##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane2_Undetermined_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5341076	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99533015444828	33.0	31.0	34.0	27.0	34.0
2	31.050870274079603	34.0	31.0	34.0	27.0	34.0
3	31.088521301700258	34.0	31.0	34.0	27.0	34.0
4	34.38967503926175	37.0	35.0	37.0	30.0	37.0
5	34.39698704905154	37.0	35.0	37.0	30.0	37.0
6	34.444539077893666	37.0	35.0	37.0	30.0	37.0
7	34.45034221568837	37.0	35.0	37.0	30.0	37.0
8	34.47757605396366	37.0	35.0	37.0	31.0	37.0
9	36.03848700149558	39.0	37.0	39.0	30.0	39.0
10	36.040742726746444	39.0	37.0	39.0	30.0	39.0
11	36.05795910786516	39.0	37.0	39.0	30.0	39.0
12	36.02014519171792	39.0	37.0	39.0	30.0	39.0
13	35.98419194933755	39.0	37.0	39.0	30.0	39.0
14	37.36752819094879	40.0	38.0	41.0	31.0	41.0
15	37.3294278156686	40.0	38.0	41.0	30.0	41.0
16	37.30213331545928	40.0	38.0	41.0	30.0	41.0
17	37.268125935672884	40.0	38.0	41.0	30.0	41.0
18	37.27823513464328	40.0	38.0	41.0	30.0	41.0
19	37.266196174703374	40.0	38.0	41.0	30.0	41.0
20	37.27170555146566	40.0	38.0	41.0	30.0	41.0
21	37.26032881763899	40.0	38.0	41.0	30.0	41.0
22	37.22475958027933	40.0	38.0	41.0	30.0	41.0
23	37.18148964740438	40.0	38.0	41.0	30.0	41.0
24	37.12735055632985	40.0	38.0	41.0	30.0	41.0
25	37.089825907738444	40.0	38.0	41.0	30.0	41.0
26	37.03051651015638	40.0	38.0	41.0	29.0	41.0
27	36.963239616886185	40.0	38.0	41.0	29.0	41.0
28	36.90512117783008	40.0	38.0	41.0	29.0	41.0
29	36.851355045312964	40.0	38.0	41.0	29.0	41.0
30	36.80591719720895	40.0	38.0	41.0	29.0	41.0
31	36.77066343935192	40.0	38.0	41.0	28.0	41.0
32	36.705026103354456	40.0	38.0	41.0	28.0	41.0
33	36.60107457748214	40.0	37.0	41.0	28.0	41.0
34	36.522735868203334	40.0	37.0	41.0	27.0	41.0
35	36.46607050714126	40.0	37.0	41.0	27.0	41.0
36	36.40367858461479	40.0	37.0	41.0	27.0	41.0
37	36.31313915023864	40.0	37.0	41.0	27.0	41.0
38	36.271900456012986	40.0	37.0	41.0	27.0	41.0
39	36.18310804789147	40.0	37.0	41.0	26.0	41.0
40	36.11710018730308	40.0	36.0	41.0	26.0	41.0
41	36.281801082778074	40.0	37.0	41.0	27.0	41.0
42	36.30294401352836	40.0	37.0	41.0	27.0	41.0
43	36.30168321888698	40.0	37.0	41.0	27.0	41.0
44	36.26069728271981	40.0	37.0	41.0	27.0	41.0
45	36.20173088718453	40.0	36.0	41.0	27.0	41.0
46	36.11136950681848	40.0	36.0	41.0	26.0	41.0
47	36.063221343414696	40.0	36.0	41.0	26.0	41.0
48	35.97096277978445	40.0	36.0	41.0	26.0	41.0
49	35.887756324755536	40.0	36.0	41.0	26.0	41.0
50	35.75946064051513	39.0	35.0	41.0	25.0	41.0
51	34.37475576082422	38.0	34.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2162.0
3	2529.0
4	4378.0
5	2950.0
6	3481.0
7	9870.0
8	33909.0
9	51801.0
10	53748.0
11	37636.0
12	26218.0
13	21835.0
14	19981.0
15	18662.0
16	17459.0
17	16395.0
18	16190.0
19	16188.0
20	16341.0
21	16672.0
22	17579.0
23	18302.0
24	19509.0
25	20618.0
26	22624.0
27	25092.0
28	29323.0
29	33417.0
30	38724.0
31	47865.0
32	60991.0
33	79152.0
34	112067.0
35	166559.0
36	269671.0
37	489395.0
38	1190870.0
39	2310693.0
40	220.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.353191310789125	27.466344988364206	30.796344887199652	22.38411881364702
2	22.018158849992012	29.441686814410957	29.720835905718307	18.819318429878724
3	22.1331629053718	28.144085617928532	29.125952400280237	20.59679907641943
4	21.671694794809245	26.15486009678756	30.244012652507905	21.929432455895284
5	24.72374591978389	26.528852286796983	28.828387048362288	19.91901474505684
6	21.89946944929546	26.127253752416053	30.935077560280945	21.03819923800755
7	23.818603285327182	26.46188948370855	29.551478186941605	20.168029044022663
8	22.412238167576877	26.673338237575837	30.396322456008622	20.518101138838656
9	22.502530040651607	26.54210369005513	30.297159309619676	20.658206959673585
10	22.33036181579621	27.691881843634114	29.34649461898743	20.63126172158225
