##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane6_Undetermined_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4711860	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.796269413777146	34.0	31.0	34.0	31.0	34.0
2	33.03170297929056	34.0	31.0	34.0	31.0	34.0
3	33.11041393420008	34.0	33.0	34.0	31.0	34.0
4	36.49247643181249	37.0	37.0	37.0	35.0	37.0
5	36.43544226696039	37.0	37.0	37.0	35.0	37.0
6	36.425959175357505	37.0	37.0	37.0	35.0	37.0
7	36.41140632361743	37.0	37.0	37.0	35.0	37.0
8	36.40790027717292	37.0	37.0	37.0	35.0	37.0
9	38.1755574231832	39.0	38.0	39.0	37.0	39.0
10	38.15786037785503	39.0	38.0	39.0	37.0	39.0
11	38.12577899173575	39.0	38.0	39.0	37.0	39.0
12	38.10701060727611	39.0	38.0	39.0	37.0	39.0
13	38.10483588222061	39.0	38.0	39.0	37.0	39.0
14	39.674101098080165	41.0	40.0	41.0	38.0	41.0
15	39.67647829095092	41.0	40.0	41.0	38.0	41.0
16	39.657418301902005	41.0	40.0	41.0	38.0	41.0
17	39.57627539867483	41.0	40.0	41.0	37.0	41.0
18	39.55514722423841	41.0	40.0	41.0	37.0	41.0
19	39.61412372184233	41.0	40.0	41.0	37.0	41.0
20	39.593908987109124	41.0	40.0	41.0	37.0	41.0
21	39.55326261815928	41.0	40.0	41.0	37.0	41.0
22	39.4967030005136	41.0	39.0	41.0	37.0	41.0
23	39.45523805885574	41.0	39.0	41.0	37.0	41.0
24	39.401873782327996	41.0	39.0	41.0	37.0	41.0
25	39.350490888948315	41.0	39.0	41.0	37.0	41.0
26	39.26006163171232	41.0	39.0	41.0	36.0	41.0
27	39.193272720327	40.0	39.0	41.0	36.0	41.0
28	39.12996459996689	40.0	39.0	41.0	36.0	41.0
29	39.04299066610638	40.0	39.0	41.0	36.0	41.0
30	38.99396289363436	40.0	39.0	41.0	36.0	41.0
31	38.90570687584096	40.0	39.0	41.0	36.0	41.0
32	38.807735374141	40.0	38.0	41.0	35.0	41.0
33	38.697901041202414	40.0	38.0	41.0	35.0	41.0
34	38.762342684205386	40.0	39.0	41.0	35.0	41.0
35	38.71401633325269	40.0	39.0	41.0	35.0	41.0
36	38.73749241276269	40.0	39.0	41.0	35.0	41.0
37	38.68546221661934	40.0	39.0	41.0	35.0	41.0
38	38.6547482310595	40.0	38.0	41.0	35.0	41.0
39	38.560512833573156	40.0	38.0	41.0	35.0	41.0
40	38.54988199988964	40.0	38.0	41.0	35.0	41.0
41	38.42063940779225	40.0	38.0	41.0	35.0	41.0
42	38.36008837274452	40.0	38.0	41.0	35.0	41.0
43	38.29529272941047	40.0	38.0	41.0	35.0	41.0
44	38.17674994588124	40.0	38.0	41.0	34.0	41.0
45	38.172068779632674	40.0	38.0	41.0	34.0	41.0
46	38.07969124719325	40.0	38.0	41.0	34.0	41.0
47	38.02480549082528	40.0	38.0	41.0	34.0	41.0
48	37.94978373720781	40.0	38.0	41.0	34.0	41.0
49	37.91345243704185	40.0	37.0	41.0	34.0	41.0
50	37.91653359819689	40.0	37.0	41.0	34.0	41.0
51	37.5869870921462	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	9.0
12	10.0
13	15.0
14	32.0
15	58.0
16	138.0
17	273.0
18	675.0
19	1507.0
20	2265.0
21	3345.0
22	5072.0
23	7769.0
24	11018.0
25	13650.0
26	16320.0
27	19237.0
28	22457.0
29	24763.0
30	28426.0
31	34392.0
32	42192.0
33	55508.0
34	80215.0
35	126497.0
36	213653.0
37	407465.0
38	1032729.0
39	2561086.0
40	1076.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.205260336784676	29.824793423517608	27.479995117524375	23.489951122173338
2	21.636047642625552	29.45058937809951	29.154313896232697	19.75904908304224
3	20.304652020606976	28.11828680407199	29.931605784793163	21.64545539052787
4	20.995150216760646	28.039053852845612	29.398907939031055	21.56688799136269
5	24.005196360849528	28.114892973091365	27.581652626970097	20.298258039089013
6	22.415584658138503	27.62072872101774	28.482444571691012	21.481242049152737
7	23.989040421404713	28.349505290904233	26.78082540652736	20.8806288811637
8	21.997576328668508	28.046164359722063	28.642892615654965	21.313366695954464
9	21.675537897985084	27.854583964718817	29.039721044343423	21.430157092952676
10	21.86752278421856	28.30780247267825	28.38291154956456	21.441763193538623
