##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane5_Undetermined_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5257550	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.366647582999686	34.0	31.0	34.0	28.0	34.0
2	31.3494038097593	34.0	31.0	34.0	28.0	34.0
3	31.35627830453348	34.0	31.0	34.0	28.0	34.0
4	34.57883843234967	37.0	37.0	37.0	32.0	37.0
5	34.61628363020799	37.0	37.0	37.0	32.0	37.0
6	34.65081739593537	37.0	37.0	37.0	32.0	37.0
7	34.652834495154586	37.0	37.0	37.0	32.0	37.0
8	34.65849074188548	37.0	37.0	37.0	32.0	37.0
9	36.33883614991774	39.0	38.0	39.0	32.0	39.0
10	36.3155055111221	39.0	38.0	39.0	32.0	39.0
11	36.33590836035796	39.0	38.0	39.0	32.0	39.0
12	36.28006143545948	39.0	38.0	39.0	32.0	39.0
13	36.27320710216736	39.0	38.0	39.0	32.0	39.0
14	37.74353035158962	41.0	39.0	41.0	33.0	41.0
15	37.70871641734268	41.0	39.0	41.0	32.0	41.0
16	37.67110289012943	41.0	39.0	41.0	32.0	41.0
17	37.64951621953191	41.0	39.0	41.0	32.0	41.0
18	37.636510161577164	41.0	39.0	41.0	32.0	41.0
19	37.6103474051602	41.0	39.0	41.0	32.0	41.0
20	37.58373824309802	41.0	39.0	41.0	32.0	41.0
21	37.570900134092874	41.0	39.0	41.0	32.0	41.0
22	37.52099723255128	41.0	39.0	41.0	32.0	41.0
23	37.50439101863035	41.0	39.0	41.0	32.0	41.0
24	37.4466173407766	41.0	39.0	41.0	31.0	41.0
25	37.418038439957776	41.0	39.0	41.0	31.0	41.0
26	37.36080683968769	40.0	39.0	41.0	31.0	41.0
27	37.30398493594925	40.0	38.0	41.0	31.0	41.0
28	37.290813401679486	40.0	38.0	41.0	31.0	41.0
29	37.21174596532605	40.0	38.0	41.0	30.0	41.0
30	37.18364447318618	40.0	38.0	41.0	30.0	41.0
31	37.16839763768295	40.0	38.0	41.0	30.0	41.0
32	37.086500556342784	40.0	38.0	41.0	30.0	41.0
33	36.99016443020038	40.0	38.0	41.0	30.0	41.0
34	36.93601905830663	40.0	38.0	41.0	30.0	41.0
35	36.90442392369069	40.0	38.0	41.0	30.0	41.0
36	36.82622894694297	40.0	38.0	41.0	29.0	41.0
37	36.76110450685205	40.0	38.0	41.0	29.0	41.0
38	36.70127207539633	40.0	38.0	41.0	29.0	41.0
39	36.63879164249508	40.0	38.0	41.0	29.0	41.0
40	36.570423866629895	40.0	38.0	41.0	28.0	41.0
41	36.71564759250982	40.0	38.0	41.0	29.0	41.0
42	36.73557322326939	40.0	38.0	41.0	29.0	41.0
43	36.71018211904784	40.0	38.0	41.0	29.0	41.0
44	36.67295831708686	40.0	38.0	41.0	29.0	41.0
45	36.61598349040903	40.0	38.0	41.0	29.0	41.0
46	36.517117288470864	40.0	37.0	41.0	28.0	41.0
47	36.46701505454061	40.0	37.0	41.0	28.0	41.0
48	36.37564302764596	40.0	37.0	41.0	28.0	41.0
49	36.30734733858927	40.0	37.0	41.0	28.0	41.0
50	36.20352616713108	40.0	37.0	41.0	27.0	41.0
51	34.814867761599984	38.0	35.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1613.0
3	980.0
4	1759.0
5	2556.0
6	6034.0
7	15836.0
8	58088.0
9	76404.0
10	68089.0
11	41160.0
12	22879.0
13	14963.0
14	11944.0
15	10297.0
16	9382.0
17	8677.0
18	8453.0
19	8253.0
20	8380.0
21	8909.0
22	9059.0
23	9810.0
24	10465.0
25	11042.0
26	12561.0
27	14339.0
28	16774.0
29	19585.0
30	23341.0
31	29905.0
32	39281.0
33	53118.0
34	78569.0
35	121437.0
36	204204.0
37	389863.0
38	1060968.0
39	2768119.0
40	454.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.042847491878046	27.856494699670858	30.757955918819846	22.34270188963125
2	21.87784529511896	29.910617765908533	29.306006827185723	18.905530111786792
3	21.933097186332937	28.564840257888523	28.818614149199313	20.683448406579224
4	21.707815814687997	26.717950505229986	29.81610150269189	21.758132177390127
5	24.32599701977139	27.151672689013623	28.669151777425483	19.85317851378951
6	21.889896696878058	26.78113016360913	30.223717126476018	21.10525601303679
7	23.672925844175126	27.143398702701425	28.99229599128908	20.191379461834366
8	22.279553803318482	27.251082915161305	29.913040926389588	20.556322355130618
9	22.17727787883694	27.08488652272879	30.030533624129752	20.707301974304517
10	22.200547811517765	27.861114340882104	29.230700318127145	20.707637529472983
