FastQCFastQC Report
Tue 12 Aug 2014
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane4_Undetermined_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4745090
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1067820 3.1319637 3.8066976 16
TGTGT 504455 2.2378025 23.238594 4
TGTTT 597200 2.1541643 19.441242 6
GTTTG 439570 1.9499674 23.158655 7
CTTTG 388770 1.7677084 23.661959 1
TTTGA 510265 1.7554059 18.176409 8
TTTGT 486160 1.7536311 19.119055 2
GTGTT 365620 1.6219193 22.972586 5
GAGGG 251035 1.6063385 5.9333687 11
TGAGG 288000 1.4984937 7.8178697 10
TTGTG 337140 1.4955798 22.87028 3
TTGAG 351800 1.488394 13.424723 9
Produced by FastQC (version 0.10.1)