##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane3_Undetermined_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5597930	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.827167899562873	33.0	31.0	34.0	26.0	34.0
2	30.807214988397497	34.0	31.0	34.0	26.0	34.0
3	30.847409846139556	34.0	31.0	34.0	26.0	34.0
4	34.1106096360619	37.0	35.0	37.0	28.0	37.0
5	34.131791394318974	37.0	35.0	37.0	28.0	37.0
6	34.12063494899007	37.0	35.0	37.0	29.0	37.0
7	34.11373311206106	37.0	35.0	37.0	29.0	37.0
8	34.16628039293096	37.0	35.0	37.0	29.0	37.0
9	35.74069146988262	39.0	37.0	39.0	29.0	39.0
10	35.74462828224004	39.0	37.0	39.0	29.0	39.0
11	35.719387344965014	39.0	37.0	39.0	29.0	39.0
12	35.67820855208979	39.0	37.0	39.0	29.0	39.0
13	35.671261877158166	39.0	37.0	39.0	28.0	39.0
14	37.05545281916709	40.0	38.0	41.0	28.0	41.0
15	37.03078566541561	40.0	38.0	41.0	28.0	41.0
16	36.973272620415045	40.0	38.0	41.0	28.0	41.0
17	36.988240831878926	40.0	38.0	41.0	28.0	41.0
18	36.97514974285138	40.0	38.0	41.0	27.0	41.0
19	36.953321852899194	40.0	38.0	41.0	27.0	41.0
20	36.960941097870105	40.0	38.0	41.0	27.0	41.0
21	36.96205490243715	40.0	38.0	41.0	27.0	41.0
22	36.8591382886174	40.0	38.0	41.0	27.0	41.0
23	36.84596806319479	40.0	38.0	41.0	27.0	41.0
24	36.754473528607896	40.0	38.0	41.0	27.0	41.0
25	36.73736005987928	40.0	38.0	41.0	27.0	41.0
26	36.675513448721226	40.0	38.0	41.0	27.0	41.0
27	36.633410028349765	40.0	38.0	41.0	27.0	41.0
28	36.59464462756769	40.0	38.0	41.0	27.0	41.0
29	36.50076849835564	40.0	38.0	41.0	26.0	41.0
30	36.488301747253004	40.0	38.0	41.0	26.0	41.0
31	36.44551736088161	40.0	38.0	41.0	26.0	41.0
32	36.38242189523628	40.0	38.0	41.0	26.0	41.0
33	36.333437717156166	40.0	37.0	41.0	25.0	41.0
34	36.23527107341464	40.0	37.0	41.0	25.0	41.0
35	36.2071487138996	40.0	37.0	41.0	25.0	41.0
36	36.159144898203444	40.0	37.0	41.0	25.0	41.0
37	36.09361246032015	40.0	37.0	41.0	25.0	41.0
38	35.96200988579707	40.0	37.0	41.0	24.0	41.0
39	35.90798723813981	40.0	37.0	41.0	24.0	41.0
40	35.799605568486925	40.0	36.0	41.0	24.0	41.0
41	36.0036129426413	40.0	37.0	41.0	24.0	41.0
42	36.01551287708135	40.0	37.0	41.0	24.0	41.0
43	35.97346894298428	40.0	37.0	41.0	24.0	41.0
44	35.93927148070805	40.0	37.0	41.0	24.0	41.0
45	35.85623364350751	40.0	36.0	41.0	24.0	41.0
46	35.79330895527454	40.0	36.0	41.0	24.0	41.0
47	35.74148765704466	40.0	36.0	41.0	23.0	41.0
48	35.63007040102323	40.0	36.0	41.0	23.0	41.0
49	35.56367639466731	40.0	36.0	41.0	23.0	41.0
50	35.45633493094769	39.0	35.0	41.0	23.0	41.0
51	34.11566311118574	38.0	34.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2941.0
3	3239.0
4	4429.0
5	5078.0
6	7660.0
7	16807.0
8	53932.0
9	79598.0
10	81893.0
11	52887.0
12	31817.0
13	23720.0
14	19309.0
15	16712.0
16	15001.0
17	14032.0
18	13657.0
19	13431.0
20	13492.0
21	14148.0
22	14685.0
23	15714.0
24	16595.0
25	17866.0
26	19418.0
27	21843.0
28	25438.0
29	30018.0
30	35725.0
31	44231.0
32	57054.0
33	76854.0
34	108986.0
35	163226.0
36	264951.0
37	495272.0
38	1241703.0
39	2464357.0
40	211.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.30233181309052	28.510787292842355	29.783414526089764	22.403466367977362
2	22.111501767612364	30.077418667116902	28.855453557205863	18.955626008064876
3	21.759907785002337	28.643729473117425	28.753992753514375	20.842369988365867
4	21.835435282441605	27.151752669750557	29.349608926734966	21.663203121072875
5	23.84734387177071	27.636474911486253	28.484327899479524	20.031853317263515
6	22.183400601830737	27.301077443395492	29.24035364010063	21.275168314673138
7	23.443626703046185	27.656662298730904	28.414834227862645	20.484876770360266
8	22.102382176944673	27.6694894076006	29.364620846793617	20.863507568661106
9	22.057725333219345	27.367916473447877	29.543958157700295	21.03040003563248
