FastQCFastQC Report
Tue 12 Aug 2014
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane2_Undetermined_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5341076
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1600080 4.278818 4.6130123 36
TTTTT 1870295 3.889502 4.7783623 18
GGGGG 450000 3.0963886 4.8607717 16
TTTGA 839340 2.3318105 14.8406515 8
TGTTT 861775 2.2767198 14.121619 6
GTTTG 643205 2.1587229 17.226341 7
TGTGT 617545 2.072603 16.800783 4
CTTTG 580505 2.0334427 18.159447 1
GAGGG 348375 1.9842564 6.147749 11
GTGTT 531275 1.7830638 16.885277 5
TTGAG 482305 1.7021935 10.839013 9
TGAGG 370250 1.6600229 6.9178934 10
TTTGT 602045 1.5905402 13.659103 2
TTGTG 452180 1.5176055 16.715729 3
AACTT 428500 1.3065448 8.124634 2
ACTTT 446055 1.2933685 7.5611825 3
TAACT 278845 0.85022986 7.713775 1