##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane7_Undetermined_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5614055	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.446157723784324	34.0	31.0	34.0	31.0	34.0
2	32.802655121832615	34.0	31.0	34.0	31.0	34.0
3	32.94705413466737	34.0	31.0	34.0	31.0	34.0
4	36.440196079304535	37.0	37.0	37.0	35.0	37.0
5	36.368054107058086	37.0	37.0	37.0	35.0	37.0
6	36.35833760089632	37.0	37.0	37.0	35.0	37.0
7	36.34345691305126	37.0	37.0	37.0	35.0	37.0
8	36.34804468427901	37.0	37.0	37.0	35.0	37.0
9	38.109632520522155	39.0	38.0	39.0	37.0	39.0
10	38.105607800422334	39.0	38.0	39.0	37.0	39.0
11	38.08737427759436	39.0	38.0	39.0	37.0	39.0
12	38.07887453899187	39.0	38.0	39.0	37.0	39.0
13	38.058935653462605	39.0	38.0	39.0	37.0	39.0
14	39.60666951071908	41.0	40.0	41.0	37.0	41.0
15	39.6023923883895	41.0	40.0	41.0	37.0	41.0
16	39.594199379949075	41.0	40.0	41.0	37.0	41.0
17	39.54327967930489	41.0	40.0	41.0	37.0	41.0
18	39.52962092462578	41.0	40.0	41.0	37.0	41.0
19	39.543825630493465	41.0	40.0	41.0	37.0	41.0
20	39.51745111154059	41.0	40.0	41.0	37.0	41.0
21	39.49171570282087	41.0	39.0	41.0	37.0	41.0
22	39.45138228962844	41.0	39.0	41.0	37.0	41.0
23	39.41601427132438	41.0	39.0	41.0	37.0	41.0
24	39.38765509066085	41.0	39.0	41.0	37.0	41.0
25	39.33882585760204	41.0	39.0	41.0	36.0	41.0
26	39.248755311446004	40.0	39.0	41.0	36.0	41.0
27	39.1566324163194	40.0	39.0	41.0	36.0	41.0
28	39.095532373658614	40.0	39.0	41.0	36.0	41.0
29	39.03149025080802	40.0	39.0	41.0	36.0	41.0
30	38.96721140067206	40.0	39.0	41.0	36.0	41.0
31	38.887784854263096	40.0	39.0	41.0	36.0	41.0
32	32.35659750394323	40.0	35.0	41.0	2.0	41.0
33	38.70616693281416	40.0	38.0	41.0	35.0	41.0
34	38.7931748085831	40.0	39.0	41.0	36.0	41.0
35	38.78608741809619	40.0	39.0	41.0	35.0	41.0
36	38.77500042304538	40.0	39.0	41.0	35.0	41.0
37	38.74814176205969	40.0	39.0	41.0	35.0	41.0
38	38.69914420147291	40.0	38.0	41.0	35.0	41.0
39	38.5697582228888	40.0	38.0	41.0	35.0	41.0
40	38.43517065650408	40.0	38.0	41.0	35.0	41.0
41	38.43855662261948	40.0	38.0	41.0	35.0	41.0
42	38.434116160244244	40.0	38.0	41.0	35.0	41.0
43	38.37023167745952	40.0	38.0	41.0	35.0	41.0
44	38.305365373157194	40.0	38.0	41.0	34.0	41.0
45	38.277067467276325	40.0	38.0	41.0	34.0	41.0
46	38.175329775002204	40.0	38.0	41.0	34.0	41.0
47	38.10959333316114	40.0	38.0	41.0	34.0	41.0
48	38.03676380085339	40.0	38.0	41.0	34.0	41.0
49	37.98735174486178	40.0	38.0	41.0	34.0	41.0
50	37.77427136000627	40.0	37.0	41.0	33.0	41.0
51	37.58135109114535	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	11.0
10	5.0
11	18.0
12	10.0
13	20.0
14	23.0
15	45.0
16	129.0
17	201.0
18	510.0
19	1126.0
20	2088.0
21	3873.0
22	6066.0
23	9587.0
24	13466.0
25	17353.0
26	22036.0
27	25270.0
28	29253.0
29	33632.0
30	38251.0
31	45218.0
32	54824.0
33	70785.0
34	100061.0
35	154790.0
36	269860.0
37	565884.0
38	1570163.0
39	2578642.0
40	855.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.164764480020583	30.928648001666204	27.701603010716134	23.204984507597075
2	20.7943634324922	30.332014916134593	29.534017746530804	19.339603904842402
3	19.571269651402755	28.863396086597543	30.281476757659938	21.283857504339764
4	20.252367065957134	28.78665074754937	29.728648656160782	21.232333530332706
5	23.350448116379336	28.824762137171795	27.901187287976338	19.92360245847253
6	22.229566996183983	28.29753780257782	28.350762179128523	21.122133022109672
7	23.308571077411962	29.002744005892357	27.087052050612257	20.601632866083428
8	21.574655039895404	28.629555642044828	28.716195334744672	21.07959398331509
9	21.362152668614755	28.350452569488542	29.098931164728526	21.188463597168177
