##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane3_Undetermined_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5597930	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.605982389919134	34.0	31.0	34.0	31.0	34.0
2	32.893268047296054	34.0	31.0	34.0	31.0	34.0
3	32.991972747069006	34.0	31.0	34.0	31.0	34.0
4	36.4358021625851	37.0	37.0	37.0	35.0	37.0
5	36.365936873094164	37.0	37.0	37.0	35.0	37.0
6	36.362380379890425	37.0	37.0	37.0	35.0	37.0
7	36.361275685833874	37.0	37.0	37.0	35.0	37.0
8	36.34354127329209	37.0	37.0	37.0	35.0	37.0
9	38.09084804561686	39.0	38.0	39.0	37.0	39.0
10	38.07418795876333	39.0	38.0	39.0	37.0	39.0
11	38.07811530333534	39.0	38.0	39.0	37.0	39.0
12	38.04745718506662	39.0	38.0	39.0	37.0	39.0
13	38.04017860173314	39.0	38.0	39.0	37.0	39.0
14	39.57308594426868	41.0	40.0	41.0	37.0	41.0
15	39.57857172204726	41.0	40.0	41.0	37.0	41.0
16	39.56137125687531	41.0	40.0	41.0	37.0	41.0
17	39.462786601475905	41.0	40.0	41.0	37.0	41.0
18	39.45632367678767	41.0	39.0	41.0	37.0	41.0
19	39.49386880507616	41.0	39.0	41.0	37.0	41.0
20	39.47443465709646	41.0	39.0	41.0	37.0	41.0
21	39.43801297979789	41.0	39.0	41.0	37.0	41.0
22	39.42097221651575	41.0	39.0	41.0	37.0	41.0
23	39.396299167728074	40.0	39.0	41.0	37.0	41.0
24	39.351901685087164	41.0	39.0	41.0	37.0	41.0
25	39.299987495377756	40.0	39.0	41.0	36.0	41.0
26	39.216261903953786	40.0	39.0	41.0	36.0	41.0
27	39.11934357878716	40.0	39.0	41.0	36.0	41.0
28	39.08115571291531	40.0	39.0	41.0	36.0	41.0
29	39.00130762621183	40.0	39.0	41.0	36.0	41.0
30	38.91061410199842	40.0	39.0	41.0	36.0	41.0
31	38.835976155471755	40.0	38.0	41.0	35.0	41.0
32	38.66059186163457	40.0	38.0	41.0	35.0	41.0
33	38.58379061545964	40.0	38.0	41.0	35.0	41.0
34	38.69790922716076	40.0	38.0	41.0	35.0	41.0
35	38.680774143299395	40.0	38.0	41.0	35.0	41.0
36	38.661507735895235	40.0	38.0	41.0	35.0	41.0
37	38.65690532035949	40.0	38.0	41.0	35.0	41.0
38	38.62973599169693	40.0	38.0	41.0	35.0	41.0
39	38.55325790068829	40.0	38.0	41.0	35.0	41.0
40	38.520435053671626	40.0	38.0	41.0	35.0	41.0
41	38.43554224507988	40.0	38.0	41.0	35.0	41.0
42	37.772318339100345	40.0	38.0	41.0	33.0	41.0
43	37.998016588274595	40.0	38.0	41.0	33.0	41.0
44	38.11839054793468	40.0	38.0	41.0	34.0	41.0
45	38.10589789440025	40.0	38.0	41.0	34.0	41.0
46	38.056768305427184	40.0	38.0	41.0	34.0	41.0
47	37.90404988987	40.0	38.0	41.0	34.0	41.0
48	37.83791597965676	40.0	38.0	41.0	33.0	41.0
49	37.68171449089217	40.0	37.0	41.0	33.0	41.0
50	37.78722241971586	40.0	37.0	41.0	33.0	41.0
51	37.523800583429946	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	6.0
11	12.0
12	16.0
13	23.0
14	48.0
15	63.0
16	166.0
17	323.0
18	709.0
19	1746.0
20	2611.0
21	4312.0
22	6837.0
23	11383.0
24	15629.0
25	16927.0
26	19172.0
27	21919.0
28	25158.0
29	29171.0
30	34200.0
31	43051.0
32	55531.0
33	73709.0
34	104454.0
35	160492.0
36	268678.0
37	524972.0
38	1323556.0
39	2852098.0
40	952.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.408300763852818	30.6619122640466	27.386667052622382	23.543119919478205
2	20.905194974553005	30.21059645474829	29.282768033069484	19.601440537629223
3	19.655841051538626	28.779538615560845	30.01164072811361	21.552979604786916
4	20.37518412538454	28.66920490543449	29.436396241938567	21.519214727242407
5	23.745634547055786	28.625688424113914	27.506096003344094	20.122581025486205
6	22.393688381240924	28.073787989488974	28.17620084566974	21.35632278360037
7	23.691400214007675	28.807469904053818	26.663945422683028	20.837184459255475
8	21.799397277207824	28.463378427382978	28.44681873478232	21.290405560626873
9	21.584067682161084	28.204246926989086	28.834033294449913	21.377652096399917
