##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane4_Undetermined_L004_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4745090	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.128184502296058	33.0	31.0	34.0	27.0	34.0
2	31.125516059758613	34.0	31.0	34.0	27.0	34.0
3	31.201361407265193	34.0	31.0	34.0	27.0	34.0
4	34.51784286494039	37.0	35.0	37.0	31.0	37.0
5	34.56804043758917	37.0	35.0	37.0	31.0	37.0
6	34.59829529050029	37.0	35.0	37.0	31.0	37.0
7	34.57471639104843	37.0	35.0	37.0	31.0	37.0
8	34.60158037044608	37.0	35.0	37.0	32.0	37.0
9	36.24910086004691	39.0	38.0	39.0	32.0	39.0
10	36.20997325656626	39.0	38.0	39.0	32.0	39.0
11	36.22403242088137	39.0	38.0	39.0	32.0	39.0
12	36.15346705752683	39.0	38.0	39.0	31.0	39.0
13	36.12879207770558	39.0	37.0	39.0	31.0	39.0
14	37.55398232699485	41.0	38.0	41.0	31.0	41.0
15	37.51927297480132	41.0	38.0	41.0	31.0	41.0
16	37.46866550476387	40.0	38.0	41.0	31.0	41.0
17	37.44021462185122	40.0	38.0	41.0	31.0	41.0
18	37.41683318967606	40.0	38.0	41.0	31.0	41.0
19	37.437742171381366	40.0	39.0	41.0	31.0	41.0
20	37.40139575856306	40.0	38.0	41.0	31.0	41.0
21	37.364092567264265	40.0	38.0	41.0	31.0	41.0
22	37.336883388934666	40.0	38.0	41.0	31.0	41.0
23	37.30677142056315	40.0	38.0	41.0	31.0	41.0
24	37.25755317601984	40.0	38.0	41.0	30.0	41.0
25	37.19617773319368	40.0	38.0	41.0	30.0	41.0
26	37.14453909198772	40.0	38.0	41.0	30.0	41.0
27	37.09873679951276	40.0	38.0	41.0	30.0	41.0
28	37.06012636219756	40.0	38.0	41.0	30.0	41.0
29	36.99846746847794	40.0	38.0	41.0	30.0	41.0
30	36.96881576534902	40.0	38.0	41.0	30.0	41.0
31	36.910983353318905	40.0	38.0	41.0	29.0	41.0
32	36.847623543494436	40.0	38.0	41.0	29.0	41.0
33	36.74572157746218	40.0	38.0	41.0	29.0	41.0
34	36.68382306763412	40.0	38.0	41.0	29.0	41.0
35	36.6297130296791	40.0	38.0	41.0	28.0	41.0
36	36.57766975968843	40.0	38.0	41.0	28.0	41.0
37	36.51029969926809	40.0	37.0	41.0	28.0	41.0
38	36.44844102008602	40.0	37.0	41.0	27.0	41.0
39	36.35446851376897	40.0	37.0	41.0	27.0	41.0
40	36.28283193785576	40.0	37.0	41.0	27.0	41.0
41	36.451144024665496	40.0	37.0	41.0	28.0	41.0
42	36.487319523971095	40.0	37.0	41.0	28.0	41.0
43	36.465976409298875	40.0	37.0	41.0	28.0	41.0
44	36.41711664056952	40.0	37.0	41.0	27.0	41.0
45	36.34158635558019	40.0	37.0	41.0	27.0	41.0
46	36.28758253268115	40.0	37.0	41.0	27.0	41.0
47	36.22663405752051	40.0	37.0	41.0	27.0	41.0
48	36.153997922062594	40.0	36.0	41.0	27.0	41.0
49	36.0679999747107	40.0	36.0	41.0	27.0	41.0
50	35.97234404405396	40.0	36.0	41.0	26.0	41.0
51	34.60953111532131	38.0	34.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	678.0
3	2327.0
4	2962.0
5	3199.0
6	4049.0
7	13516.0
8	38247.0
9	52713.0
10	53917.0
11	34255.0
12	20456.0
13	15715.0
14	13788.0
15	12934.0
16	11899.0
17	11627.0
18	11443.0
19	11316.0
20	11396.0
21	12088.0
22	12971.0
23	13540.0
24	14434.0
25	16034.0
26	17134.0
27	18955.0
28	21991.0
29	25787.0
30	30328.0
31	37175.0
32	47971.0
33	63997.0
34	89993.0
35	134677.0
36	215840.0
37	399242.0
38	1023140.0
39	2223147.0
40	209.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.158197323318696	28.020075636636456	30.355854673971113	22.465872366073732
2	21.917200145136256	29.691488357965618	29.497867809913718	18.89344368698441
3	21.90512192106952	28.315275562247734	29.18477822925135	20.594824287431397
4	21.74647739984781	26.64536346349505	30.010731043931475	21.597428092725668
5	24.36599122271435	27.07010022027546	28.72281976204853	19.84108879496166
6	21.976721928826745	26.7566064575862	30.238830851581426	21.027840762005628
7	23.74358198791297	27.09140381367424	28.928241618880975	20.236772579531813
8	22.199064804151963	27.158510252678425	30.052460765196297	20.589964177973314
9	22.155716172788726	26.971524713032174	30.162724668965264	20.710034445213832
10	22.113523487210003	27.78919071060052	29.440048786589944	20.657237015599538
