FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_7_CTTGTA_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_7_CTTGTA_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 38770758
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 6933360 3.3250952 19.554623 4
TTTTT 11146495 3.0514638 3.6050115 16
TGTTT 6301130 2.2831452 14.806701 6
GTTTG 4350400 2.0863612 18.609718 7
GAGGG 2446990 2.0857687 6.5692225 11
CTTTG 4060580 2.0362453 19.677597 1
GTGTT 3997845 1.9172832 18.544123 5
TTTGA 5035740 1.8512357 14.503125 8
TTGTG 3859790 1.8510745 18.570986 3
TTTGT 5060015 1.8334407 14.475973 2
TGAGG 2777175 1.7885126 7.6262536 10
TTGAG 3255625 1.5840831 11.539115 9
ACTTT 3338680 1.2833792 5.2863927 3
AACTT 3155215 1.230531 5.542861 2
TAACT 2288635 0.89256555 5.327515 1
Produced by FastQC (version 0.10.1)