FastQCFastQC Report
Tue 12 Aug 2014
lane3_Undetermined_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane3_Undetermined_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5597930
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1861860 4.233853 4.626218 36
TTTGA 768085 2.1028535 11.051119 8
TGTTT 776910 2.0993612 10.875023 6
CTTTG 535645 1.8923075 13.725135 1
GTTTG 546130 1.8722193 13.0816145 7
TGTGT 459335 1.5746725 12.569287 4
TGAGG 347425 1.5309091 5.3101997 10
TTGAG 434880 1.5104748 8.140751 9
TTTGT 546170 1.475857 10.395703 2
GTGTT 418215 1.4337066 12.633729 5
TTGTG 342255 1.173304 12.405915 3
Produced by FastQC (version 0.10.1)