##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_8_TGACCA_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	32168050	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59814797601968	34.0	31.0	34.0	31.0	34.0
2	32.876272730240096	34.0	31.0	34.0	31.0	34.0
3	32.96953996278916	34.0	31.0	34.0	31.0	34.0
4	36.41071681373288	37.0	37.0	37.0	35.0	37.0
5	36.32489000732093	37.0	37.0	37.0	35.0	37.0
6	36.31057123450132	37.0	37.0	37.0	35.0	37.0
7	36.30565203672588	37.0	37.0	37.0	35.0	37.0
8	36.283010067442696	37.0	37.0	37.0	35.0	37.0
9	38.02934874821445	39.0	38.0	39.0	35.0	39.0
10	38.010135429409	39.0	38.0	39.0	35.0	39.0
11	38.012467152967	39.0	38.0	39.0	35.0	39.0
12	37.98168822791559	39.0	38.0	39.0	35.0	39.0
13	37.97490426059397	39.0	38.0	39.0	35.0	39.0
14	39.487905079729735	41.0	39.0	41.0	37.0	41.0
15	39.488484008200686	41.0	39.0	41.0	37.0	41.0
16	39.477270210659334	41.0	39.0	41.0	37.0	41.0
17	39.4494590439893	41.0	39.0	41.0	37.0	41.0
18	39.40704618402421	41.0	39.0	41.0	36.0	41.0
19	39.41401974940974	41.0	39.0	41.0	37.0	41.0
20	39.38234216870466	41.0	39.0	41.0	36.0	41.0
21	39.35184821585393	41.0	39.0	41.0	36.0	41.0
22	39.32658404845802	41.0	39.0	41.0	36.0	41.0
23	39.30319621487781	40.0	39.0	41.0	36.0	41.0
24	39.263577804685085	41.0	39.0	41.0	36.0	41.0
25	39.210914774131474	40.0	39.0	41.0	36.0	41.0
26	39.1437522013302	40.0	39.0	41.0	36.0	41.0
27	39.056712980737096	40.0	39.0	41.0	36.0	41.0
28	39.01006821986412	40.0	39.0	41.0	36.0	41.0
29	38.924824321026605	40.0	39.0	41.0	35.0	41.0
30	38.83052124701373	40.0	38.0	41.0	35.0	41.0
31	38.77055326636212	40.0	38.0	41.0	35.0	41.0
32	38.7053480083499	40.0	38.0	41.0	35.0	41.0
33	38.62072177206887	40.0	38.0	41.0	35.0	41.0
34	38.76135911253557	40.0	38.0	41.0	35.0	41.0
35	38.7751573067065	40.0	38.0	41.0	35.0	41.0
36	38.74955193118638	40.0	38.0	41.0	35.0	41.0
37	38.718880783883385	40.0	38.0	41.0	35.0	41.0
38	38.67431625479319	40.0	38.0	41.0	35.0	41.0
39	38.581175731820856	40.0	38.0	41.0	35.0	41.0
40	38.52427187846326	40.0	38.0	41.0	35.0	41.0
41	38.478063730938	40.0	38.0	41.0	34.0	41.0
42	37.83237056644714	40.0	38.0	41.0	33.0	41.0
43	38.066573261357156	40.0	38.0	41.0	33.0	41.0
44	38.1646568878126	40.0	38.0	41.0	34.0	41.0
45	38.16002832002562	40.0	38.0	41.0	34.0	41.0
46	38.11324988614479	40.0	38.0	41.0	33.0	41.0
47	38.041307011149264	40.0	38.0	41.0	33.0	41.0
48	37.97164649395907	40.0	37.0	41.0	33.0	41.0
49	37.79083771008811	40.0	37.0	41.0	33.0	41.0
50	37.93794100046475	40.0	37.0	41.0	33.0	41.0
51	37.70970347907318	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	16.0
12	46.0
13	97.0
14	187.0
15	350.0
16	597.0
17	976.0
18	1715.0
19	3317.0
20	5799.0
21	10391.0
22	17315.0
23	27340.0
24	40634.0
25	58650.0
26	80337.0
27	108214.0
28	143012.0
29	187702.0
30	244359.0
31	316952.0
32	412109.0
33	550937.0
34	794902.0
35	1181133.0
36	1808062.0
37	3004728.0
38	6473068.0
39	1.6685906E7
40	9188.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.155740684176617	29.221061034432033	27.512017209967453	24.111181071423896
2	21.707315063898257	28.783209118023894	30.13728733286734	19.372188485210508
3	19.644153090922295	27.48695897854493	31.20685221303162	21.66203571750116
4	20.50711576926572	27.458408230520558	30.598955441981573	21.435520558232145
5	23.60517345627105	27.538921383173676	28.456909884186327	20.398995276368943
6	22.33334628614417	27.293046982953584	29.19094878303161	21.182657947870638
7	23.5019405901197	28.018235485209704	27.64643178557606	20.833392139094535
8	21.46784153842089	27.356398662648186	29.70393915702071	21.47182064191022
9	21.344623003259443	27.18419985047275	30.107096948680447	21.364080197587356