10	21.490829712213362	28.852086415255997	28.519510407361526	21.137573465169115
11	22.90520506288348	28.69125837143131	27.348747798819883	21.05478876686532
12	21.575940174274383	29.858675937929846	27.35190079776897	21.213483090026802
13	22.287669073423753	29.0367301353478	27.517970522198304	21.15763026903014
14	22.114874563016315	29.00923290014667	27.68038925168871	21.19550328514831
15	22.034855365445527	28.698515232255694	27.751497967025667	21.515131435273112
16	21.984531178852723	28.96137355264836	27.616046443517924	21.438048824980992
17	22.195000267270103	28.924754556955257	27.488623202608558	21.39162197316608
18	21.947769099326635	28.703073173955335	27.63161527550693	21.7175424512111
19	21.895807163880917	28.987494237673612	27.60498121499409	21.51171738345138
20	21.984394003843253	28.970879209746496	27.537105477096883	21.507621309313365
21	21.964002875665926	28.804379191749774	27.45174388903179	21.779874043552518
22	21.922387957607086	29.014024092196188	27.532398916378447	21.531189033818286
23	22.09150070670843	28.87002353913526	27.53743951564422	21.501036238512093
24	21.951192141865373	28.721895314527558	27.513410538371996	21.813502005235076
25	21.96772996537063	29.03494343845658	27.356347966836044	21.640978629336754
26	22.186988567076025	28.87237112001727	27.311545351204515	21.62909496170219
27	22.151378129064646	28.75876394815508	27.286114026880863	21.803743895899412
28	22.1046683816112	29.01811201600631	27.150495734875584	21.72672386750691
29	22.263787677006487	29.038380828397138	27.191182328173753	21.506649166422616
30	22.116164528618388	28.878571719971347	27.218780691632745	21.786483059777524
31	22.034405817110084	29.0019054931709	27.193050466249634	21.77063822346938
32	22.272709493904898	29.035634044570703	27.11536013833613	21.576296323188267
33	22.21260905328656	28.8209938768216	27.135782176649737	21.830614893242103
34	22.10481441979408	29.015247806975676	27.143269856865228	21.736667916365025
35	22.28097691094917	28.893370747106278	27.191713201713625	21.633939140230925
36	22.3223410091731	28.782953503421844	27.02880644040947	21.865899046995587
37	22.27039750343807	28.97805289071603	27.02648138395251	21.72506822189339
38	22.37242262753277	28.858792905657953	27.149114277711274	21.619670189098006
39	22.356048860034157	28.728758066813604	27.10326359313469	21.81192948001755
40	22.262345680664495	29.185480113818347	26.783568319644907	21.768605885872255
41	22.22300389050589	29.063252101532044	27.13048005760856	21.58326395035351
42	22.25647954993935	28.878457471149122	27.081172799475638	21.783890179435886
43	22.272636312175898	29.084982779731405	26.90862216608183	21.733758742010867
44	22.36145515114875	29.064930386507804	26.936899341761496	21.636715120581947
45	22.340309592884555	28.827865321655004	27.031238433966926	21.800586651493518
46	22.31483025146965	28.979306169236956	26.922025165885866	21.783838413407526
47	22.343081484198517	28.926250001338033	27.006847528577755	21.72382098588569
48	22.224771758554272	28.896801365980952	26.96205986846175	21.916367007003025
49	22.079217238882666	29.177062735036703	26.90293948944565	21.84078053663498
50	22.222394661009723	29.11372647236264	26.950523506320355	21.71335536030728
51	22.267465999872798	28.72597158789223	27.092907835375858	21.913654576859116
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.36974898761766
2	40.1339673373346
3	40.855127155742515
4	41.48470059628984
5	43.27405057485187
6	43.351700018293656
7	43.91020394349539
8	42.6542490232105
9	42.55061626578293
10	42.62840317738248
11	43.959993829748804
12	42.789423264301185
13	43.4452993424539
14	43.31037784816462
15	43.549986800718635
16	43.422580003833716
17	43.586622240436185
18	43.66531155053773