11	23.602889694104924	28.10386480350628	26.930613681159493	21.362631821229307
12	21.950036195966298	29.70785790976837	26.834358726538674	21.507747167726656
13	22.8204792980234	28.537643546183943	27.142421754754736	21.499455401037924
14	22.595641636211603	28.522409409447647	27.331902900340843	21.550046053999907
15	22.563082539741156	28.206060397686407	27.37495604124366	21.855901021328776
16	22.502138009693443	28.449032141907793	27.26629027504953	21.782539573349233
17	22.638926542680263	28.49242015890077	27.133098798941514	21.735554499477455
18	22.43935515910914	28.200795439592856	27.306711150161505	22.053138251136495
19	22.28527587831557	28.536480285916817	27.35191622841086	21.826327607356756
20	22.353932417346865	28.523449338477796	27.291812575076506	21.830805669098826
21	22.35588269251013	28.315721023732475	27.221099286565632	22.107296997191764
22	22.395967196115084	28.540402851673864	27.171168983812628	21.892460968398424
23	22.530380783809367	28.42525032577369	27.21122868676064	21.833140203656306
24	22.35467522379697	28.368011783032603	27.148811721910242	22.12850127126018
25	22.366613109229995	28.673489578379552	27.00553775969129	21.95435955269916
26	22.521357151750834	28.470571687065195	27.005838450139873	22.0022327110441
27	22.493745685260755	28.27854884183958	27.007745985527322	22.21995948737235
28	22.453334397783678	28.58405880861678	26.853237068520734	22.109369725078803
29	22.67421230058663	28.595397234093383	26.793703713534896	21.936686751785096
30	22.485532052378833	28.440964277935738	26.94141792042809	22.13208574925734
31	22.41875842941638	28.572888256608163	26.819952020967513	22.18840129300795
32	22.634389488178964	28.618520373925254	26.862056588897403	21.88503354899838
33	22.59774091727319	28.378572707976396	26.809301985217125	22.214384389533286
34	22.517142493505453	28.55569730117198	26.7984347538117	22.128725451510867
35	22.72518394107065	28.483455960713766	26.807439489142578	21.983920609073003
36	22.740199905945133	28.310302794144267	26.722281411231407	22.22721588867919
37	22.677204033496974	28.552711168397092	26.685777917587362	22.08430688051857
38	22.73427868470571	28.487249882726363	26.8309100327728	21.94756139979513
39	22.71027121295787	28.253006501941186	26.801227371898744	22.2354949132022
40	22.578864505855123	28.677256257222854	26.559026399865836	22.184852837056184
41	22.573246255980205	28.654336703411442	26.803264057175387	21.969152983432966
42	22.64738147044826	28.430446670839828	26.768216383635675	22.15395547507624
43	22.610684556490206	28.696326432095088	26.547755446242732	22.14523356517197
44	22.790206668664286	28.592691898641952	26.59775797217551	22.019343460518247
45	22.721059414451375	28.399783976806823	26.66858158508505	22.210575023656748
46	22.695718670041916	28.548203665142356	26.563010370733302	22.19306729408242
47	22.676846655458206	28.506480196720602	26.688351396491917	22.128321751329274
48	22.603781056268822	28.435034348589	26.645160563145453	22.316024031996726
49	22.551323857811106	28.698571026918852	26.52523318601623	22.224871929253815
50	22.589476160000817	28.563259873077172	26.693270124732738	22.15399384218927
51	22.687800094915975	28.304537244059375	26.70176807155546	22.305894589469194
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.69521145895801
2	41.39509672566779
3	41.950107411134844
4	42.56203820812333
5	44.30345439993854
6	43.89682670729124
7	44.86966930256841
8	43.31094302462297
9	43.10569499093776
10	43.30928597775719
11	44.96552151533423
12	43.457783363692954
13	44.31993469906132
14	44.14568769021151
15	44.418983561069936
16	44.28467758304267
17	44.37448104215772
18	44.492493410245636
19	44.111603485672326
20	44.18473808644569
21	44.463179689701896
