FastQCFastQC Report
Tue 12 Aug 2014
lane4_Undetermined_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane4_Undetermined_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4745090
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1462785 4.0935307 4.3648357 7
TTTTT 1264510 3.0395012 3.636315 18
TGTTT 739845 2.2673419 14.2318945 6
TTTGA 703550 2.2226892 14.48949 8
TGTGT 544340 2.126879 17.02985 4
GTTTG 530975 2.0746584 17.371693 7
CTTTG 502590 2.0384471 18.1619 1
GAGGG 287740 1.8839645 5.671446 11
GTGTT 434820 1.6989555 16.966585 5
TGAGG 324185 1.6648272 6.945587 10
TTGAG 407880 1.6429051 10.907573 9
TTTGT 530260 1.6250441 13.657829 2
TTGTG 375525 1.4672744 16.829397 3
ACTTT 371665 1.218845 5.5829487 3
AACTT 340555 1.1513082 5.842367 2
TAACT 225830 0.76345944 5.499479 1