FastQCFastQC Report
Tue 12 Aug 2014
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane6_Undetermined_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4711860
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1209260 3.667313 4.415405 16
TGTTT 558355 2.0544982 16.596523 6
TGTGT 450115 2.0094903 19.379711 4
GTTTG 417475 1.8637722 19.31885 7
TTTGA 485795 1.7090113 15.466689 8
CTTTG 370945 1.69658 19.693462 1
TTTGT 451350 1.6607674 16.179274 2
GAGGG 256525 1.611843 5.0454707 11
GTGTT 340245 1.5189872 19.146519 5
TGAGG 278000 1.439696 6.4714313 10
TTGAG 330935 1.4125429 10.9106865 9
TTGTG 308970 1.3793635 19.004005 3