10	21.702317027833026	28.758499910407377	28.16443739137006	21.37474567038954
11	23.247839550118346	28.537268285240664	26.8783493382207	21.336542826420292
12	21.80487165250746	29.87072356644751	26.873531112021	21.450873669024034
13	22.601174077685197	28.96944332597465	26.99205027004644	21.4373323262937
14	22.401226882079627	28.930694024398306	27.21245174555595	21.45562734796612
15	22.32814489864858	28.545477508615285	27.301475071272275	21.82490252146386
16	22.23833059308385	28.81301754891265	27.23404779933726	21.714604058666236
17	22.471043369602967	28.818318850542614	27.09504577564385	21.61559200421057
18	22.181746527707062	28.53280996598898	27.23280592854292	22.052637577761033
19	22.13543220440413	28.89028623080317	27.24828642015888	21.72599514463382
20	22.215872652927064	28.889393043500007	27.13177906833419	21.76295523523874
21	22.227644861582764	28.64728569310441	27.089852856323677	22.035216588989144
22	22.193886300625948	28.902163660788293	27.095171063537887	21.808778975047876
23	22.38859721361289	28.780960104895918	27.137638377042943	21.692804304448252
24	22.209209475645462	28.603680288963957	27.13660227977127	22.05050795561931
25	22.214249418060735	28.963619236978815	26.944127398422385	21.878003946538062
26	22.429497765588906	28.773505734788873	26.919358324251558	21.877638175370663
27	22.427659083881323	28.619494556445485	26.899524370429816	22.053321989243372
28	22.360739591376642	28.933012795368985	26.740182154817838	21.96606545843654
29	22.557355584072983	28.9437581322015	26.75961989630092	21.739266387424603
30	22.375668250714682	28.744334507776394	26.830023888461202	22.04997335304773
31	22.273591328700938	28.92599987985136	26.792628451770334	22.00778033967737
32	22.516781713088747	28.945374918624605	26.76768872365724	21.770154644629404
33	22.45614714257448	28.659441017655844	26.77827387353286	22.106137966236812
34	22.33829049425784	28.92408424716246	26.72314569231738	22.01447956626232
35	22.554331045634775	28.790284246186808	26.800144471107934	21.855240237070483
36	22.646038273553525	28.63295021494888	26.612206368875796	22.108805142621794
37	22.53225994808604	28.863868070048742	26.634096853996823	21.969775127868395
38	22.61933543291632	28.791812127795907	26.771079909870508	21.817772529417265
39	22.59974651403057	28.54649296644499	26.760860562655743	22.0928999568687
40	22.49678719603653	28.98500452835322	26.467061152456946	22.05114712315331
41	22.489444856379215	28.961897478152267	26.74207613331624	21.80658153215228
42	22.6363125815563	28.776222796911615	26.650229129666346	21.937235491865735
43	22.4994484611618	29.047622464042362	26.480195720058614	21.972733354737226
44	22.59630780384608	28.969049971236434	26.5956078784236	21.83903434649389
45	22.59639443711927	28.73203637419778	26.624410589175213	22.04715859950774
46	22.527831470732934	28.882323263551907	26.527784872118165	22.06206039359699
47	22.59979805698558	28.836571481126803	26.59218738127405	21.971443080613557
48	22.462897878073242	28.765377985850236	26.59393691675697	22.177787219319544
49	22.46479871145522	29.142684550701336	26.42767225517371	21.96484448266973
50	22.444320987543517	28.89228943842168	26.672811589312147	21.990577984722655
51	22.518127211943444	28.641030767888765	26.684505204921226	22.156336815246565
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.95142068333102
2	40.50663551218223
3	41.20882065632554
4	41.89439885262695
5	43.86821557254199
6	43.75001116484129
7	44.528584673263154
8	43.089802837834704
9	42.961719778561005
10	43.07706269822256
11	44.58438237653864
12	43.2557453215315
13	44.0385064039789
14	43.85685423004575
15	44.15304742011244
16	43.952934651750084
17	44.086635373813536
18	44.234384105468095
