##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane2_Undetermined_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5341076	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70678960568994	34.0	31.0	34.0	31.0	34.0
2	32.95939844330992	34.0	31.0	34.0	31.0	34.0
3	33.02856334566293	34.0	31.0	34.0	31.0	34.0
4	36.40851862059255	37.0	37.0	37.0	35.0	37.0
5	36.33421561498095	37.0	37.0	37.0	35.0	37.0
6	36.31690281134363	37.0	37.0	37.0	35.0	37.0
7	36.29831105193036	37.0	37.0	37.0	35.0	37.0
8	36.27261304650973	37.0	37.0	37.0	35.0	37.0
9	38.03016583175375	39.0	38.0	39.0	37.0	39.0
10	38.0185713515404	39.0	38.0	39.0	37.0	39.0
11	38.002226143196616	39.0	38.0	39.0	35.0	39.0
12	37.960871554720434	39.0	38.0	39.0	35.0	39.0
13	37.969859069595714	39.0	38.0	39.0	35.0	39.0
14	39.449572146136845	41.0	39.0	41.0	37.0	41.0
15	39.40396204809667	41.0	39.0	41.0	37.0	41.0
16	39.43176880463787	41.0	39.0	41.0	37.0	41.0
17	39.38960258195165	41.0	39.0	41.0	37.0	41.0
18	39.32346665728029	41.0	39.0	41.0	36.0	41.0
19	39.38525420720469	41.0	39.0	41.0	36.0	41.0
20	39.341579486979775	41.0	39.0	41.0	36.0	41.0
21	39.316697234789395	40.0	39.0	41.0	36.0	41.0
22	39.260288563577824	40.0	39.0	41.0	36.0	41.0
23	39.23515055767789	40.0	39.0	41.0	36.0	41.0
24	39.19000946625736	40.0	39.0	41.0	36.0	41.0
25	39.083847337128326	40.0	39.0	41.0	36.0	41.0
26	38.99128827225076	40.0	39.0	41.0	36.0	41.0
27	38.94306671539592	40.0	39.0	41.0	36.0	41.0
28	38.88989952586333	40.0	39.0	41.0	35.0	41.0
29	38.81834858743819	40.0	38.0	41.0	35.0	41.0
30	38.73343255179294	40.0	38.0	41.0	35.0	41.0
31	38.6433847786476	40.0	38.0	41.0	35.0	41.0
32	38.49201490486187	40.0	38.0	41.0	35.0	41.0
33	38.37192037709256	40.0	38.0	41.0	34.0	41.0
34	38.38610684438866	40.0	38.0	41.0	35.0	41.0
35	38.45421091180878	40.0	38.0	41.0	35.0	41.0
36	38.42939306611627	40.0	38.0	41.0	35.0	41.0
37	38.43248888426227	40.0	38.0	41.0	35.0	41.0
38	38.40665701068474	40.0	38.0	41.0	35.0	41.0
39	38.3615797640775	40.0	38.0	41.0	34.0	41.0
40	38.27484143644464	40.0	38.0	41.0	34.0	41.0
41	38.140486486243596	40.0	38.0	41.0	34.0	41.0
42	37.946181069132884	40.0	38.0	41.0	33.0	41.0
43	37.97305786324703	40.0	38.0	41.0	33.0	41.0
44	37.64198730742644	40.0	38.0	41.0	33.0	41.0
45	37.75580706958673	40.0	38.0	41.0	33.0	41.0
46	37.76163623210005	40.0	37.0	41.0	33.0	41.0
47	37.64728024839938	40.0	37.0	41.0	33.0	41.0
48	37.60479292936479	40.0	37.0	41.0	33.0	41.0
49	37.35394590902657	40.0	37.0	41.0	32.0	41.0
50	37.232652559147255	40.0	37.0	41.0	32.0	41.0
51	37.09183280672284	40.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	6.0
11	8.0
12	7.0
13	23.0
14	41.0
15	54.0
16	111.0
17	215.0
18	503.0
19	1176.0
20	2409.0
21	4511.0
22	7357.0
23	11716.0
24	16299.0
25	21455.0
26	26555.0
27	30984.0
28	34741.0
29	38022.0
30	43188.0
31	49436.0
32	59691.0
33	76807.0
34	104832.0
35	159822.0
36	264952.0
37	506788.0
38	1240253.0
39	2638172.0
40	940.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.107207534180645	29.6689076089786	27.524955437721616	23.698929419119136
2	21.447226102538842	29.556629125422507	29.279118616738742	19.717026155299912
3	20.04703570403492	27.9466823839287	30.509924174071422	21.496357737964956
4	20.928267560002165	27.841937920027338	29.513944378361884	21.715850141608612
5	24.420023231273998	27.96679919926247	27.45068596664792	20.162491602815614
6	22.51164372122771	27.431326571649606	28.757426406214776	21.299603300907908
7	24.226485449748328	28.3771846721522	26.690033993150443	20.706295884949025
8	22.013654177547746	28.133020387652223	28.73497774605716	21.118347688742865
9	21.789858863169055	27.836582190270164	29.146584815218258	21.226974131342516
10	21.822381857138897	28.42148286225472	28.49648647575882	21.25964880484756