10	21.64186203391253	27.566622782543547	29.543425852670584	21.248089330873334
11	23.105797128753323	27.428223962737253	28.05384810861222	21.41213079989721
12	21.605689496254822	29.008394975760112	27.955915885482646	21.42999964250242
13	22.61559528787104	27.678690501911056	28.20573208509686	21.499982125121043
14	22.41235946847882	27.68971075337174	28.28820211358786	21.609727664561575
15	22.36809194216	27.603118622359762	28.244074477626093	21.784714957854145
16	22.346971606920533	27.566880802535437	28.264402100842297	21.821745489701737
17	22.42445971078353	27.60281967046557	28.166582150172363	21.806138468578535
18	22.355956461555365	27.581966914975233	28.12857570895216	21.933500914517243
19	22.208567196333007	27.66914065353666	28.272263938908328	21.850028211222007
20	22.141472672418754	27.69759124348538	28.22247851517266	21.9384575689232
21	22.270345886679486	27.614813456208875	28.118135852188743	21.9967048049229
22	22.21565186574878	27.623847264599497	28.241195844945526	21.919305024706194
23	22.27060079799677	27.657013713918005	28.110326861590927	21.9620586264943
24	22.221738650617613	27.661776203406795	28.063587938964286	22.052897207011306
25	22.206674013500972	27.82280243906609	27.979939722799486	21.990583824633447
26	22.31801741168644	27.714825735473553	27.94974516639958	22.01741168644043
27	22.304100242023836	27.666491564604794	27.869874402144795	22.159533791226572
28	22.31404452554631	27.65404492967401	27.950139968073913	22.081770576705768
29	22.335124448015968	27.78102496110271	27.87467067478445	22.009179916096873
30	22.406219836141762	27.66624026013389	27.795138343791432	22.132401559932916
31	22.483239544416527	27.621634930138605	27.76569744199045	22.129428083454417
32	22.493977386045962	27.710344148391787	27.674731034882388	22.120947430679863
33	22.428328724656097	27.679572175317517	27.65518765063716	22.236911449389225
34	22.468248469588247	27.686229693586263	27.659538552890663	22.185983283934828
35	22.47418319903138	27.67640522146797	27.576482354472848	22.272929225027802
36	22.56223406275971	27.701305596143026	27.521784649846175	22.214675691251077
37	22.490368548917328	27.65211444274676	27.610535919957847	22.246981088378064
38	22.50836155750815	27.714402955727813	27.633711089108605	22.143524397655437
39	22.503997677944348	27.57969872527861	27.641026659714424	22.27527693706261
40	22.55210371781939	27.57715186341727	27.6638776674371	22.20686675132624
41	22.5155317493307	27.594235883506883	27.64376724741748	22.24646511974494
42	22.636723525959663	27.66352567241937	27.49216875706021	22.207582044560755
43	22.548053356889667	27.63142318612862	27.512242604387616	22.308280852594095
44	22.671187715761445	27.582657326135717	27.475992483224815	22.270162474878024
45	22.625617654784794	27.494339880720155	27.51468304730936	22.365359417185683
46	22.5016468203699	27.638855945573326	27.544793669495043	22.31470356456173
47	22.532482636765568	27.658694655846368	27.526788106455662	22.282034600932402
48	22.567044399209575	27.53102499531393	27.490971481605992	22.410959123870505
49	22.73314049188558	27.7063048583921	27.352354276992237	22.208200372730087
50	22.51936191567819	27.593443329588496	27.526733960136923	22.360460794596392
51	22.570074418355286	27.579585147725577	27.493720206862015	22.35662022705712
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.266921755600514
2	41.079503549108765
3	41.30618880842346
4	41.942636327497866
5	44.00416873264
6	43.5160042340148
7	44.33533272921424
8	42.939662180331105
9	42.708703200846806
10	42.889951364785865
11	44.51792792865053
12	43.03568913875724
13	44.11557741299209
14	44.022087133040394
15	44.15280690001415
16	44.16871709662227