10	22.0647991663832	27.990604758113808	28.990155100472904	20.95444097503009
11	23.18976597019087	27.663848353656544	28.16503282123181	20.981352854920775
12	21.980620395634688	28.66070938600066	28.195893956445815	21.162776261918832
13	22.514367991804722	27.69754594915792	28.49827958180493	21.289806477232425
14	22.63149375466489	27.976007841515067	28.20556999361528	21.18692841020476
15	22.309838395725464	28.106634651766022	28.07930017997592	21.504226772532597
16	22.488584721609854	27.762648661577522	28.27844345889059	21.47032315792204
17	22.546222014883448	27.841756171399652	28.232386016857365	21.379635796859535
18	22.323908021355454	27.70602542180859	28.24050377886173	21.729562777974223
19	22.20646687316928	27.775938787530542	28.4215862754079	21.596008063892278
20	22.23358018953398	27.88838725863546	28.343536048506103	21.534496503324448
21	22.154804457103204	27.76842714891885	28.295218090194567	21.781550303783373
22	22.12936021689586	27.83412974706868	28.3948131660475	21.64169686998796
23	22.37398744517244	27.865975376604457	28.1890419340571	21.570995244166006
24	22.125879691274164	27.851442122008464	28.231038416074057	21.791639770643318
25	22.22194266008921	27.91628783501553	28.11642237229517	21.74534713260009
26	22.251866366317998	27.9123601923676	28.109889172492863	21.725884268821538
27	22.24734948006005	27.99848528868113	27.943779384635036	21.810385846623788
28	22.229725122293484	27.97807510237481	27.969964074607102	21.822235700724608
29	22.35562237559641	27.93613347693044	27.927604024677827	21.780640122795322
30	22.174873354797437	27.916278152780087	27.96089777187398	21.94795072054849
31	22.127852510898848	27.91212069430962	28.128266781925433	21.831760012866095
32	22.414056707382912	27.916256369155835	27.876084492451874	21.793602431009383
33	22.267969959378355	27.861868451959786	27.891014750936527	21.979146837725335
34	22.215234731988964	27.841597854882483	28.02495199774145	21.918215415387102
35	22.37033345418261	27.925885017360258	27.839865242872918	21.863916285584214
36	22.294425711051698	27.84980165593852	27.817417852366493	22.038354780643285
37	22.370575437852676	27.78424718443079	27.864820340724744	21.98035703699179
38	22.458811183987905	27.86632505556103	27.924348759558736	21.750515000892324
39	22.286851543417498	27.810095436117514	27.81606693838201	22.086986082082973
40	22.21272671059211	27.990145315540595	27.853996965157656	21.943131008709642
41	22.351742349481892	27.863162873002512	27.830855214973504	21.954239562542092
42	22.18564544397779	27.774334834072906	27.879422593148423	22.160597128800884
43	22.23475631278751	27.887703192735415	27.918930366643757	21.958610127833317
44	22.34241598448828	27.808110796337893	27.871287110917482	21.978186108256345
45	22.391017065982425	27.635522733179645	27.903334252515155	22.07012594832277
46	22.239316500828366	27.71108949328803	27.978146600637317	22.071447405246293
47	22.362740540842598	27.812870423060083	27.81430786892003	22.01008116717729
48	22.33029138144052	27.723179795910685	27.734473859646307	22.212054963002483
49	22.157129581649468	27.926347911768996	27.873577055511994	22.04294545106954
50	22.251273254134247	27.802960275335575	27.94283991328399	22.002926557246184
51	22.36631035033986	27.408983256842834	27.956611248140483	22.268095144676824
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.70579818106789
2	41.067127775677235
3	42.6022777733682
4	43.49863840351448
5	43.87919718903422
6	43.45856891650388
7	43.92850347340645
8	42.96588974560578
9	43.08812536885183
10	43.01924014141329
11	44.17111882511165
12	43.143396657553524
13	43.80417446903715
14	43.81842216486965
15	43.81406516825806
16	43.958907879531885
17	43.925857811742986
18	44.05347079932967
19	43.80247493706156
20	43.76807669285843
21	43.93635476088658
