	14408.0
22	15279.0
23	16006.0
24	17437.0
25	18705.0
26	20544.0
27	22671.0
28	25607.0
29	29820.0
30	35081.0
31	42764.0
32	54395.0
33	72056.0
34	100782.0
35	148955.0
36	238956.0
37	449110.0
38	1159683.0
39	2591619.0
40	373.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.17955875569612	29.19331479122129	29.5617755744049	22.06535087867768
2	22.06779116259566	30.691679325851794	28.54449603244461	18.69603347910793
3	21.6504859391705	29.27428719139665	28.497733580208283	20.577493289224567
4	21.732553292476258	27.951440262715305	28.987564687543976	21.328441757264454
5	23.474608912619466	28.449870864050357	28.234451012990665	19.84106921033951
6	22.104051595785414	28.086705081590807	28.793878364481717	21.01536495814206
7	23.183674241802095	28.481222600149824	28.096865946311112	20.238237211736973
8	21.945498040166196	28.389577228839087	28.99188633624326	20.67303839475145
9	21.861712644441162	28.107702124834848	29.23733924909511	20.793245981628875
10	21.954604677413162	28.661599722094966	28.715144663321446	20.668650937170423
11	22.883098428243137	28.3931534237437	27.994841145845896	20.72890700216727
12	21.850119093143658	29.244584940326497	28.014107750735178	20.891188215794667
13	22.3251601095932	28.421111750647217	28.24886627419262	21.00486186556697
14	22.375741122042104	28.661932518457334	27.997856423641615	20.964469935858947
15	22.155776119178757	28.751789376842584	27.882926644313017	21.20950785966564
16	22.298152479684433	28.48700116727863	28.03649422845566	21.17835212458128
17	22.358694086826695	28.54984975230303	27.97205096236071	21.11940519850957
18	22.168659330120633	28.426835050629172	27.944000803234946	21.460504816015245
19	22.08678163651028	28.482534572965495	28.118177916541743	21.312505873982484
20	22.15613919500821	28.54758192334242	28.04004950629476	21.256229375354614
21	22.03562496470812	28.47055888758562	28.00636790615326	21.487448241553
22	22.052995556861607	28.504724934573773	28.072136962417705	21.370142546146916
23	22.23276529268801	28.487049158791493	27.89146682431294	21.388718724207557
24	22.030735859443404	28.504617809422523	27.932004439727343	21.532641891406726
25	22.11518591901978	28.56532781710056	27.878235787489658	21.441250476390003
26	22.10402976627727	28.575065240568343	27.878802603334485	21.442102389819897
27	22.140884192578426	28.68790677823332	27.68158090830338	21.489628120884873
28	22.112509077241818	28.6173912903809	27.721544040394775	21.54855559198251
29	22.21782535203223	28.598707355862686	27.683396787488373	21.500070504616716
30	22.047222181091684	28.58454588642964	27.68904384101284	21.679188091465836
31	22.009684150452106	28.565158459809886	27.82982290310499	21.595334486633018
32	22.240591017320245	28.609900845969538	27.629831375174586	21.519676761535635
33	22.097428348576695	28.561463048105058	27.627612062475738	21.71349654084251
34	22.079936960649814	28.61631347254881	27.658067375632612	21.645682191168763
35	22.19383195118639	28.62970360735969	27.591049120119298	21.58541532133463
36	22.153738898400682	28.603957780997153	27.538175615705722	21.704127704896443
37	22.212014748864544	28.504635771543622	27.57102176649254	21.712327713099295
38	22.242707251133307	28.598940392086796	27.616870441780193	21.541481914999704
39	22.103286482439056	28.502318802505027	27.57549574984636	21.818898965209552
40	22.065928377383496	28.641117053200826	27.582925086889414	21.710029482526263
41	22.172019062367855	28.524387980851735	27.590023447693106	21.713569509087304
42	22.014920899262545	28.44221037183064	27.638989327310426	21.903879401596384
43	22.066641416383376	28.52382858255697	27.669519375893188	21.740010625166466
44	22.16833763370167	28.5265182620559	27.568298730861958	21.736845373380472
45	22.25879016360166	28.358110371021006	27.589586931628908	21.793512533748423
46	22.10943643535906	28.42706510499345	27.6757159766787	21.787782482968787
47	22.208116171649298	28.47058810895137	27.557153220406573	21.764142498992758
48	22.172165648725674	28.46346709616293	27.4588941497725	21.9054731053389
49	22.006587558936676	28.582515286540204	27.60833889692834	21.802558257594775
50	22.093966477465166	28.53465045423943	27.660219630400647	21.711163437894758
51	22.188778798896003	28.050906698760887	27.711828485579638	22.048486016763473
