##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_8_TGACCA_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	32168050	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16330822042368	33.0	31.0	34.0	31.0	34.0
2	32.336706203826466	34.0	31.0	34.0	31.0	34.0
3	32.39117605823169	34.0	31.0	34.0	31.0	34.0
4	35.871204720211516	37.0	35.0	37.0	35.0	37.0
5	35.885708086128936	37.0	37.0	37.0	35.0	37.0
6	35.90469646124027	37.0	37.0	37.0	35.0	37.0
7	35.91676100975968	37.0	37.0	37.0	35.0	37.0
8	35.928732546735034	37.0	37.0	37.0	35.0	37.0
9	37.66072522269768	39.0	38.0	39.0	35.0	39.0
10	37.64221228827983	39.0	38.0	39.0	35.0	39.0
11	37.60482795817589	39.0	38.0	39.0	35.0	39.0
12	37.58323662764762	39.0	38.0	39.0	35.0	39.0
13	37.56562688754836	39.0	38.0	39.0	35.0	39.0
14	39.033479803718286	41.0	39.0	41.0	36.0	41.0
15	38.99663131585533	41.0	39.0	41.0	36.0	41.0
16	38.95098944449539	41.0	39.0	41.0	36.0	41.0
17	38.939036963695344	41.0	39.0	41.0	36.0	41.0
18	38.880229731053014	41.0	39.0	41.0	36.0	41.0
19	38.89172703971798	41.0	39.0	41.0	36.0	41.0
20	38.871814424561016	41.0	39.0	41.0	35.0	41.0
21	38.82940613434759	41.0	39.0	41.0	35.0	41.0
22	38.75456886569127	40.0	39.0	41.0	35.0	41.0
23	38.72299343603358	40.0	39.0	41.0	35.0	41.0
24	38.673267139288825	40.0	39.0	41.0	35.0	41.0
25	38.62812299781926	40.0	39.0	41.0	35.0	41.0
26	38.568703791494976	40.0	39.0	41.0	34.0	41.0
27	38.519127674820204	40.0	38.0	41.0	34.0	41.0
28	38.44228154954994	40.0	38.0	41.0	34.0	41.0
29	38.37424932502903	40.0	38.0	41.0	34.0	41.0
30	38.3285886772745	40.0	38.0	41.0	34.0	41.0
31	38.26892326392181	40.0	38.0	41.0	34.0	41.0
32	38.199341489459265	40.0	38.0	41.0	34.0	41.0
33	38.13771372526467	40.0	38.0	41.0	33.0	41.0
34	38.07801197150589	40.0	38.0	41.0	33.0	41.0
35	38.017038490054574	40.0	38.0	41.0	33.0	41.0
36	37.9404219404036	40.0	38.0	41.0	33.0	41.0
37	37.86066733917661	40.0	38.0	41.0	33.0	41.0
38	37.77872662470992	40.0	38.0	41.0	33.0	41.0
39	37.708286918230975	40.0	38.0	41.0	32.0	41.0
40	37.596879108307775	40.0	37.0	41.0	32.0	41.0
41	37.789502285653	40.0	38.0	41.0	33.0	41.0
42	37.82279283326157	40.0	38.0	41.0	33.0	41.0
43	37.80255436683293	40.0	38.0	41.0	33.0	41.0
44	37.75022530119171	40.0	38.0	41.0	33.0	41.0
45	37.68548606458893	40.0	37.0	41.0	33.0	41.0
46	37.62662306232426	40.0	37.0	41.0	32.0	41.0
47	37.559563200131805	40.0	37.0	41.0	32.0	41.0
48	37.48122512866027	40.0	37.0	41.0	32.0	41.0
49	37.40081024494801	40.0	37.0	41.0	32.0	41.0
50	37.32693082110976	40.0	36.0	41.0	32.0	41.0
51	35.784356776366614	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	19.0
6	343.0
7	1959.0
8	6588.0
9	10762.0
10	14751.0
11	19569.0
12	27574.0
13	37526.0
14	45444.0
15	51371.0
16	53980.0
17	55708.0
18	56900.0
19	59000.0
20	61858.0
21	66501.0
22	73337.0
23	81739.0
24	92024.0
25	106614.0
26	122854.0
27	144444.0
28	172401.0
29	207423.0
30	257167.0
31	322582.0
32	418734.0
33	566275.0
34	810809.0
35	1193266.0
36	1812086.0
37	2960922.0
38	6431529.0
39	1.582106E7
40	2930.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.25279521361878	28.734480469448847	27.426317542608814	23.586406774323564
2	23.12915144063753	29.354785260530246	28.910157749692626	18.605905549139596
3	21.749658434378212	27.743664909747405	29.401673399537742	21.105003256336644
4	21.756780407889195	26.88165431227569	29.625892772486985	21.73567250734813
5	23.97211238967597	27.205164806123765	28.31813000259922	20.50459280160105
6	22.291411074985966	26.8984031929178	29.501838394846523	21.308347337249707
7	23.593199306057222	27.49005392633898	28.07807751780629	20.83866924979751
8	21.753728993160813	27.1144619022906	29.745490928012465	21.386318176536133
9	21.95583172935708	26.91965117340428	29.86182632686495	21.26269077037369
10	22.014964592436154	27.669222588605336	29.14519810475314	21.17061471420537
