FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_8_TGACCA_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_8_TGACCA_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 32168050
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 8450070 3.2454143 3.7414951 16
TGTGT 3846955 2.2889898 15.594852 4
TGTTT 4402405 2.1045454 12.984918 6
GTTTG 3174200 1.8886915 15.327982 7
TTTGA 3786440 1.8380966 12.852277 8
CTTTG 2906690 1.7757188 15.904836 1
TTTGT 3398935 1.6248422 12.5978155 2
GTGTT 2694150 1.6030554 15.182971 5
AACTT 3111255 1.5746733 5.008721 2
TGAGG 1908990 1.4356805 5.6913743 10
TTGTG 2403500 1.4301147 15.05629 3
TTGAG 2309915 1.3956987 9.195056 9
Produced by FastQC (version 0.10.1)