11	24.056877734776528	27.93223277593977	26.78615896482448	21.224730524459222
12	21.912850183103195	29.804480808107293	26.6874286618532	21.595240346936308
13	22.858819408050746	28.682710039156344	27.039708872872854	21.41876167992006
14	22.570545710265122	28.446122092252573	27.22417730060385	21.759154896878456
15	22.555078818340146	28.41400834528402	27.293955784116392	21.73695705225944
16	22.478501154893543	28.346307785869858	27.188025206937148	21.98716585229945
17	22.89483498216859	28.418898481847194	27.03353953925065	21.652726996733563
18	22.428270974798767	28.149985517609256	27.461327710383642	21.960415797208334
19	22.237298252262278	28.439643996827606	27.30803680756462	22.015020943345498
20	22.29698659970388	28.499950197301064	27.484255981379036	21.718807221616018
21	22.602561731007007	28.219070464453228	27.144698933323546	22.03366887121621
22	22.244525260453138	28.81795728051801	27.138183392260284	21.79933406676857
23	22.425106851128874	28.680887521540605	27.171154276778687	21.722851350551835
24	22.248615922235135	28.287424584636245	27.188587885872117	22.2753716072565
25	22.27312701756462	28.59409459853916	27.173568084971333	21.959210298924884
26	22.454038356043284	28.442419955166315	26.933867037352726	22.169674651437674
27	22.445593006363946	28.267087958612823	26.919454317824254	22.36786471719898
28	22.414517867685372	28.83229453364863	26.762852770437284	21.990334828228715
29	22.85288582300645	28.524795378309538	26.809073677288996	21.813245121395013
30	22.449247833466426	28.42420900577711	27.053916892361872	22.072626268394586
31	22.346733130178265	28.54467901224397	26.783704257344397	22.32488360023336
32	22.63722310911777	28.802593546237	26.7331060456536	21.827077298991636
33	22.561530303831727	28.366155622554366	26.736726564202755	22.335587509411155
34	22.583545626026243	28.570584216606658	26.637161781948933	22.20870837541817
35	22.76257562256718	28.480476480795385	26.789888852412886	21.967059044224545
36	22.722143671445185	28.313454056612464	26.75895533573844	22.205446936203906
37	22.666777304901306	28.57012007182994	26.694764532553773	22.068338090714988
38	22.653712711472807	28.51704510015065	26.829308495324895	21.99993369305165
39	22.63399501514097	28.294929062702256	26.861009354338055	22.210066567818718
40	22.563810039228795	28.733903432023094	26.546111919190253	22.156174609557855
41	22.559029766117465	28.722453338697	26.802620943380507	21.915895951805023
42	22.584065207212085	28.309409828469466	26.810127589939846	22.2963973743786
43	22.59041444605148	28.613792107272364	26.626803458438147	22.168989988238007
44	22.845365698986665	28.60789916565508	26.559861011307838	21.98687412405041
45	22.782390244707457	28.32260772243984	26.67856623124112	22.21643580161158
46	22.676787884523662	28.60103617232807	26.608339416246483	22.113836526901782
47	22.670968643292476	28.614779550613452	26.707282814276894	22.006968991817182
48	22.60527613301382	28.429969772310713	26.677153441865276	22.287600652810184
49	22.613420279465966	28.70912385420884	26.407264713613802	22.270191152711387
50	22.57626530217436	28.366572087994157	26.57177681432837	22.485385795503113
51	22.69523519847394	28.262306285602705	26.636964105337075	22.405494410586275
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.806136953299784
2	41.164252257838754
3	41.543393441999875
4	42.64411770161078
5	44.58251483408961
6	43.81124702213561
7	44.93278133469735
8	43.132001866290615
9	43.016832994511574
10	43.08203066198646
11	45.28160825923575
12	43.5080905300395
13	44.2775810879708
14	44.32970060714358
15	44.29203587059959
16	44.46566700719299
17	44.54756197890216
18	44.388686772007105
19	44.252319195607775
20	44.015793821319896
21	44.63623060222322
22	44.04385932722171
23	44.14795820168071