19	43.4075245473323
20	43.49201531315662
21	43.74387691921844
22	43.45357699142537
23	43.59253694522052
24	43.76469414710045
25	43.60870859470739
26	43.816083528778215
27	43.95512202496406
28	43.8313922491181
29	43.7704368434291
30	43.90264758839591
31	43.80504404057947
32	43.849005817093165
33	44.04322394652866
34	43.8414823361591
35	43.9149160511801
36	44.18824005616869
37	43.995465725331464
38	43.99209281663078
39	44.167978340051704
40	44.03095156653675
41	43.8062678408594
42	44.04036972937524
43	44.00639505418676
44	43.9981702717307
45	44.14089624437807
46	44.09866866487718
47	44.066902470084216
48	44.1411387655573
49	43.91999777551764
50	43.935750021317
51	44.181120576731914
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1894.0
1	1208.0
2	522.0
3	716.5
4	911.0
5	1149.5
6	1388.0
7	2189.0
8	2990.0
9	2618.0
10	2246.0
11	2644.5
12	3043.0
13	4205.0
14	5367.0
15	7927.5
16	10488.0
17	15960.5
18	21433.0
19	26663.0
20	31893.0
21	37291.5
22	42690.0
23	50393.0
24	58096.0
25	72176.5
26	104716.0
27	123175.0
28	128061.5
29	132948.0
30	146243.0
31	159538.0
32	180553.5
33	201569.0
34	232214.0
35	262859.0
36	304497.5
37	346136.0
38	372815.5
39	399495.0
40	434095.5
41	468696.0
42	502674.5
43	536653.0
44	545352.0
45	554051.0
46	524283.0
47	494515.0
48	461758.0
49	429001.0
50	388252.5
51	347504.0
52	304501.5
53	261499.0
54	225778.0
55	190057.0
56	163739.0
57	137421.0
58	114651.0
59	91881.0
60	80648.5
61	69416.0
62	56164.0
63	42912.0
64	35801.5
65	28691.0
66	23780.5
67	18870.0
68	16220.0
69	13570.0
70	11718.0
71	9866.0
72	8286.5
73	6707.0
74	5488.0
75	3638.5
76	3008.0
77	2763.5
78	2519.0
79	2467.0
80	2415.0
81	2091.0
82	1767.0
83	1474.0
84	1181.0
85	1082.0
86	983.0
87	1025.5
88	1068.0
89	687.5
90	307.0
91	191.5
92	76.0
93	52.0
94	28.0
95	19.5
96	11.0
97	18.0
98	25.0
99	87.5
100	150.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.023227417615253144
2	0.0
3	0.00480935794180855
4	0.027003654221413934
5	0.0
6	0.0015318695666501308
7	0.0
8	0.0
9	0.0
10	0.0
11	3.740611732517761E-4
12	0.002654053086405459
13	0.0
14	1.781243682151315E-5
15	0.0020128053608309856
16	0.01245089333823769
17	0.031260826621755576
18	3.918736100732893E-4
19	5.343731046453945E-5
20	0.0010509337724692758
21	9.084342778971705E-4
22	0.0051121693677742734
23	0.0
24	0.0
25	0.010901211334766047
26	0.07488348439764128
27	0.08391438986614844
28	0.004773733068165523
29	0.007463411028214009
30	0.013501827110706967
31	0.06116790804507616
32	17.57171955030722
33	0.1275904849524987
34	0.17876561594070597
35	0.26168250934484966
36	0.19474337176960324
37	0.12440205876144783
38	0.16075724231415617
39	0.08564219623783521
40	0.17253126305317637
41	0.2404322722167845
42	0.15614382117738426
43	0.21777485257981977
44	0.11871989141538514
45	0.13644326605279072
46	0.20856582274309746
47	0.1571235052025675
48	0.09248217197729626
49	0.06699257488571095
50	0.14607979437322932
51	0.2978773809661644
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5614055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	52.923644504533605
#Duplication Level	Relative count
1	100.0
2	3.7474783296222873
3	1.0144818003709108
4	0.5895040375794289
5	0.4110250045055782
6	0.34126121307606
7	0.29068246428965927
8	0.235452796074624
9	0.2011522652884442
10++	9.441947805056712
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTTT	647230	1.9833432	13.079139	6
TGTGT	499785	1.918519	15.582235	4
GTTTG	464220	1.781996	15.480304	7
TTTGA	547085	1.5918565	11.940029	8
CTTTG	404370	1.5860966	15.639278	1
TTTGT	516020	1.5812693	12.641419	2
TGAGG	314000	1.4337254	5.727433	10
GTGTT	364170	1.3979353	15.308985	5
TTGAG	375900	1.3701403	9.212217	9
TTGTG	334615	1.284483	15.153884	3
>>END_MODULE