##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane4_Undetermined_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4745090	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46870702136314	34.0	31.0	34.0	31.0	34.0
2	32.78042144616857	34.0	31.0	34.0	31.0	34.0
3	32.906813147906576	34.0	31.0	34.0	31.0	34.0
4	36.39957872242676	37.0	37.0	37.0	35.0	37.0
5	36.3238077676082	37.0	37.0	37.0	35.0	37.0
6	36.3211936970637	37.0	37.0	37.0	35.0	37.0
7	36.31896212716724	37.0	37.0	37.0	35.0	37.0
8	36.326100874799	37.0	37.0	37.0	35.0	37.0
9	38.07425991920069	39.0	38.0	39.0	37.0	39.0
10	38.06777742887912	39.0	38.0	39.0	37.0	39.0
11	38.06352882663975	39.0	38.0	39.0	37.0	39.0
12	38.04845513994466	39.0	38.0	39.0	37.0	39.0
13	38.020979580998464	39.0	38.0	39.0	37.0	39.0
14	39.53752636936286	41.0	40.0	41.0	37.0	41.0
15	39.564499092746395	41.0	40.0	41.0	37.0	41.0
16	39.54557047390039	41.0	40.0	41.0	37.0	41.0
17	39.52944074822606	41.0	40.0	41.0	37.0	41.0
18	39.50054962076589	41.0	39.0	41.0	37.0	41.0
19	39.52565578313583	41.0	40.0	41.0	37.0	41.0
20	39.494699995152885	41.0	39.0	41.0	37.0	41.0
21	39.456591128935386	41.0	39.0	41.0	37.0	41.0
22	39.42242107104396	41.0	39.0	41.0	37.0	41.0
23	39.38349620344398	41.0	39.0	41.0	37.0	41.0
24	39.33386089621061	41.0	39.0	41.0	36.0	41.0
25	39.24702460859541	40.0	39.0	41.0	36.0	41.0
26	39.11046407971187	40.0	39.0	41.0	36.0	41.0
27	39.05839615265464	40.0	39.0	41.0	36.0	41.0
28	38.984948441441574	40.0	39.0	41.0	36.0	41.0
29	38.91898488753638	40.0	39.0	41.0	36.0	41.0
30	38.86592688442158	40.0	39.0	41.0	36.0	41.0
31	38.76373535591527	40.0	38.0	41.0	35.0	41.0
32	38.639139405153536	40.0	38.0	41.0	35.0	41.0
33	38.53269168761815	40.0	38.0	41.0	35.0	41.0
34	38.636428603040194	40.0	38.0	41.0	35.0	41.0
35	38.608096579833045	40.0	39.0	41.0	35.0	41.0
36	38.58640847697304	40.0	38.0	41.0	35.0	41.0
37	38.55580357801433	40.0	38.0	41.0	35.0	41.0
38	38.51316919173293	40.0	38.0	41.0	35.0	41.0
39	38.283830443679676	40.0	38.0	41.0	34.0	41.0
40	38.221789260056184	40.0	38.0	41.0	34.0	41.0
41	38.24033263858009	40.0	38.0	41.0	34.0	41.0
42	38.00844725811312	40.0	38.0	41.0	34.0	41.0
43	38.01620685803641	40.0	38.0	41.0	34.0	41.0
44	37.99535667395139	40.0	38.0	41.0	34.0	41.0
45	38.005768067623585	40.0	38.0	41.0	34.0	41.0
46	37.973948017845814	40.0	38.0	41.0	34.0	41.0
47	37.894562379217255	40.0	38.0	41.0	34.0	41.0
48	37.824142640076374	40.0	38.0	41.0	33.0	41.0
49	37.676798543336375	40.0	37.0	41.0	33.0	41.0
50	37.481629010197906	40.0	37.0	41.0	33.0	41.0
51	37.3261554575361	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	7.0
12	4.0
13	21.0
14	33.0
15	108.0
16	294.0
17	671.0
18	1811.0
19	5002.0
20	3735.0
21	4904.0
22	6291.0
23	9837.0
24	13096.0
25	16357.0
26	19116.0
27	22041.0
28	24618.0
29	28018.0
30	31743.0
31	37836.0
32	46240.0
33	60908.0
34	86537.0
35	132516.0
36	220943.0
37	433183.0
38	1099384.0
39	2438966.0
40	861.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.663478328144986	30.39009486594791	27.991852671818968	23.95457413408814
2	20.14364321856909	30.06598399608859	29.92735648849653	19.863016296845792
3	19.21264067665582	28.620984136980148	30.672052834861972	21.494322351502063
4	19.983877441572588	28.487599458983603	30.129052141972956	21.39947095747085
5	24.18976247025873	28.212383748253455	27.59302774025361	20.004826041234203
6	22.26322366909795	27.708599836884023	28.842361261851725	21.185815232166302
7	24.122914423119475	28.434065528788704	26.802737145133182	20.64028290295864
8	21.81062951387645	28.171878720951554	28.970072222023184	21.047419543148813
9	21.510276938898944	27.94204114147466	29.345534015160936	21.202147904465455