11	23.399391101713174	27.107309073862883	28.140382996686647	21.352916827737296
12	21.79783102217163	28.611217111220345	28.177308358988952	21.413643507619074
13	22.84459856486002	27.305385211729266	28.360965463456537	21.489050759954175
14	22.88825914314426	27.418654340965766	28.084371394103876	21.6087151217861
15	22.575476879797442	27.646261340795814	27.992328101620195	21.78593367778655
16	22.648892758715856	27.403099575917068	28.133137941623488	21.81486972374359
17	22.63199696433422	27.429469920552528	28.14733175834026	21.791201356772987
18	22.5648761709973	27.400272062014075	28.061799696627045	21.973052070361586
19	22.41371928193816	27.488245913459664	28.23662318612602	21.861411618476158
20	22.322633523476043	27.526706000920342	28.18707279522965	21.963587680373966
21	22.442182870983547	27.52124780676551	28.05038462060964	21.986184701641303
22	22.376680657819243	27.523074705341898	28.185726611460755	21.9145180253781
23	22.37965556058441	27.605722624338974	28.05733728649267	21.957284528583944
24	22.345067791825016	27.58029933440369	28.016889440635467	22.057743433135826
25	22.317481127613476	27.763847972871936	27.926493738675838	21.99217716083875
26	22.415198237200517	27.6612183491853	27.903628155286064	22.019955258328114
27	22.40197762663099	27.588007802564967	27.84810055729897	22.161914013505072
28	22.411349771037536	27.608700240988743	27.885850194032326	22.0940997939414
29	22.39206796273265	27.723154787775684	27.838495712274447	22.04628153721722
30	22.46571192019216	27.631217018855182	27.72764855162754	22.175422509325124
31	22.56627207671569	27.570699132175264	27.671924056032744	22.1911047350763
32	22.59253231284031	27.6741952277401	27.55443404126462	22.178838418154974
33	22.53961103190848	27.611899950675177	27.558430789879534	22.290058227536804
34	22.596451293452784	27.606969713956982	27.548946698202187	22.24763229438805
35	22.56744508075849	27.589185430551705	27.50248769398323	22.340881794706576
36	22.641755412863247	27.58679110478603	27.489830657031167	22.28162282531956
37	22.59570447252015	27.56704588840367	27.55350784433886	22.28374179473732
38	22.61124094433841	27.627989384015216	27.601924266186238	22.158845405460134
39	22.59241954184405	27.452651558434617	27.605322117903608	22.349606781817723
40	22.64034498620942	27.498067652107604	27.58086834729887	22.280719014384108
41	22.604144165081273	27.537615239859765	27.565227376774544	22.293013218284422
42	22.581850127453958	27.49329461755185	27.546760452631386	22.378094802362806
43	22.63719178504728	27.557723296329133	27.437635631494267	22.367449287129322
44	22.745378795177743	27.54412143859933	27.383224242030817	22.32727552419211
45	22.70583718031408	27.489097448532736	27.40812785577257	22.396937515380618
46	22.569828614753977	27.581112123384816	27.41581451413894	22.433244747722267
47	22.575205798116613	27.569255226478667	27.428537794867026	22.427001180537694
48	22.653077187068536	27.47264633625036	27.366150421320206	22.508126055360894
49	22.714288685894683	27.634947104005597	27.307745417044956	22.343018793054767
50	22.561048170774274	27.568877081821018	27.43734266967188	22.432732077732823
51	22.63338632457526	27.519248740856394	27.41812890463966	22.429236029928685
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.83920198794234
2	41.73505698977713
3	42.854661690714856
4	43.49245291523732
5	44.47670519127702
6	43.59975841223567
7	44.431868555854734
8	43.14004716969694
9	43.218522499730774
10	43.18557930664152
11	44.752307929450474
12	43.2114745297907
13	44.333649324814196
14	44.49697426493036
15	44.361410557583994
16	44.463762482459444
17	44.42319832110721
18	44.537928241358884
19	44.27513090041432
20	44.286221203850005
21	44.42836757262486
22	44.291198683197344
23	44.33694008916836
24	44.40281122496084
25	44.309658288452226
26	44.43515349552863
27	44.56389164013606