11	23.64323892174716	27.219267657161332	28.38458988499849	20.75290353609302
12	21.95918428063291	28.702537360883024	28.381340851439653	20.956937507044408
13	22.733655781650974	27.333161615294355	28.797248695419896	21.135933907634772
14	22.92641764477065	27.687032683121792	28.293745364289098	21.092804307818458
15	22.500384979630905	28.086816293826377	28.030104548446083	21.382694178096635
16	22.71349119427686	27.554064708200638	28.337218056675507	21.395226040847
17	22.725885287068667	27.66468997652979	28.274342829272577	21.33508190712897
18	22.480831360041883	27.507616036882855	28.326372198350658	21.685180404724605
19	22.380831449346207	27.621631736526947	28.531081968715633	21.466454845411217
20	22.377832337708963	27.723282531263532	28.48597208943307	21.41291304159443
21	22.290306183122716	27.617029342445864	28.43006013026283	21.66260434416859
22	22.23723273117038	27.643327384103195	28.547058932527403	21.572380952199023
23	22.45955082576421	27.67561028444854	28.354771980461134	21.510066909326117
24	22.18188312506924	27.7130425149083	28.381213063525962	21.7238612964965
25	22.285905490125828	27.755653094300172	28.2691384347769	21.689302980797105
26	22.30057494317422	27.81554065669589	28.27157947013543	21.612304929994462
27	22.30193528574956	27.92218370251711	28.075021183928055	21.700859827805267
28	22.291180996196974	27.872896285660865	28.088845127323093	21.747077590819067
29	22.371591547379136	27.790303331742216	28.064451052779543	21.773654068099102
30	22.18948358637853	27.787046448405846	28.11507282937154	21.908397135844083
31	22.15180109384368	27.76733004778995	28.253019345293644	21.82784951307272
32	22.402790510646202	27.84887201092337	27.9955050590023	21.752832419428124
33	22.283317480145314	27.742926231105102	28.000339984032212	21.973416304717375
34	22.22440746228263	27.744916354362097	28.1571813231928	21.873494860162474
35	22.404616078246924	27.810588175309686	27.942542689003837	21.84225305743955
36	22.30198417405219	27.72016237363623	27.994955285747764	21.982898166563814
37	22.397757208665016	27.660596262205544	28.00851939653319	21.933127132596248
38	22.447805150188827	27.772376711827224	28.053863062234235	21.725955075749713
39	22.270525953673268	27.648058252427184	27.995260301200215	22.086155492699337
40	22.250977342774544	27.84918452185708	27.967467273448772	21.932370861919605
41	22.354499021673664	27.76697538467532	27.960864654862583	21.917660938788433
42	22.16414421846007	27.69776318069374	28.023351089142466	22.114741511703723
43	22.203332232426	27.768208548545424	28.05712027106785	21.97133894796072
44	22.356104964562302	27.709048601332558	27.97478118695798	21.960065247147156
45	22.407174429913486	27.548726890715354	27.98914035381761	22.054958325553557
46	22.24945109535315	27.6436924084963	28.07310624312973	22.033750253020816
47	22.377836046177006	27.719503009026507	27.916646124954	21.98601481984249
48	22.312342395907216	27.624644643993985	27.842972315210098	22.220040644888705
49	22.116193489581246	27.825649814207875	28.002754710482375	22.05540198572851
50	22.231416271989563	27.77007499948379	28.05033844214899	21.948170286377653
51	22.3951245782743	27.31839369738293	28.037099426347822	22.249382297994945
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.38554938150929
2	40.78337540690575
3	42.61654559291216
4	43.46594799207813
5	44.1791755335609
6	42.99515270991485
7	43.86430530600949
8	42.835876158449096
9	42.88457985314146
10	42.90818534099075
11	44.396142457840185
12	42.91612178767732
13	43.869589689285746
14	44.01922195258911
15	43.883079157727536
16	44.10871723512386
17	44.06096719419764
18	44.16601176476649
19	43.847286294757424
20	43.790745379303395
21	43.95291052729131
22	43.809613683369406
23	43.969617735090324
24	43.90574442156574
25	43.97520847092294
26	43.912879873168684
27	44.00279511355483