19	43.861427349037946
20	43.9788278881658
21	44.262861450571904
22	44.00266527567382
23	44.08140151806114
24	44.259717431264775
25	44.0922533645988
26	44.30713594095957
27	44.480981073124696
28	44.32680504981318
29	44.296621971497586
30	44.425641603762415
31	44.281371668378306
32	44.286936357718155
33	44.56228510881129
34	44.35277006052016
35	44.40957128270526
36	44.75484341617532
37	44.50203507595444
38	44.437107962333585
39	44.69264647089927
40	44.547934319189835
41	44.296026388531494
42	44.57354807342204
43	44.47218181589903
44	44.43534215033997
45	44.64355303662701
46	44.58989186432993
47	44.571241137599145
48	44.64068509739279
49	44.42964319412495
50	44.434898972266176
51	44.674464027190005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1753.0
1	1093.5
2	434.0
3	584.0
4	734.0
5	993.5
6	1253.0
7	2115.0
8	2977.0
9	2536.0
10	2095.0
11	2380.5
12	2666.0
13	3650.0
14	4634.0
15	6893.5
16	9153.0
17	14142.0
18	19131.0
19	23767.5
20	28404.0
21	32563.0
22	36722.0
23	43334.5
24	49947.0
25	62231.0
26	93304.0
27	112093.0
28	114567.5
29	117042.0
30	128762.5
31	140483.0
32	160786.0
33	181089.0
34	211471.5
35	241854.0
36	288017.0
37	334180.0
38	362871.0
39	391562.0
40	428571.5
41	465581.0
42	507818.0
43	550055.0
44	562118.0
45	574181.0
46	542562.0
47	510943.0
48	476921.0
49	442899.0
50	401122.0
51	359345.0
52	313137.0
53	266929.0
54	229972.0
55	193015.0
56	166346.0
57	139677.0
58	116205.0
59	92733.0
60	83274.5
61	73816.0
62	60169.5
63	46523.0
64	39765.5
65	33008.0
66	27527.5
67	22047.0
68	20116.5
69	18186.0
70	16207.5
71	14229.0
72	12156.0
73	10083.0
74	8479.5
75	5819.0
76	4762.0
77	4547.0
78	4332.0
79	4325.5
80	4319.0
81	3796.0
82	3273.0
83	2865.5
84	2458.0
85	2207.5
86	1957.0
87	2361.0
88	2765.0
89	1756.5
90	748.0
91	438.5
92	129.0
93	89.5
94	50.0
95	38.0
96	26.0
97	32.5
98	39.0
99	132.0
100	225.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025598748108675885
2	3.572749212655392E-4
3	0.006109401153640721
4	0.05782494600682752
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.006627449789475753
11	0.0065917222973491995
12	0.0028224718779977596
13	7.145498425310785E-5
14	0.0
15	5.537761279615857E-4
16	0.01993594060661709
17	0.21418631529869078
18	0.005930763693007952
19	0.0
20	0.0
21	0.0
22	1.786374606327696E-4
23	0.0
24	0.0
25	0.02011457806724986
26	0.060575962900572176
27	0.057878537245017354
28	0.034316256187555044
29	0.024151784677550452
30	0.011915118624205735
31	0.08678207837539947
32	0.391573313707031
33	0.32801410521389157
34	0.40663245163837347
35	0.48707290016130966
36	0.458651680174636
37	0.2652051740554098
38	0.24944935002759952
39	0.26756318853576233
40	0.19489346955035167
41	0.3798725600355846
42	0.5030073616497527
43	0.40407793595132485
44	0.2586313155041238
45	0.29869969792405404
46	0.3280676964520814
47	0.5540083566604084
48	0.3527196660194036
49	0.28258659897497823
50	0.481160000214365
51	0.4728890857870678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5597930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	61.26433658671385
#Duplication Level	Relative count
1	100.0
2	4.529186425030125
3	1.2385571348924205
4	0.6942154705737769
5	0.4640093041658923
6	0.4016618007637569
7	0.3135360026857386
8	0.2787652796345477
9	0.24759152793348002
10++	11.731522058426805
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTTT	624930	1.9767568	15.065401	6
GTTTG	465500	1.796809	17.60187	7
TGTGT	438490	1.6925516	17.50755	4
TTTGA	545070	1.6203951	13.810386	8
TTTGT	500555	1.5833381	14.687544	2
CTTTG	398570	1.5716829	17.88276	1
GTGTT	361580	1.3956822	17.348925	5
TTGAG	379125	1.3753442	10.152355	9
TGAGG	304900	1.3497293	6.0916486	10
TTGTG	324020	1.2507024	17.270288	3
>>END_MODULE