28	44.50544956497893
29	44.43834949994987
30	44.64113442951728
31	44.75737681179199
32	44.77137073099529
33	44.82966925944528
34	44.844083587840835
35	44.908326875465065
36	44.923378238182806
37	44.879446267257464
38	44.77008634979855
39	44.94202632366177
40	44.921064000593525
41	44.8971573833657
42	44.959944929816764
43	45.0046410721766
44	45.07265431936985
45	45.1027746956947
46	45.00307336247624
47	45.00220697865431
48	45.16120324242943
49	45.05730747894945
50	44.9937802485071
51	45.06262235450394
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5996.0
1	4370.5
2	2745.0
3	4138.0
4	5531.0
5	9210.0
6	12889.0
7	15703.0
8	18517.0
9	16628.5
10	14740.0
11	17687.0
12	20634.0
13	28197.0
14	35760.0
15	56816.0
16	77872.0
17	136299.5
18	194727.0
19	242192.0
20	289657.0
21	329765.0
22	369873.0
23	424694.5
24	479516.0
25	580443.0
26	829059.0
27	976748.0
28	1033963.0
29	1091178.0
30	1116206.0
31	1141234.0
32	1216262.5
33	1291291.0
34	1365293.5
35	1439296.0
36	1649035.0
37	1858774.0
38	1923067.5
39	1987361.0
40	2086596.0
41	2185831.0
42	2141206.5
43	2096582.0
44	2074585.0
45	2052588.0
46	2005864.0
47	1959140.0
48	1923296.5
49	1887453.0
50	1813300.5
51	1739148.0
52	1630888.0
53	1522628.0
54	1427088.0
55	1331548.0
56	1240844.5
57	1150141.0
58	1030149.0
59	910157.0
60	824553.0
61	738949.0
62	652585.0
63	566221.0
64	500610.5
65	435000.0
66	370564.5
67	306129.0
68	303192.0
69	300255.0
70	269485.5
71	238716.0
72	203575.5
73	168435.0
74	141649.5
75	98903.5
76	82943.0
77	80018.5
78	77094.0
79	76657.0
80	76220.0
81	68353.5
82	60487.0
83	54305.5
84	48124.0
85	43236.0
86	38348.0
87	46006.0
88	53664.0
89	36581.5
90	19499.0
91	12771.0
92	6043.0
93	4660.0
94	3277.0
95	2507.5
96	1738.0
97	1320.5
98	903.0
99	559.5
100	216.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.5749757911965444E-4
2	0.0
3	0.0
4	0.0
5	0.0020113124668731863
6	0.002667242807692726
7	0.026815427108575124
8	0.0024496355856198928
9	0.00669608509064118
10	0.0032734343548956185
11	0.009549848374396335
12	0.027213337457508306
13	0.0048837277982345835
14	0.006161393059262219
15	0.008918787430385119
16	0.03240793271584693
17	0.003531454346781978
18	0.0011937310467995418
19	0.02341764576963789
20	0.005235008028152157
21	0.0011719703245922585
22	0.0240176199676387
23	0.001122231530975611
24	0.03099037709777248
25	0.003767713616461054
26	0.03142870021651919
27	0.03079763927250797
28	0.0030682618312269467
29	0.03753413713296268
30	0.0036868880768340015
31	0.003329390497714347
32	0.009963302096334718
33	0.011548726142865359
34	0.015391047949751385
35	0.017825140162366075
36	0.01694538525027162
37	0.021235356199707477
38	0.03148776503393896
39	0.020650925374711866
40	0.02391192503120332
41	0.02573671702201408
42	0.016957819948675783
43	0.022935801206476612
44	0.00867631081150396
45	0.02817702658383085
46	0.03952679755222962
47	0.020001212383094408
48	0.042790905883322114
49	0.020333840565405735
50	0.026128410021745176
51	0.099235110614414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.216805E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	42.508568947066394
#Duplication Level	Relative count
1	100.0
2	11.864441072331816
3	3.9076041139774067
4	2.0664306187826673
5	1.2395886022593154
6	0.8828190861574776
7	0.6771202158152082
8	0.5503287809812848
9	0.4336536840330467
10++	13.262518968133536
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	8450070	3.2454143	3.7414951	16
TGTGT	3846955	2.2889898	15.594852	4
TGTTT	4402405	2.1045454	12.984918	6
GTTTG	3174200	1.8886915	15.327982	7
TTTGA	3786440	1.8380966	12.852277	8
CTTTG	2906690	1.7757188	15.904836	1
TTTGT	3398935	1.6248422	12.5978155	2
GTGTT	2694150	1.6030554	15.182971	5
AACTT	3111255	1.5746733	5.008721	2
TGAGG	1908990	1.4356805	5.6913743	10
TTGTG	2403500	1.4301147	15.05629	3
TTGAG	2309915	1.3956987	9.195056	9
>>END_MODULE