11	23.99530063688737	26.613799144890326	28.64115596363726	20.749744254585046
12	22.04557726925715	28.283294309358396	28.691245156641653	20.979883264742796
13	23.036107789385017	26.7975677444101	29.05667366109369	21.109650805111198
14	23.396182619693786	27.060280088692995	28.37730112356	21.166236168053214
15	22.740708164686417	27.692315795057272	28.150262944352257	21.416713095904058
16	23.11842656656422	26.97948869547742	28.453081870406372	21.449002867551993
17	23.012409105235236	27.187568304393412	28.463849109851036	21.336173480520316
18	22.83250166374638	26.952390635809536	28.520324769307305	21.69478293113678
19	22.626009580752257	27.090919318386835	28.73832483389456	21.544746266966346
20	22.574585384070772	27.171657882960474	28.73611047678976	21.517646256178995
21	22.52640988334168	27.132561593026967	28.630740678100047	21.7102878455313
22	22.490502935388026	27.194506157336345	28.730435520928292	21.584555386347333
23	22.65428880546348	27.20503634170716	28.57430159255152	21.56637326027784
24	22.42440929349826	27.274796929243973	28.557625661379387	21.74316811587838
25	22.406129868218123	27.401490295952513	28.426383262841625	21.76599657298774
26	22.344365634834894	27.484973460472546	28.471623843299355	21.69903706139321
27	22.39803950757627	27.50950323064732	28.297579683536405	21.79487757824001
28	22.403544055401877	27.47891927871779	28.297506374551677	21.820030291328656
29	22.578116745235572	27.363901319408647	28.263956180396764	21.794025754959016
30	22.419565630732762	27.349620740422314	28.28809373682474	21.94271989202018
31	22.395616974444177	27.344391010870652	28.36267820776798	21.89731380691719
32	22.643406879942262	27.376418934375145	28.151080023667124	21.829094162015465
33	22.463100559074174	27.315505616033704	28.167176548482775	22.05421727640935
34	22.48734400853153	27.324053213679196	28.209737427453597	21.97886535033568
35	22.562357871620765	27.448782079062628	28.065700326865894	21.923159722450713
36	22.43878847799049	27.344494781567736	28.15143122210861	22.065285518333173
37	22.493245734841473	27.26843594548424	28.158079907284073	22.08023841239022
38	22.545592579321227	27.279196113207206	28.316085715404142	21.859125592067425
39	22.390371302824523	27.246567429292902	28.17558655233859	22.18747471554399
40	22.38342821087447	27.381487127597577	28.1295804343322	22.105504227195752
41	22.580657900378437	27.28811857281157	28.132157775135003	21.99906575167499
42	22.41914870556641	27.200473125336817	28.186633870535566	22.193744298561207
43	22.489565592582704	27.297399569357832	28.146678762130513	22.066356075928955
44	22.614062238502576	27.261378017386463	28.086887191068705	22.037672553042256
45	22.67276376485589	27.08503709479958	28.10763268828438	22.13456645206015
46	22.47028249068237	27.19649253111885	28.18326460907356	22.149960369125225
47	22.632835873697555	27.22827975889952	28.035636292881644	22.103248074521282
48	22.495888140867457	27.173525537572175	27.952077688820825	22.378508632739546
49	22.303426831589533	27.348409215558085	28.11515610670282	22.23300784614956
50	22.439260923113874	27.25940368401506	28.177819222858307	22.123516170012756
51	22.50860446143868	26.96678515927199	28.16027989264103	22.364330486648303
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.73731012443615
2	40.83747727987073
3	42.729961981791234
4	43.60112725070453
5	44.64276066484073
6	42.93766868730301
7	43.986632329349845
8	42.93033930641553
9	43.16073700032519
10	42.96162353737846
11	44.74504489147241
12	43.02546053399995
13	44.145758594496215
14	44.562418787747006
15	44.157421260590475
16	44.567429434116214
17	44.348582585755544
18	44.52728459488316
19	44.1707558477186
20	44.09223164024977
21	44.23669772887299
22	44.07505832173536
23	44.220662065741315
24	44.16757740937664
25	44.17212644120586
26	44.0434026962281
27	44.19291708581628
28	44.22357434673054
29	44.37214250019459