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.244909634373805
2	40.76382464170359
3	42.22797922839507
4	43.06099504974071
5	43.31567812295897
6	43.11941655392747
7	43.42191145353907
8	42.61853643491765
9	42.65495862607004
10	42.623255614583584
11	43.612005430410406
12	42.741307308938325
13	43.330021975160165
14	43.34021105790105
15	43.365283978844396
16	43.476504604265706
17	43.478099285336256
18	43.62916414613588
19	43.39928751049276
20	43.41236857036282
21	43.52307320626112
22	43.423138103008526
23	43.621484016895565
24	43.56337775085013
25	43.55643639540978
26	43.54613215609717
27	43.6305123134633
28	43.661064669224324
29	43.717895856648944
30	43.72641027255752
31	43.605018637085124
32	43.760267778855884
33	43.810924889419205
34	43.72561915181857
35	43.77924727252102
36	43.857866603297126
37	43.92434246196384
38	43.78418916613301
39	43.92218544764861
40	43.77595785990976
41	43.88558857145516
42	43.918800300858926
43	43.806652041549846
44	43.905183007082144
45	44.052302697350086
46	43.89721891832785
47	43.972258670642056
48	44.07763875406457
49	43.809145816531455
50	43.80512991535993
51	44.23726481565948
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2887.0
1	3188.0
2	3489.0
3	3819.5
4	4150.0
5	4907.0
6	5664.0
7	6097.5
8	6531.0
9	6118.0
10	5705.0
11	6123.5
12	6542.0
13	7595.0
14	8648.0
15	10813.5
16	12979.0
17	17927.0
18	22875.0
19	27696.0
20	32517.0
21	37952.5
22	43388.0
23	50226.5
24	57065.0
25	69610.5
26	98380.5
27	114605.0
28	123979.0
29	133353.0
30	149916.5
31	166480.0
32	181576.5
33	196673.0
34	230521.5
35	264370.0
36	299803.0
37	335236.0
38	375015.5
39	414795.0
40	438949.5
41	463104.0
42	495425.5
43	527747.0
44	540413.5
45	553080.0
46	531668.5
47	510257.0
48	451532.5
49	392808.0
50	354171.0
51	315534.0
52	278893.5
53	242253.0
54	211590.0
55	180927.0
56	160873.0
57	140819.0
58	121676.5
59	102534.0
60	95665.5
61	88797.0
62	71332.0
63	53867.0
64	45685.5
65	37504.0
66	30998.0
67	24492.0
68	20372.0
69	16252.0
70	14176.0
71	12100.0
72	10229.5
73	8359.0
74	7117.0
75	4934.0
76	3993.0
77	3618.0
78	3243.0
79	3054.5
80	2866.0
81	2545.5
82	2225.0
83	1915.5
84	1606.0
85	1384.0
86	1162.0
87	1255.0
88	1348.0
89	965.5
90	583.0
91	432.0
92	281.0
93	201.5
94	122.0
95	97.0
96	72.0
97	59.5
98	47.0
99	68.5
100	90.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022194296279605383
2	0.132773904067559
3	0.15172633684564898
4	0.136835139662864
5	0.1553600739572377
6	0.21182549868143438
7	0.2270551321638281
8	0.20099553709395437
9	0.17889030299845654
10	0.2675428010591275
11	0.2972539456774114
12	0.365760577692951
13	0.4131950969486405
14	0.3502993825318776
15	0.4047520018952433
16	0.4298141005031123
17	0.3081017197017129
18	0.29727175811423295
19	0.30888546692185953
20	0.2918211524468499
21	0.3182548086899754
22	0.3488743875861565
23	0.20731895216559154
24	0.27632433241213344
25	0.35444968031129015
26	0.290538656995701
27	0.2950452035115438
28	0.19159057045219544
29	0.20635708057722982
30	0.15311570691772702
31	0.12388549809362395
32	0.1520113358347932
33	0.339344733886647
34	0.49345793726637877
35	0.45040527746878145
36	0.4572630656450641
37	0.4552324478474115
38	0.5059444554782595
39	0.4677011536224708
40	0.48727702168931375
41	0.46084336544618815
42	0.4640852289477036
43	0.48661796152691766
44	0.4590977466376799
45	0.4898063877179686
46	0.4697673962937663
47	0.48114954342271316
48	0.5093822557848116
49	0.4567465049772401
50	0.5480174312506736
51	0.5727945308693984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5614055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	51.009065903777596
#Duplication Level	Relative count
1	100.0
2	3.488707574061465
3	0.9082411051228492
4	0.5245178936192312
5	0.3929556496751336
6	0.2850515285455448
7	0.21638526964489735
8	0.19157309205894912
9	0.16849199663016007
10++	9.229553034587022
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2171085	4.3326764	4.7253428	36
TTTTT	1357575	3.014207	3.437284	14
TGTTT	774560	2.176268	10.477473	6
TTTGA	739465	2.0338035	10.175188	8
CTTTG	520905	1.9198427	13.080224	1
GTTTG	533310	1.8962041	12.373288	7
TGTGT	505665	1.7979113	12.049879	4
TTTGT	553230	1.5544009	9.977666	2
TGAGG	352360	1.551937	5.0639477	10
TTGAG	426155	1.4832247	7.768563	9
GTGTT	415695	1.4780195	11.977712	5
TTGTG	353070	1.2553539	11.854165	3
>>END_MODULE