##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane6_Undetermined_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4711860	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.853916500065793	33.0	31.0	34.0	26.0	34.0
2	30.813587840046182	34.0	31.0	34.0	26.0	34.0
3	30.81732373202939	34.0	31.0	34.0	26.0	34.0
4	34.035569605208984	37.0	35.0	37.0	28.0	37.0
5	34.07846264532478	37.0	35.0	37.0	28.0	37.0
6	34.05842703306126	37.0	35.0	37.0	28.0	37.0
7	34.06287071347621	37.0	35.0	37.0	28.0	37.0
8	34.06508257885421	37.0	35.0	37.0	28.0	37.0
9	35.719114320034976	39.0	37.0	39.0	28.0	39.0
10	35.660143552652244	39.0	37.0	39.0	28.0	39.0
11	35.666446795957434	39.0	37.0	39.0	28.0	39.0
12	35.63114799675712	39.0	37.0	39.0	27.0	39.0
13	35.648655944786135	39.0	37.0	39.0	27.0	39.0
14	37.041027110313124	41.0	38.0	41.0	27.0	41.0
15	36.99692138560993	40.0	38.0	41.0	27.0	41.0
16	36.97724189598163	40.0	38.0	41.0	27.0	41.0
17	36.98133497175213	40.0	38.0	41.0	27.0	41.0
18	36.93807583417164	40.0	38.0	41.0	27.0	41.0
19	36.929957171902394	40.0	38.0	41.0	27.0	41.0
20	36.91834031571396	40.0	38.0	41.0	27.0	41.0
21	36.897556591239976	40.0	38.0	41.0	27.0	41.0
22	36.84170794548225	40.0	38.0	41.0	27.0	41.0
23	36.808696565687434	40.0	38.0	41.0	27.0	41.0
24	36.76244688933882	40.0	38.0	41.0	27.0	41.0
25	36.688253895489254	40.0	38.0	41.0	27.0	41.0
26	36.64179793117792	40.0	38.0	41.0	26.0	41.0
27	36.618746312496555	40.0	38.0	41.0	26.0	41.0
28	36.599039020683975	40.0	38.0	41.0	26.0	41.0
29	36.50878931037849	40.0	38.0	41.0	25.0	41.0
30	36.46491958589601	40.0	38.0	41.0	25.0	41.0
31	36.44384171006779	40.0	38.0	41.0	25.0	41.0
32	36.388209539332664	40.0	38.0	41.0	25.0	41.0
33	36.279778898354365	40.0	38.0	41.0	25.0	41.0
34	36.19355838246467	40.0	37.0	41.0	24.0	41.0
35	36.163967732487805	40.0	37.0	41.0	24.0	41.0
36	36.10495197225724	40.0	37.0	41.0	24.0	41.0
37	36.03129358682134	40.0	37.0	41.0	24.0	41.0
38	35.943823882712984	40.0	37.0	41.0	24.0	41.0
39	35.880399035625	40.0	37.0	41.0	23.0	41.0
40	35.80290055307246	40.0	36.0	41.0	23.0	41.0
41	35.991862661454284	40.0	37.0	41.0	24.0	41.0
42	35.96417253483762	40.0	37.0	41.0	23.0	41.0
43	35.94909674735667	40.0	37.0	41.0	23.0	41.0
44	35.90205332925851	40.0	37.0	41.0	23.0	41.0
45	35.84282448969197	40.0	36.0	41.0	23.0	41.0
46	35.78264146218266	40.0	36.0	41.0	23.0	41.0
47	35.718186660894	40.0	36.0	41.0	23.0	41.0
48	35.62307390287487	40.0	36.0	41.0	22.0	41.0
49	35.53309287627391	40.0	36.0	41.0	22.0	41.0
50	35.437713981315234	40.0	35.0	41.0	22.0	41.0
51	34.089591371560275	38.0	34.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5011.0
3	2497.0
4	2637.0
5	2504.0
6	5475.0
7	15897.0
8	49872.0
9	73224.0
10	74020.0
11	46894.0
12	27276.0
13	19669.0
14	15667.0
15	13749.0
16	12617.0
17	11693.0
18	11330.0
19	11036.0
20	10943.0
21	11531.0
22	11858.0
23	12736.0
24	13639.0
25	14317.0
26	15694.0
27	17810.0
28	20548.0
29	24087.0
30	28316.0
31	34776.0
32	45106.0
33	60680.0
34	85937.0
35	130547.0
36	211685.0
37	390567.0
38	986836.0
39	2182914.0
40	265.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.319080038143042	28.24437291385336	29.943645761706268	22.492901286297332
2	22.148920585680738	30.09923999281347	28.763757788505593	18.988081633000196
3	22.045842465507043	28.70346582355161	28.458794163553907	20.791897547387443
4	21.879369973101966	27.075919997975255	29.23520645467691	21.809503574245863
5	24.17406972052962	27.50614047083359	28.257884249705604	20.061905558931187
6	22.09300543022804	27.23081364847279	29.484643594443043	21.19153732685612
7	23.628545640819247	27.5844471228354	28.406247481902742	20.380759754442614
8	22.32991598410182	27.608299165164546	29.331993224215232	20.729791626518406
9	22.25280651132346	27.342546096287062	29.533755324125682	20.870892068263792
10	22.318487324234752	28.18612499880151	28.70178473837225	20.79360293859149
