FastQCFastQC Report
Tue 12 Aug 2014
lane6_Undetermined_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane6_Undetermined_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4711860
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1758050 4.6657405 5.010877 36
TTTTT 1422655 3.769414 4.5901613 18
TGTTT 686290 2.2691898 13.033995 6
TTTGA 665555 2.2013552 12.990009 8
GTTTG 497360 2.052215 15.516106 7
CTTTG 475405 2.0349653 16.412916 1
TGTGT 486600 2.0078173 15.220345 4
GAGGG 294125 1.8906226 5.026746 11
GTGTT 402690 1.6615864 15.172694 5
TTTGT 492240 1.6275715 12.591884 2
TGAGG 311055 1.6022164 5.8985915 10
TTGAG 383455 1.5827396 9.397142 9
TTGTG 345510 1.4256492 15.059173 3
ACTTT 368380 1.2639892 5.8527083 3
AACTT 346700 1.1899924 6.0075254 2
TAACT 224190 0.7694964 5.6225495 1
Produced by FastQC (version 0.10.1)