11	23.69699912645153	27.148635518979496	28.428572092683197	20.725793261885777
12	21.887133310848466	28.76627490244686	28.41520631415645	20.93138547254822
13	22.769428500489273	27.32769574551987	28.827517626193256	21.0753581277976
14	22.942778749548737	27.595922822434805	28.410651550208627	21.050646877807832
15	22.5227061890481	27.94812094668433	28.144094273819004	21.38507859044856
16	22.70884036463854	27.455925376298495	28.42552469790121	21.409709561161755
17	22.760299182324882	27.52152541086256	28.36061673594068	21.357558670871875
18	22.503305546108447	27.427805940059997	28.41902450980808	21.649864004023478
19	22.362816899498572	27.55109737581557	28.610759041755497	21.475326682930366
20	22.38033709246932	27.63734614102654	28.52316164341272	21.45915512309142
21	22.261315799514175	27.5983415788068	28.466426721269084	21.67391590040994
22	22.21853033898057	27.632997327222515	28.559579967285863	21.58889236651105
23	22.417546743042845	27.656196162910796	28.43689620346939	21.489360890576968
24	22.221251214349046	27.701475884830923	28.34797622633345	21.72929667448658
25	22.268748839366648	27.75656250715459	28.298466988831635	21.67622166464713
26	22.294853126891113	27.725255156959033	28.28216350725763	21.697728208892226
27	22.303975985344547	27.855244522282284	28.108500235485618	21.73227925688756
28	22.281420429160388	27.776418983704925	28.14115275285085	21.80100783428383
29	22.403077858764767	27.734402234435613	28.078277297612882	21.784242609186744
30	22.243213128467733	27.765423130101848	28.112905533725012	21.878458207705407
31	22.155016455638584	27.769411460830923	28.244290809083893	21.831281274446603
32	22.49149722931522	27.738210021084754	28.044119049384943	21.726173700215085
33	22.329687226207845	27.685332229078092	28.056384901968357	21.92859564274571
34	22.270321300474937	27.667693005156877	28.154367195145735	21.907618499222444
35	22.421730605533458	27.738550734110817	28.021677295142833	21.81804136521289
36	22.35491853027686	27.682855918067645	27.931191083532465	22.031034468123035
37	22.376799586410286	27.63686788983505	28.027727998546663	21.958604525208006
38	22.51046033986817	27.690764544171188	28.070924394722596	21.727850721238045
39	22.341011035268245	27.66394508239572	27.956642566704353	22.03840131563168
40	22.219436750788375	27.79100699816088	28.01380697574503	21.975749275305713
41	22.365217797342243	27.686230895109613	28.022939664522262	21.92561164302588
42	22.217699438830582	27.645350774393425	28.041747507293163	22.095202279482827
43	22.265145650378383	27.713701606865506	28.070697921639542	21.950454821116573
44	22.422148589216754	27.635884622885992	27.973217921980186	21.96874886591707
45	22.41089628812712	27.567900165468316	27.980021547387697	22.041181999016867
46	22.25728062076788	27.581708559483353	28.100840814231365	22.0601700055174
47	22.35720153427821	27.652074608205034	28.029276100029506	21.96144775748725
48	22.36779412701446	27.535150858865748	27.88338960018685	22.213665413932947
49	22.221776558782157	27.740129828126708	28.009628876953595	22.028464736137533
50	22.26714269486741	27.682207649785923	28.054558522061956	21.99609113328471
51	22.381004066060363	27.34052294425846	28.079308461751356	22.199164527929817
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.62406968939243
2	40.81064383212066
3	42.49994620850091
4	43.34390549257348
5	44.207080017676006
6	43.00456269083237
7	43.980354567444785
8	42.789028982125274
9	42.86575061800256
10	42.77076050280954
11	44.42279238833731
12	42.81851878339669
13	43.844786628286876
14	43.99342562735657
15	43.90778477949666
16	44.1185499258003
17	44.11785785319676
18	44.15316955013193
19	43.838143582428934
20	43.83949221556074
21	43.93523169992412
22	43.807422705491625
23	43.90690763361981
24	43.95054788883562
25	43.944970504013774
26	43.992581335783335
