FastQCFastQC Report
Tue 12 Aug 2014
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane5_Undetermined_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5257550
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1283110 3.5730026 4.3479757 17
TGTTT 637750 2.1441586 18.629736 6
TGTGT 506690 2.0567703 21.665548 4
GTTTG 483660 1.9632864 21.702444 7
TTTGA 552955 1.7538348 17.268574 8
TTTGT 508465 1.7094939 18.184303 2
CTTTG 406510 1.6983 22.213573 1
GTGTT 395425 1.6051203 21.5672 5
GAGGG 278075 1.5522865 5.451265 11
TTGAG 384440 1.4721922 12.306842 9
TGAGG 308340 1.4256166 7.014454 10
TTGTG 351030 1.4249109 21.39184 3
Produced by FastQC (version 0.10.1)