22	43.77105708688382
23	43.944982689338445
24	43.91751946191748
25	43.9672897926893
26	43.977750635139536
27	44.05773532668383
28	44.05196082301809
29	44.13626249839173
30	44.12282407534593
31	43.95961252376494
32	44.207659138392295
33	44.247116797103686
34	44.13345014737607
35	44.23424973976683
36	44.33278049169498
37	44.35093247484447
38	44.20932618488023
39	44.37383762550047
40	44.15585771930175
41	44.30598191202398
42	44.34624257277867
43	44.19336644062082
44	44.32060209274463
45	44.46114301430519
46	44.310763906074655
47	44.37282170801989
48	44.542346344443004
49	44.20007503271901
50	44.25419981138043
51	44.63440549501668
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3365.0
1	4083.5
2	4802.0
3	5455.0
4	6108.0
5	6904.0
6	7700.0
7	7923.0
8	8146.0
9	7350.5
10	6555.0
11	6634.0
12	6713.0
13	7492.5
14	8272.0
15	10211.0
16	12150.0
17	16615.0
18	21080.0
19	25210.0
20	29340.0
21	33777.5
22	38215.0
23	44408.5
24	50602.0
25	61928.0
26	88690.5
27	104127.0
28	111599.5
29	119072.0
30	135331.0
31	151590.0
32	166668.0
33	181746.0
34	218243.0
35	254740.0
36	291776.5
37	328813.0
38	372137.5
39	415462.0
40	442820.5
41	470179.0
42	506186.5
43	542194.0
44	556405.5
45	570617.0
46	548543.0
47	526469.0
48	461323.5
49	396178.0
50	356387.0
51	316596.0
52	277372.0
53	238148.0
54	207159.0
55	176170.0
56	157417.0
57	138664.0
58	119258.0
59	99852.0
60	95147.0
61	90442.0
62	72805.0
63	55168.0
64	47429.0
65	39690.0
66	33025.0
67	26360.0
68	22720.0
69	19080.0
70	16969.5
71	14859.0
72	12820.5
73	10782.0
74	9121.0
75	6382.0
76	5304.0
77	4943.0
78	4582.0
79	4496.5
80	4411.0
81	3927.5
82	3444.0
83	3021.5
84	2599.0
85	2373.5
86	2148.0
87	2430.0
88	2712.0
89	1882.5
90	1053.0
91	710.5
92	368.0
93	303.0
94	238.0
95	173.5
96	109.0
97	96.0
98	83.0
99	101.0
100	119.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0025009244488587747
2	0.12097328834051158
3	0.07227671657201859
4	0.13935508303962357
5	0.17533266761106336
6	0.3623124976553833
7	0.48285705609037627
8	0.28165768417968784
9	0.5354836519927902
10	0.4827677373600599
11	0.5665665701428921
12	0.7149964361826603
13	0.6726414942666307
14	0.6748923262706036
15	0.6642812611090171
16	0.8167661975051492
17	0.6319300169884224
18	0.4268899396741296
19	0.7024739501923032
20	0.5294992970615924
21	0.3709406870039461
22	0.6265887569155028
23	0.44257430871768677
24	0.7884164325027287
25	0.601525921188725
26	0.7373618462538831
27	0.6987404272650783
28	0.45141686301900885
29	0.7273224209663215
30	0.5114569135376827
31	0.4769262923973683
32	0.5689067208771814
33	0.526444596484772
34	0.7213202022890605
35	0.5674954849381825
36	0.5418431455913167
37	0.5637798257570209
38	0.8043508939911718
39	0.6822164621565472
40	0.7936683738453321
41	0.5838944038242707
42	0.726518552393474
43	0.8051904900561457
44	0.6097968356160224
45	0.8197851705898431
46	0.7469725416359261
47	0.5806253382946911
48	0.8278238563183178
49	0.5780350951155159
50	0.821982411355626
51	0.6551171593785561
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5597930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.6870055615943
#Duplication Level	Relative count
1	100.0
2	3.809737306283294
3	1.10198037908597
4	0.6147021172417455
5	0.4648611020983641
6	0.35513500439494317
7	0.3138402364420428
8	0.24422905617858218
9	0.21532271861155192
10++	10.865243373664557
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1861860	4.233853	4.626218	36
TTTGA	768085	2.1028535	11.051119	8
TGTTT	776910	2.0993612	10.875023	6
CTTTG	535645	1.8923075	13.725135	1
GTTTG	546130	1.8722193	13.0816145	7
TGTGT	459335	1.5746725	12.569287	4
TGAGG	347425	1.5309091	5.3101997	10
TTGAG	434880	1.5104748	8.140751	9
TTTGT	546170	1.475857	10.395703	2
GTGTT	418215	1.4337066	12.633729	5
TTGTG	342255	1.173304	12.405915	3
>>END_MODULE