27	44.0362552422321
28	44.082428263444214
29	44.18732046795151
30	44.121671336173144
31	43.986297730085184
32	44.21767092953031
33	44.25828286895356
34	44.177939799697384
35	44.23977197074635
36	44.385952998399894
37	44.33540411161829
38	44.23831106110622
39	44.37941235089992
40	44.19518602609409
41	44.29082944036812
42	44.312901718313405
43	44.21560047149495
44	44.39089745513382
45	44.45207828714398
46	44.31745062628528
47	44.31864929176546
48	44.58145954094741
49	44.25024129491969
50	44.263233828152124
51	44.58016859399018
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2332.0
1	2663.0
2	2994.0
3	3865.0
4	4736.0
5	6029.0
6	7322.0
7	7975.5
8	8629.0
9	7648.5
10	6668.0
11	6657.0
12	6646.0
13	7249.0
14	7852.0
15	9225.5
16	10599.0
17	13420.5
18	16242.0
19	19740.0
20	23238.0
21	27750.0
22	32262.0
23	37973.5
24	43685.0
25	53168.0
26	74108.5
27	85566.0
28	93639.0
29	101712.0
30	117665.5
31	133619.0
32	146732.0
33	159845.0
34	190006.5
35	220168.0
36	247582.5
37	274997.0
38	310202.0
39	345407.0
40	366669.5
41	387932.0
42	418692.5
43	449453.0
44	461375.0
45	473297.0
46	457205.5
47	441114.0
48	390997.5
49	340881.0
50	308488.5
51	276096.0
52	243355.5
53	210615.0
54	183432.5
55	156250.0
56	139331.5
57	122413.0
58	104869.5
59	87326.0
60	82003.5
61	76681.0
62	61861.0
63	47041.0
64	40134.0
65	33227.0
66	27594.0
67	21961.0
68	18818.5
69	15676.0
70	13871.0
71	12066.0
72	10377.5
73	8689.0
74	7321.5
75	5153.5
76	4353.0
77	3979.5
78	3606.0
79	3487.0
80	3368.0
81	2943.5
82	2519.0
83	2335.5
84	2152.0
85	1905.5
86	1659.0
87	1858.5
88	2058.0
89	1444.5
90	831.0
91	559.5
92	288.0
93	224.0
94	160.0
95	128.5
96	97.0
97	77.0
98	57.0
99	78.5
100	100.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.376045554457344E-4
2	0.15748910979559924
3	0.09618363403012377
4	0.07810178521376834
5	0.04084221795582381
6	0.029462033386089624
7	0.2267185659281489
8	0.11692085924608385
9	0.14417007896583625
10	0.2664227654269993
11	0.2669707002396161
12	0.39931803190245074
13	0.4368515665667037
14	0.4102767281547874
15	0.45284704821194116
16	0.5919803417848766
17	0.6119167391977813
18	0.6063952422398733
19	0.5347843771140274
20	0.6060580515859552
21	0.5763852740411668
22	0.5485459706770578
23	0.5477662172898723
24	0.645509358094367
25	0.5866695889856672
26	0.5403901717354149
27	0.6175436082350387
28	0.5659323637697072
29	0.5526765561875538
30	0.4388114872426023
31	0.459106149725295
32	0.5487777892516265
33	0.657606072803677
34	0.7063933455424449
35	0.6967834119057805
36	0.6935590262776892
37	0.6991226720673369
38	0.7258028825586027
39	0.7110718658655579
40	0.7194384089658995
41	0.7081003732279051
42	0.6984904395912406
43	0.718995846232632
44	0.698258621016672
45	0.7083954150500833
46	0.7278471008979809
47	0.7182160928454465
48	0.7458235776349869
49	0.6957507655281565
50	0.7436107639686498
51	0.7817133078613894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4745090.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	49.4384069496417
#Duplication Level	Relative count
1	100.0
2	2.8385465817905207
3	0.7046321557054043
4	0.4272404385648839
5	0.33287006056862445
6	0.2487946328992296
7	0.20990259833107416
8	0.1704386220780929
9	0.1767299806111769
10++	9.279181894614025
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1462785	4.0935307	4.3648357	7
TTTTT	1264510	3.0395012	3.636315	18
TGTTT	739845	2.2673419	14.2318945	6
TTTGA	703550	2.2226892	14.48949	8
TGTGT	544340	2.126879	17.02985	4
GTTTG	530975	2.0746584	17.371693	7
CTTTG	502590	2.0384471	18.1619	1
GAGGG	287740	1.8839645	5.671446	11
GTGTT	434820	1.6989555	16.966585	5
TGAGG	324185	1.6648272	6.945587	10
TTGAG	407880	1.6429051	10.907573	9
TTTGT	530260	1.6250441	13.657829	2
TTGTG	375525	1.4672744	16.829397	3
ACTTT	371665	1.218845	5.5829487	3
AACTT	340555	1.1513082	5.842367	2
TAACT	225830	0.76345944	5.499479	1
>>END_MODULE