11	23.525547378287424	27.60319797492597	27.985246352500543	20.886008294286064
12	22.07335602064497	28.86180146708624	27.985953629549833	21.078888882718957
13	22.768302775838993	27.68731406032853	28.346167715531152	21.198215448301333
14	22.9493561550925	27.97890076112568	27.891545187266516	21.180197896515306
15	22.57457408125889	28.301712344196705	27.711756140901926	21.411957433642474
16	22.793602245761843	27.858768831506314	27.90097516942372	21.446653753308123
17	22.727983662115967	27.925922179966378	27.933731794369866	21.412362363547793
18	22.606482551470584	27.81511526459559	27.91724197335239	21.661160210581436
19	22.473941105852898	27.85387971845675	28.11642251645617	21.55575665923418
20	22.39259514931307	28.016267857405865	28.0569951338589	21.534141859422164
21	22.355474164904827	27.85655473887534	28.01839013360968	21.769580962610156
22	22.34877404097187	27.913634772632772	28.088986859591692	21.64860432680367
23	22.540649808785755	27.991523751438958	27.87766465031852	21.590161789456772
24	22.30435189849489	27.989520571926374	27.929224815480264	21.776902714098476
25	22.39210167679401	28.03911572507763	27.839060580166326	21.729722017962036
26	22.4047879777895	28.07569435563229	27.82187960837204	21.697638058206163
27	22.398631706760163	28.09911376765049	27.67462703766221	21.82762748792714
28	22.368412923194526	28.1195826062206	27.660252593158667	21.85175187742621
29	22.454881434329227	28.08560765665699	27.641909696145557	21.81760121286822
30	22.329711921214844	28.059136592455097	27.645455721168528	21.965695765161534
31	22.287013525749522	28.047233222286867	27.760772515445282	21.904980736518333
32	22.502536766601704	28.10598890002135	27.591944737908648	21.799529595468297
33	22.397252978192334	28.01423034484849	27.583247111782622	22.005269565176555
34	22.313819406652026	28.02471991097686	27.651595632120323	22.00986505025079
35	22.502012337785942	28.096802196798524	27.49071080977497	21.910474655640563
36	22.472352622743937	28.016492796142007	27.506450770079354	22.0047038110347
37	22.484736828495784	27.963258545271074	27.519441056665155	22.032563569567987
38	22.589229692062453	28.070874780104184	27.534356783612253	21.805538744221117
39	22.408132920294506	27.96821066006124	27.54293771156768	22.080718708076574
40	22.37383375402112	28.102863777338023	27.499019107885303	22.024283360755547
41	22.43454858899452	27.99421894148076	27.545169054137997	22.026063415386723
42	22.38062972538165	27.96472925885588	27.534956312222043	22.11968470354043
43	22.397230552842018	27.989200951650105	27.58271895754555	22.030849537962332
44	22.473899149886368	27.97594704715166	27.51060215368947	22.0395516492725
45	22.492858689127363	27.86326123317996	27.554849776566243	22.089030301126435
46	22.37538236595548	27.898168277359876	27.585584776758136	22.140864579926507
47	22.480914822856548	28.004712822280165	27.469827589892958	22.044544764970333
48	22.453500120279045	27.867660973458424	27.392895517600834	22.285943388661693
49	22.334684331328628	28.055447316205314	27.48439217053508	22.125476181930978
50	22.38014292199937	27.946154370899635	27.617087769128172	22.056614937972828
51	22.476177475746354	27.54522893736255	27.618979967833678	22.35961361905742
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.811981324440374
2	41.137002218680934
3	42.83774001289448
4	43.688873547347825
5	44.23597527946081
6	43.28454275708417
7	44.00930539526186
8	43.05970761062022
9	43.123698579587256
10	43.11209026282624
11	44.411555672573485
12	43.15224490336393
13	43.96651822414032
14	44.129554051607805
15	43.98653151490136
16	44.240255999069966
17	44.14034602566376
18	44.26764276205202
19	44.029697765087086
20	43.92673700873523
21	44.12505512751498
22	43.99737836777554
23	44.13081159824252
24	44.08125461259336
25	44.121823694756046
26	44.10242603599566
27	44.2262591946873