22	44.28842816451351
23	44.36352098746567
24	44.48317649505715
25	44.320972661929154
26	44.523589862794935
27	44.7137051726331
28	44.562704122862485
29	44.61089905237173
30	44.61761780163617
31	44.60715972242432
32	44.51942303717734
33	44.81212530680648
34	44.64586794501632
35	44.70910455014366
36	44.96741579462432
37	44.761510914015545
38	44.68184008450084
39	44.945766126160066
40	44.763717342911306
41	44.54239923941317
42	44.8013369455245
43	44.755918121662184
44	44.80955012918253
45	44.93163443810813
46	44.88878596412434
47	44.80516840678748
48	44.91980508826555
49	44.77619578706492
50	44.74347000219009
51	44.99369468438517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3281.0
1	1962.5
2	644.0
3	854.5
4	1065.0
5	1931.5
6	2798.0
7	4656.0
8	6514.0
9	4439.5
10	2365.0
11	2667.5
12	2970.0
13	3745.5
14	4521.0
15	6339.5
16	8158.0
17	11701.0
18	15244.0
19	19063.5
20	22883.0
21	27200.0
22	31517.0
23	37145.0
24	42773.0
25	52695.0
26	76673.0
27	90729.0
28	94895.5
29	99062.0
30	110088.5
31	121115.0
32	137662.0
33	154209.0
34	177499.5
35	200790.0
36	234326.0
37	267862.0
38	290979.0
39	314096.0
40	343337.5
41	372579.0
42	404899.0
43	437219.0
44	448354.0
45	459489.0
46	439219.0
47	418949.0
48	393959.5
49	368970.0
50	337928.0
51	306886.0
52	271538.0
53	236190.0
54	206424.0
55	176658.0
56	154690.0
57	132722.0
58	112205.5
59	91689.0
60	82317.0
61	72945.0
62	60445.0
63	47945.0
64	40998.5
65	34052.0
66	28471.0
67	22890.0
68	20941.5
69	18993.0
70	17012.0
71	15031.0
72	12805.5
73	10580.0
74	8977.0
75	6181.5
76	4989.0
77	4721.0
78	4453.0
79	4487.5
80	4522.0
81	3872.0
82	3222.0
83	2906.0
84	2590.0
85	2304.0
86	2018.0
87	2364.5
88	2711.0
89	1699.5
90	688.0
91	406.5
92	125.0
93	85.0
94	45.0
95	33.0
96	21.0
97	17.5
98	14.0
99	51.0
100	88.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024088152024890383
2	6.15468201517023E-4
3	0.001230936403034046
4	0.02406692898345876
5	0.0021435271845937696
6	0.002079858060298905
7	0.0
8	0.0
9	0.0
10	9.762599058545881E-4
11	0.00171906635596134
12	1.9100737288459336E-4
13	0.0028863336347005218
14	0.0
15	0.0012097133616024245
16	0.039729533559995416
17	0.18941564477722175
18	0.0
19	0.0
20	0.0
21	8.489216572648593E-5
22	0.0021435271845937696
23	0.0
24	0.0
25	0.03506046444503869
26	0.11617492879669601
27	0.057535665321125834
28	0.044016587929182956
29	0.05804501831548475
30	0.023387791657646873
31	0.09310548276052344
32	0.18863039224425174
33	0.23375057832787902
34	0.282308897123429
35	0.42743205443285665
36	0.21963725577585072
37	0.2845160934323176
38	0.2401387137987971
39	0.1868264337225639
40	0.15231776835474736
41	0.4849252736711193
42	0.4238453604309127
43	0.28907904734011625
44	0.20371997470213463
45	0.1052238394179793
46	0.19941169729151545
47	0.2891214934229795
48	0.20435666594508325
49	0.058236025688369344
50	0.38167517710628074
51	0.5882390393602527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4711860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	52.44527169219676
#Duplication Level	Relative count
1	100.0
2	3.7547676557532164
3	0.9902619345663123
4	0.5400894468050095
5	0.3443878300628243
6	0.298547811726817
7	0.24154163507819248
8	0.20157854216987842
9	0.1892369987717226
10++	10.978096698930987
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1209260	3.667313	4.415405	16
TGTTT	558355	2.0544982	16.596523	6
TGTGT	450115	2.0094903	19.379711	4
GTTTG	417475	1.8637722	19.31885	7
TTTGA	485795	1.7090113	15.466689	8
CTTTG	370945	1.69658	19.693462	1
TTTGT	451350	1.6607674	16.179274	2
GAGGG	256525	1.611843	5.0454707	11
GTGTT	340245	1.5189872	19.146519	5
TGAGG	278000	1.439696	6.4714313	10
TTGAG	330935	1.4125429	10.9106865	9
TTGTG	308970	1.3793635	19.004005	3
>>END_MODULE