28	44.038258587016045
29	44.14524561547824
30	44.09788072222261
31	43.9796506069164
32	44.15562293007433
33	44.25673378486269
34	44.0979023224451
35	44.24686913568648
36	44.28488234061601
37	44.33088434126126
38	44.17376022593854
39	44.356681446372605
40	44.18334820469415
41	44.27215996046209
42	44.27888573016379
43	44.174671180386724
44	44.31617021170946
45	44.462132755467046
46	44.28320134837397
47	44.3638508660195
48	44.53238304079592
49	44.17159547530975
50	44.17958655836722
51	44.64450687626924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3695.0
1	3382.5
2	3070.0
3	3376.5
4	3683.0
5	6002.5
6	8322.0
7	9149.5
8	9977.0
9	8131.0
10	6285.0
11	6539.5
12	6794.0
13	7603.5
14	8413.0
15	10127.0
16	11841.0
17	15000.5
18	18160.0
19	21515.0
20	24870.0
21	29056.5
22	33243.0
23	38780.0
24	44317.0
25	54132.0
26	76640.0
27	89333.0
28	96737.5
29	104142.0
30	121772.0
31	139402.0
32	153316.0
33	167230.0
34	202587.0
35	237944.0
36	270266.0
37	302588.0
38	345954.0
39	389320.0
40	414840.5
41	440361.0
42	478803.5
43	517246.0
44	533450.5
45	549655.0
46	529781.5
47	509908.0
48	446236.0
49	382564.0
50	343862.0
51	305160.0
52	267269.0
53	229378.0
54	198290.5
55	167203.0
56	148658.5
57	130114.0
58	111325.0
59	92536.0
60	88163.5
61	83791.0
62	67062.5
63	50334.0
64	42705.5
65	35077.0
66	29195.0
67	23313.0
68	19627.5
69	15942.0
70	13931.5
71	11921.0
72	10199.5
73	8478.0
74	7342.0
75	5276.0
76	4346.0
77	4018.0
78	3690.0
79	3594.5
80	3499.0
81	3121.0
82	2743.0
83	2475.0
84	2207.0
85	1954.5
86	1702.0
87	1951.5
88	2201.0
89	1481.5
90	762.0
91	521.5
92	281.0
93	210.0
94	139.0
95	111.0
96	83.0
97	68.5
98	54.0
99	67.0
100	80.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07982805679451455
2	0.06204410799707088
3	0.15206702741771355
4	0.1288242622514289
5	0.11935216973685463
6	0.10151116014113037
7	0.2054378940761381
8	0.18847181672071592
9	0.14455402231077213
10	0.21340738556932412
11	0.22099647174063963
12	0.2668733535582163
13	0.31193236393377144
14	0.3486795180264572
15	0.32359178704910085
16	0.4363819649836901
17	0.3612899544464627
18	0.3101634791870738
19	0.38858403629066773
20	0.35109509182033455
21	0.35676313111620434
22	0.43174102005687054
23	0.2555943357647574
24	0.422440109937138
25	0.41022909910509653
26	0.42320092058087894
27	0.40454203954313317
28	0.2080246502648572
29	0.34310658006105504
30	0.27919848598681896
31	0.1345493623455792
32	0.2893362878146665
33	0.4186360567184335
34	0.48459833953077003
35	0.3974665005563428
36	0.41789426634078614
37	0.4714172951279588
38	0.5154682314005573
39	0.4783596922520946
40	0.49486928322127227
41	0.40049072286521287
42	0.43782750520679786
43	0.4850548259170146
44	0.40815589010090253
45	0.47487898355698
46	0.4900951964317981
47	0.46211638500822627
48	0.5112647525938887
49	0.37994883548420844
50	0.5157154948597731
51	0.5804034198438436
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5257550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	55.826023559522085
#Duplication Level	Relative count
1	100.0
2	3.046391246890996
3	0.8379162891619106
4	0.4841678833380461
5	0.34740055514839546
6	0.2856532803961059
7	0.24525786700675759
8	0.22794554698275116
9	0.19389798426887186
10++	9.746836173515613
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1919495	4.7919655	5.183801	36
TTTTT	1576240	3.4364247	4.1588707	16
TTTGA	809845	2.3118582	14.159072	8
TGTTT	823010	2.2866302	13.854864	6
GTTTG	596840	2.1132705	16.808659	7
CTTTG	559390	2.0539794	17.549608	1
TGTGT	565400	2.0019488	16.42838	4
GAGGG	314190	1.8563961	5.5954585	11
GTGTT	485845	1.7202634	16.505001	5
TTGAG	460090	1.6738187	10.574535	9
TGAGG	356915	1.6547667	6.5707917	10
TTTGT	574555	1.5963291	13.246814	2
TTGTG	406095	1.4378873	16.25777	3
ACTTT	415490	1.2299972	6.298622	3
AACTT	386690	1.176183	6.595714	2
TAACT	248265	0.75513995	6.2110186	1
>>END_MODULE