FastQCFastQC Report
Tue 12 Aug 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename lane2_Undetermined_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5341076
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5493 0.10284444557613484 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1525150 4.0598474 4.9719462 17
TGTTT 653950 2.1126661 19.144915 6
TGTGT 515820 2.0224314 22.509382 4
GTTTG 499500 1.9584439 22.543749 7
TTTGA 574155 1.7751324 17.983385 8
CTTTG 431815 1.7379793 23.110313 1
TTTGT 523405 1.6909246 18.805862 2
GAGGG 293140 1.6201102 5.7184796 11
GTGTT 409905 1.6071593 22.342625 5
TTGAG 389960 1.4632244 12.409723 9
TTGTG 364790 1.430272 22.240696 3
TGAGG 313765 1.4288435 7.3824105 10
TTGAT 338460 1.046427 5.0229936 9
Produced by FastQC (version 0.10.1)