FastQCFastQC Report
Tue 12 Aug 2014
lane5_Undetermined_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane5_Undetermined_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5257550
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1919495 4.7919655 5.183801 36
TTTTT 1576240 3.4364247 4.1588707 16
TTTGA 809845 2.3118582 14.159072 8
TGTTT 823010 2.2866302 13.854864 6
GTTTG 596840 2.1132705 16.808659 7
CTTTG 559390 2.0539794 17.549608 1
TGTGT 565400 2.0019488 16.42838 4
GAGGG 314190 1.8563961 5.5954585 11
GTGTT 485845 1.7202634 16.505001 5
TTGAG 460090 1.6738187 10.574535 9
TGAGG 356915 1.6547667 6.5707917 10
TTTGT 574555 1.5963291 13.246814 2
TTGTG 406095 1.4378873 16.25777 3
ACTTT 415490 1.2299972 6.298622 3
AACTT 386690 1.176183 6.595714 2
TAACT 248265 0.75513995 6.2110186 1