10	21.625782967895628	28.45529037829873	28.758256436237406	21.16067021756824
11	23.77322664059059	28.187789904933307	26.95790806918309	21.08107538529301
12	21.707638843520353	30.135044856894176	26.87685586574754	21.280460433837924
13	22.81143596102925	28.641178766555697	27.17990209260822	21.367483179806833
14	22.53459892225437	28.726367676903912	27.425865473573737	21.313167927267976
15	22.50383027508435	28.36624805851944	27.457182055556373	21.672739610839837
16	22.394496205551423	28.621037746386264	27.3704819086677	21.613984139394617
17	22.60323570426477	28.602748735937734	27.221613804603432	21.572401755194065
18	22.302232412873096	28.379651387012682	27.39773112838745	21.920385071726773
19	22.201728523589647	28.722342463472767	27.39060797582343	21.685321037114154
20	22.281474113241266	28.679582473672788	27.39170384544866	21.647239567637282
21	22.290789005055753	28.472652784246456	27.24205441835666	21.994503792341135
22	22.187320272815462	28.75146020928384	27.285002293974824	21.776217223925872
23	22.430385935777824	28.62969933130878	27.275984227907163	21.663930505006228
24	22.214183503368744	28.514864839233816	27.258408165071685	22.01254349232575
25	22.221411012523344	28.837913971914798	27.112984395935978	21.827690619625884
26	22.45038216643941	28.60413016095822	27.109795790570512	21.83569188203186
27	22.467365390480794	28.509810171001238	27.01986307658698	22.002961361930986
28	22.390741192632742	28.828987242479027	26.86400055390388	21.916271010984353
29	22.586235106716504	28.794297193248763	26.866274755284103	21.75319294475063
30	22.40415444962021	28.60455071007562	27.01267535706256	21.97861948324161
31	22.323068134475736	28.777957681195183	26.921788209922497	21.97718597440658
32	22.556915999456095	28.8138535901642	26.89548882404229	21.73374158633741
33	22.467173457950487	28.574876371002695	26.912592781238388	22.04535738980843
34	22.340870560778527	28.787342471043125	26.888442146373848	21.983344821804497
35	22.621747024580944	28.636632120432072	26.928874996264636	21.812745858722355
36	22.65590388108931	28.440102681722696	26.797640270567612	22.106353166620384
37	22.563736637762233	28.68221241020447	26.779480160739745	21.974570791293555
38	22.630604406197236	28.63712535777313	26.917081376306363	21.81518885972327
39	22.706780409929454	28.501558354932477	26.78937830018267	22.0022829349554
40	22.549036197483275	28.902251650474263	26.59596609960125	21.952746052441213
41	22.532624564834496	28.862331616089236	26.865365138482606	21.73967868059366
42	22.59629101888661	28.67937870771986	26.761189454972335	21.9631408184212
43	22.50836444448025	28.95396370039928	26.608272621632413	21.929399233488052
44	22.663847914479962	28.838270915836734	26.701223212763185	21.796657956920118
45	22.635557663077762	28.558094931644305	26.72335101676186	22.082996388516072
46	22.56897443463968	28.747010711199533	26.670305111062458	22.013709743098325
47	22.64491438102039	28.67297409275088	26.768128310228995	21.91398321599974
48	22.51570997925664	28.603618102029525	26.68369304576667	22.196978872947156
49	22.364528245338587	28.975637921889465	26.593910475257577	22.065923357514375
50	22.53690243125885	28.99353657049567	26.600234387172385	21.8693266110731
51	22.485907784385653	28.511946477269667	26.803100234391096	22.199045503953585
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.61805246223312
2	40.00665951541488
3	40.70696302815789
4	41.38334839904344
5	44.19458851149293
6	43.44903890126425
7	44.763197326078114
8	42.85804905702526
9	42.7124248433644
10	42.78645318546387
11	44.8543020258836
12	42.98809927735828
13	44.17891914083608
14	43.847766849522344
15	44.17656988592419
16	44.00848034494604
17	44.175637459458834
18	44.222617484599866