28	44.220164800620736
29	44.27248264719745
30	44.29540768637638
31	44.191994262267855
32	44.30206636207
33	44.40252254336889
34	44.323684456902825
35	44.412486993426505
36	44.47705643377864
37	44.51730039806377
38	44.39476843628357
39	44.48885162837109
40	44.39811711477667
41	44.46061200438125
42	44.500314428922074
43	44.42808009080435
44	44.51345079915887
45	44.5818889902538
46	44.516246945881996
47	44.52545958782687
48	44.73944350894074
49	44.46016051325961
50	44.4367578599722
51	44.835791094803774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5089.0
1	4798.5
2	4508.0
3	4411.5
4	4315.0
5	6347.0
6	8379.0
7	9457.5
8	10536.0
9	8561.5
10	6587.0
11	6864.0
12	7141.0
13	7824.5
14	8508.0
15	10070.5
16	11633.0
17	14628.0
18	17623.0
19	21244.5
20	24866.0
21	29020.0
22	33174.0
23	38569.5
24	43965.0
25	53446.5
26	75294.0
27	87660.0
28	94589.5
29	101519.0
30	116195.0
31	130871.0
32	143603.0
33	156335.0
34	183988.5
35	211642.0
36	239729.0
37	267816.0
38	300853.0
39	333890.0
40	354601.5
41	375313.0
42	404079.5
43	432846.0
44	445283.0
45	457720.0
46	443251.5
47	428783.0
48	381139.0
49	333495.0
50	302900.0
51	272305.0
52	241931.0
53	211557.0
54	185870.5
55	160184.0
56	144191.5
57	128199.0
58	111170.5
59	94142.0
60	88730.5
61	83319.0
62	68282.5
63	53246.0
64	45631.5
65	38017.0
66	31805.0
67	25593.0
68	22327.5
69	19062.0
70	16926.0
71	14790.0
72	12713.0
73	10636.0
74	9066.5
75	6366.0
76	5235.0
77	4843.0
78	4451.0
79	4321.5
80	4192.0
81	3705.0
82	3218.0
83	2935.5
84	2653.0
85	2407.0
86	2161.0
87	2342.0
88	2523.0
89	1747.0
90	971.0
91	662.0
92	353.0
93	278.0
94	203.0
95	152.5
96	102.0
97	77.0
98	52.0
99	54.5
100	57.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09161986986030994
2	0.18283650193341908
3	0.2913286897318681
4	0.21284588251773184
5	0.2092379654743562
6	0.3223355532634671
7	0.441821276523496
8	0.3337323265122478
9	0.32999707122028243
10	0.3916712296205745
11	0.5309792735777379
12	0.6293480706133034
13	0.4775821013357782
14	0.46336266357659184
15	0.6076368992287546
16	0.6890697092018864
17	0.5375584164215406
18	0.45858747925447696
19	0.6115207158107414
20	0.5738498172696133
21	0.5305548127491054
22	0.6214318761593086
23	0.4078644102329017
24	0.5297907832575671
25	0.6292844014890087
26	0.5969829324300807
27	0.5460688560356207
28	0.38343668954510535
29	0.44209717606210713
30	0.39022806280322425
31	0.33804060392286694
32	0.4006485761461504
33	0.5777548568930316
34	0.7115236870365418
35	0.5468753316100224
36	0.57616312878566
37	0.6888787018290018
38	0.7594028685062799
39	0.7149618197484645
40	0.7427003348995938
41	0.5416332403764119
42	0.6780125046160115
43	0.7145798050026954
44	0.5171842966471839
45	0.6875628732602412
46	0.7162776483172251
47	0.7302424095792319
48	0.747793864843183
49	0.6552826272427449
50	0.7556039440900196
51	0.8070485965202702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4711860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	50.318733825555185
#Duplication Level	Relative count
1	100.0
2	3.2263490977011826
3	0.857343395613511
4	0.43157204758893924
5	0.3422412742976803
6	0.25117029113711115
7	0.24478952161630693
8	0.22854756283607802
9	0.1641597976715992
10++	10.267818298886846
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1758050	4.6657405	5.010877	36
TTTTT	1422655	3.769414	4.5901613	18
TGTTT	686290	2.2691898	13.033995	6
TTTGA	665555	2.2013552	12.990009	8
GTTTG	497360	2.052215	15.516106	7
CTTTG	475405	2.0349653	16.412916	1
TGTGT	486600	2.0078173	15.220345	4
GAGGG	294125	1.8906226	5.026746	11
GTGTT	402690	1.6615864	15.172694	5
TTTGT	492240	1.6275715	12.591884	2
TGAGG	311055	1.6022164	5.8985915	10
TTGAG	383455	1.5827396	9.397142	9
TTGTG	345510	1.4256492	15.059173	3
ACTTT	368380	1.2639892	5.8527083	3
AACTT	346700	1.1899924	6.0075254	2
TAACT	224190	0.7694964	5.6225495	1
>>END_MODULE