FastQCFastQC Report
Tue 12 Aug 2014
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane3_Undetermined_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5597930
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTTT 624930 1.9767568 15.065401 6
GTTTG 465500 1.796809 17.60187 7
TGTGT 438490 1.6925516 17.50755 4
TTTGA 545070 1.6203951 13.810386 8
TTTGT 500555 1.5833381 14.687544 2
CTTTG 398570 1.5716829 17.88276 1
GTGTT 361580 1.3956822 17.348925 5
TTGAG 379125 1.3753442 10.152355 9
TGAGG 304900 1.3497293 6.0916486 10
TTGTG 324020 1.2507024 17.270288 3
Produced by FastQC (version 0.10.1)