17	44.23059817936206
18	44.289457376072605
19	44.05859540755501
20	44.079930241341955
21	44.267050691602385
22	44.134956890454966
23	44.232659424491075
24	44.27463585762892
25	44.197257838134426
26	44.335429098126866
27	44.46363403325041
28	44.39581510225208
29	44.344304364112844
30	44.53862139607468
31	44.61266762787094
32	44.614924816725825
33	44.66524017404532
34	44.65423175352308
35	44.74711242405918
36	44.77690975401079
37	44.737349637295395
38	44.651885955163586
39	44.77927461500696
40	44.75897046914562
41	44.76199686907564
42	44.84430557052042
43	44.85633420948376
44	44.94135019063947
45	44.99097707197048
46	44.81635038493163
47	44.81451723769797
48	44.978003523080076
49	44.94134086461566
50	44.87982271027458
51	44.926694645412404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9577.0
1	6294.0
2	3011.0
3	4483.5
4	5956.0
5	6982.5
6	8009.0
7	12160.5
8	16312.0
9	14952.0
10	13592.0
11	16812.0
12	20032.0
13	28134.5
14	36237.0
15	59697.0
16	83157.0
17	144878.5
18	206600.0
19	257816.5
20	309033.0
21	350834.5
22	392636.0
23	447735.5
24	502835.0
25	612085.5
26	875065.0
27	1028794.0
28	1074624.5
29	1120455.0
30	1142344.5
31	1164234.0
32	1234532.0
33	1304830.0
34	1379684.5
35	1454539.0
36	1659616.0
37	1864693.0
38	1923890.0
39	1983087.0
40	2075734.5
41	2168382.0
42	2120231.5
43	2072081.0
44	2049036.0
45	2025991.0
46	1977978.5
47	1929966.0
48	1897807.0
49	1865648.0
50	1791125.0
51	1716602.0
52	1615458.5
53	1514315.0
54	1420207.5
55	1326100.0
56	1234393.0
57	1142686.0
58	1021801.5
59	900917.0
60	817354.0
61	733791.0
62	646046.5
63	558302.0
64	495300.5
65	432299.0
66	367139.5
67	301980.0
68	299964.0
69	297948.0
70	265894.0
71	233840.0
72	199185.5
73	164531.0
74	137840.5
75	94277.5
76	77405.0
77	76042.5
78	74680.0
79	74390.5
80	74101.0
81	65561.0
82	57021.0
83	49654.0
84	42287.0
85	38321.5
86	34356.0
87	41122.0
88	47888.0
89	30032.0
90	12176.0
91	6953.5
92	1731.0
93	1238.5
94	746.0
95	440.0
96	134.0
97	81.0
98	28.0
99	20.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026569841815092927
2	3.5749757911965444E-4
3	0.005775917408733199
4	0.04343440152573749
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.00650334726537667
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.008489790335441533
18	0.00420292806060672
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	4.4764914254982817E-4
28	0.0
29	0.0
30	0.0
31	2.1760722207283314E-4
32	3.8547565052901865E-4
33	3.3262818231133065E-4
34	2.2693324587595457E-4
35	7.398645550476327E-4
36	6.714737138247423E-4
37	0.0
38	0.0
39	3.108674601040473E-6
40	0.0
41	0.0017190970543753817
42	3.2330215850820925E-4
43	1.8030312686034746E-4
44	0.0
45	0.0
46	0.0
47	0.006515781963780832
48	0.011132163746325936
49	0.0
50	0.014461554244040283
51	0.036483405117810994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.216805E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	43.87398789283482
#Duplication Level	Relative count
1	100.0
2	12.40423277548359
3	4.156025863942621
4	2.0688437296239948
5	1.2517659204520757
6	0.9209954357748316
7	0.6669745707454902
8	0.5134753314496848
9	0.42925450988915453
10++	13.428466637687459
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	8230835	3.0880702	3.5615656	17
TGTGT	3534840	2.121318	16.526329	4
TGTTT	4050005	1.9217423	13.374826	6
GAGGG	1947560	1.8988957	5.3440356	11
CTTTG	2800400	1.7057039	16.546074	1
GTTTG	2819075	1.6917752	16.0382	7
TTTGA	3476995	1.6758116	13.283039	8
TTTGT	3298165	1.5649915	13.111228	2
GTGTT	2334095	1.4007304	15.93953	5
TGAGG	1815600	1.3996971	6.1443305	10
TTGAG	2111925	1.2873482	9.661171	9
TTGTG	2121140	1.2729324	15.889182	3
>>END_MODULE