30	44.36228552275294
31	44.29293078136137
32	44.47250104195773
33	44.517317835483524
34	44.46620935886721
35	44.485517594071474
36	44.50407399632366
37	44.57348414723169
38	44.40471817138865
39	44.57784601836851
40	44.48893243807022
41	44.579723652053424
42	44.61289300412762
43	44.55592166851166
44	44.651734791544825
45	44.80733021691604
46	44.62024285980759
47	44.736083948218834
48	44.874396773607
49	44.5364346777391
50	44.562777093126634
51	44.87293494808698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4055.0
1	3863.5
2	3672.0
3	4211.0
4	4750.0
5	7908.5
6	11067.0
7	12220.0
8	13373.0
9	9814.5
10	6256.0
11	6323.0
12	6390.0
13	6998.5
14	7607.0
15	9435.0
16	11263.0
17	15137.0
18	19011.0
19	22995.0
20	26979.0
21	31277.5
22	35576.0
23	41511.0
24	47446.0
25	58129.0
26	82911.0
27	97010.0
28	104351.5
29	111693.0
30	127507.5
31	143322.0
32	157621.0
33	171920.0
34	203924.5
35	235929.0
36	269486.5
37	303044.0
38	342778.5
39	382513.0
40	407557.5
41	432602.0
42	466995.5
43	501389.0
44	515421.5
45	529454.0
46	510202.0
47	490950.0
48	435638.0
49	380326.0
50	344848.0
51	309370.0
52	273748.5
53	238127.0
54	209259.0
55	180391.0
56	162112.0
57	143833.0
58	124558.0
59	105283.0
60	99256.0
61	93229.0
62	76150.5
63	59072.0
64	50578.5
65	42085.0
66	35339.0
67	28593.0
68	24962.0
69	21331.0
70	19063.5
71	16796.0
72	14568.0
73	12340.0
74	10520.5
75	7507.5
76	6314.0
77	5953.0
78	5592.0
79	5540.5
80	5489.0
81	4945.0
82	4401.0
83	4023.0
84	3645.0
85	3277.5
86	2910.0
87	3221.5
88	3533.0
89	2582.5
90	1632.0
91	1231.0
92	830.0
93	672.5
94	515.0
95	419.5
96	324.0
97	256.0
98	188.0
99	165.5
100	143.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060624488398966794
2	0.16476080849626554
3	0.1320520434459274
4	0.1450269571150083
5	0.1961402533871452
6	0.19803125812102282
7	0.2825647865710954
8	0.20589484216289003
9	0.33774093459819704
10	0.29887236204839623
11	0.3811029837433506
12	0.4541968696944211
13	0.4223119086865643
14	0.4635770020872199
15	0.46323999134256844
16	0.5015281564988029
17	0.3786690172542012
18	0.3791932561903257
19	0.47980968628793147
20	0.382488472360251
21	0.2585434096051058
22	0.32931566598191075
23	0.20031544205699375
24	0.24352770864896886
25	0.28372560135822816
26	0.3524757932671244
27	0.3890601818809543
28	0.3141689052917427
29	0.4152346830488838
30	0.348244436139834
31	0.25831873577533815
32	0.2277630949269398
33	0.4339387793770394
34	0.4905753072976307
35	0.41903541533578625
36	0.46657265315078833
37	0.485220581021502
38	0.5147277439976514
39	0.49701595708430285
40	0.5061339700090394
41	0.43871309825960164
42	0.49855122825438175
43	0.49965587458407257
44	0.43335837198347305
45	0.5027825853816722
46	0.5298370590495248
47	0.5044489162857821
48	0.5316157268685187
49	0.45548874421558505
50	0.534667546389529
51	0.5811563063322821
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5341076.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	55.285981595335585
#Duplication Level	Relative count
1	100.0
2	4.045055549965374
3	1.1932736301917168
4	0.6380699272552931
5	0.47766459303805336
6	0.36934659982124574
7	0.28292896588871064
8	0.24445536174066423
9	0.2391282473201655
10++	10.89039758030625
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1600080	4.278818	4.6130123	36
TTTTT	1870295	3.889502	4.7783623	18
GGGGG	450000	3.0963886	4.8607717	16
TTTGA	839340	2.3318105	14.8406515	8
TGTTT	861775	2.2767198	14.121619	6
GTTTG	643205	2.1587229	17.226341	7
TGTGT	617545	2.072603	16.800783	4
CTTTG	580505	2.0334427	18.159447	1
GAGGG	348375	1.9842564	6.147749	11
GTGTT	531275	1.7830638	16.885277	5
TTGAG	482305	1.7021935	10.839013	9
TGAGG	370250	1.6600229	6.9178934	10
TTTGT	602045	1.5905402	13.659103	2
TTGTG	452180	1.5176055	16.715729	3
AACTT	428500	1.3065448	8.124634	2
ACTTT	446055	1.2933685	7.5611825	3
TAACT	278845	0.85022986	7.713775	1
>>END_MODULE