FastQCFastQC Report
Tue 12 Aug 2014
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane7_Undetermined_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5614055
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTTT 647230 1.9833432 13.079139 6
TGTGT 499785 1.918519 15.582235 4
GTTTG 464220 1.781996 15.480304 7
TTTGA 547085 1.5918565 11.940029 8
CTTTG 404370 1.5860966 15.639278 1
TTTGT 516020 1.5812693 12.641419 2
TGAGG 314000 1.4337254 5.727433 10
GTGTT 364170 1.3979353 15.308985 5
TTGAG 375900 1.3701403 9.212217 9
TTGTG 334615 1.284483 15.153884 3
Produced by FastQC (version 0.10.1)