19	43.8870495607038
20	43.92871368087855
21	44.285292797396885
22	43.96353749674133
23	44.094316440784056
24	44.2267269956945
25	44.049101632149224
26	44.28607404847127
27	44.47032675241178
28	44.30701220361709
29	44.33942805146713
30	44.38277393286182
31	44.30025410888232
32	44.29065758579351
33	44.51253084775892
34	44.32421538258303
35	44.4344928833033
36	44.76225704770969
37	44.538307429055784
38	44.44579326592051
39	44.709063344884854
40	44.50178224992449
41	44.272303245428155
42	44.559431837307805
43	44.437763677968306
44	44.46050587140008
45	44.71855405159384
46	44.58268417773801
47	44.55889759702013
48	44.712688852203804
49	44.43045160285296
50	44.40622904233195
51	44.684953288339244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2207.0
1	1389.5
2	572.0
3	720.5
4	869.0
5	1367.5
6	1866.0
7	3067.5
8	4269.0
9	3495.5
10	2722.0
11	3015.0
12	3308.0
13	4291.0
14	5274.0
15	7135.0
16	8996.0
17	12224.5
18	15453.0
19	19320.0
20	23187.0
21	27893.5
22	32600.0
23	38440.5
24	44281.0
25	54155.0
26	77545.0
27	91061.0
28	96066.0
29	101071.0
30	113384.0
31	125697.0
32	143243.0
33	160789.0
34	186314.5
35	211840.0
36	245261.5
37	278683.0
38	302181.0
39	325679.0
40	355282.5
41	384886.0
42	419542.0
43	454198.0
44	464001.5
45	473805.0
46	451582.0
47	429359.0
48	402706.0
49	376053.0
50	341976.5
51	307900.0
52	270534.0
53	233168.0
54	201144.5
55	169121.0
56	145649.5
57	122178.0
58	101781.0
59	81384.0
60	72337.0
61	63290.0
62	51565.5
63	39841.0
64	33926.5
65	28012.0
66	23269.0
67	18526.0
68	16623.5
69	14721.0
70	13095.5
71	11470.0
72	9833.0
73	8196.0
74	6840.0
75	4596.5
76	3709.0
77	3478.5
78	3248.0
79	3227.0
80	3206.0
81	2835.0
82	2464.0
83	2143.0
84	1822.0
85	1677.0
86	1532.0
87	1833.0
88	2134.0
89	1339.5
90	545.0
91	316.5
92	88.0
93	70.0
94	52.0
95	39.5
96	27.0
97	37.5
98	48.0
99	109.0
100	170.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030536828595453407
2	0.0
3	0.007249599059238075
4	0.030052117030446208
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	8.429766347951251E-5
11	0.0
12	0.0
13	0.004172734342235869
14	0.0
15	0.0
16	0.0
17	0.030663275090672674
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0010747952093637845
23	0.0
24	0.0
25	0.12518203026707608
26	0.24893100025500042
27	0.15605604951644753
28	0.16450689028026866
29	0.17860567449721712
30	0.07932410133422127
31	0.24627562385539578
32	0.34399769024402066
33	0.3674745895230649
34	0.45339498302455805
35	0.5622443409924786
36	0.5816538780086363
37	0.4988103492241454
38	0.525174443477363
39	0.412932104554392
40	0.36580971066934453
41	0.5581137554819825
42	0.5809794967008003
43	0.5984923362886689
44	0.5126351660347854
45	0.4482528255523078
46	0.37501923040448126
47	0.45653507098916984
48	0.47657684048142396
49	0.43101395337074744
50	0.4485057185427463
51	0.6749503170645867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4745090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	50.92776494921305
#Duplication Level	Relative count
1	100.0
2	3.272555110336147
3	0.7540502777168328
4	0.447464751325073
5	0.31986512546334256
6	0.2725204679037864
7	0.22690793196226283
8	0.2084319680365824
9	0.16686104920380143
10++	9.806117853554891
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1067820	3.1319637	3.8066976	16
TGTGT	504455	2.2378025	23.238594	4
TGTTT	597200	2.1541643	19.441242	6
GTTTG	439570	1.9499674	23.158655	7
CTTTG	388770	1.7677084	23.661959	1
TTTGA	510265	1.7554059	18.176409	8
TTTGT	486160	1.7536311	19.119055	2
GTGTT	365620	1.6219193	22.972586	5
GAGGG	251035	1.6063385	5.9333687	11
TGAGG	288000	1.4984937	7.8178697	10
TTGTG	337140	1.4955798	22.87028	3
TTGAG	351800	1.488394	13.424723	9
>>END_MODULE