24	44.523987529491635
25	44.2323373164895
26	44.62371300748096
27	44.81345772356293
28	44.40485269591409
29	44.666130944401466
30	44.521874101861016
31	44.67161673041162
32	44.4643004081094
33	44.89711781324288
34	44.79225400144441
35	44.72963466679173
36	44.927590607649094
37	44.73511539561629
38	44.65364640452446
39	44.84406158295969
40	44.71998464878665
41	44.47492571792249
42	44.880462581590685
43	44.759404434289486
44	44.83223982303707
45	44.99882604631903
46	44.79062441142545
47	44.67793763510966
48	44.89287678582401
49	44.883611432177354
50	45.061651097677476
51	45.10072960906022
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4552.0
1	2745.5
2	939.0
3	1095.5
4	1252.0
5	2586.0
6	3920.0
7	6671.0
8	9422.0
9	6105.0
10	2788.0
11	3016.0
12	3244.0
13	3963.5
14	4683.0
15	6691.0
16	8699.0
17	12919.5
18	17140.0
19	21553.0
20	25966.0
21	30096.0
22	34226.0
23	40211.0
24	46196.0
25	57376.0
26	85071.5
27	101587.0
28	105229.5
29	108872.0
30	120200.0
31	131528.0
32	149996.5
33	168465.0
34	196073.5
35	223682.0
36	263762.5
37	303843.0
38	330781.5
39	357720.0
40	391741.5
41	425763.0
42	463584.0
43	501405.0
44	514724.0
45	528043.0
46	503321.0
47	478599.0
48	451390.0
49	424181.0
50	386610.0
51	349039.0
52	309240.5
53	269442.0
54	237389.0
55	205336.0
56	177236.0
57	149136.0
58	125108.5
59	101081.0
60	90824.5
61	80568.0
62	66659.0
63	52750.0
64	45241.0
65	37732.0
66	31729.5
67	25727.0
68	23448.0
69	21169.0
70	18867.5
71	16566.0
72	14126.5
73	11687.0
74	9765.0
75	6495.5
76	5148.0
77	4881.5
78	4615.0
79	4717.5
80	4820.0
81	4199.5
82	3579.0
83	3123.5
84	2668.0
85	2484.0
86	2300.0
87	2751.5
88	3203.0
89	2077.5
90	952.0
91	556.5
92	161.0
93	112.0
94	63.0
95	41.0
96	19.0
97	21.5
98	24.0
99	100.5
100	177.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025425588402037343
2	3.1828792550414934E-4
3	5.055161169771784E-4
4	0.0650992421751722
5	0.0
6	0.0
7	0.0
8	0.0
9	3.3701074465145226E-4
10	0.0
11	0.0027335315955062237
12	0.02229887760443776
13	0.0033513846273672196
14	0.0
15	0.03319555834816805
16	0.006066193403726141
17	0.09335197626845226
18	0.0020033416487614105
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.00290203696783195
25	0.003164156435894191
26	0.03345767781623029
27	0.012694071381871369
28	0.008556328350317428
29	0.0
30	0.003201602074188796
31	0.0
32	0.11209351823490248
33	0.18045053094170538
34	0.21649195780026348
35	0.1933318305150498
36	0.19627131312117632
37	0.04409223909189834
38	0.042482076645230286
39	0.05450212653779875
40	0.08781002180085061
41	0.2132903557260747
42	0.27698538646519916
43	0.19370628689799582
44	0.20868454221583815
45	0.16228939636882156
46	0.09531787227891908
47	0.2952963035912614
48	0.05474552318671369
49	0.07161478323843361
50	0.122447237223361
51	0.27964402678411615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5341076.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	57.5668681651101
#Duplication Level	Relative count
1	100.0
2	4.564297952386087
3	1.2766135892551191
4	0.6961168421935469
5	0.45708876987407593
6	0.3917903741777794
7	0.31331248577213855
8	0.26478798988773466
9	0.2516084971783904
10++	11.59795358422295
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5493	0.10284444557613484	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1525150	4.0598474	4.9719462	17
TGTTT	653950	2.1126661	19.144915	6
TGTGT	515820	2.0224314	22.509382	4
GTTTG	499500	1.9584439	22.543749	7
TTTGA	574155	1.7751324	17.983385	8
CTTTG	431815	1.7379793	23.110313	1
TTTGT	523405	1.6909246	18.805862	2
GAGGG	293140	1.6201102	5.7184796	11
GTGTT	409905	1.6071593	22.342625	5
TTGAG	389960	1.4632244	12.409723	9
TTGTG	364790	1.430272	22.240696	3
TGAGG	313765	1.4288435	7.3824105	10
TTGAT	338460	1.046427	5.0229936	9
>>END_MODULE