##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_7_CTTGTA_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	38770758	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31415413647574	34.0	31.0	34.0	31.0	34.0
2	32.46595702358979	34.0	31.0	34.0	31.0	34.0
3	32.523574003892314	34.0	31.0	34.0	31.0	34.0
4	35.96545324184789	37.0	37.0	37.0	35.0	37.0
5	35.969793342704314	37.0	37.0	37.0	35.0	37.0
6	35.98188872139152	37.0	37.0	37.0	35.0	37.0
7	35.97299820653494	37.0	37.0	37.0	35.0	37.0
8	35.99003217837526	37.0	37.0	37.0	35.0	37.0
9	37.73733350789789	39.0	38.0	39.0	35.0	39.0
10	37.72441725281719	39.0	38.0	39.0	35.0	39.0
11	37.69086173141108	39.0	38.0	39.0	35.0	39.0
12	37.65795835098194	39.0	38.0	39.0	35.0	39.0
13	37.65784457967007	39.0	38.0	39.0	35.0	39.0
14	39.16534510364745	41.0	39.0	41.0	36.0	41.0
15	39.132604939010996	41.0	39.0	41.0	36.0	41.0
16	39.08375007782927	41.0	39.0	41.0	36.0	41.0
17	39.07054342347395	41.0	39.0	41.0	36.0	41.0
18	39.03895925893427	41.0	39.0	41.0	36.0	41.0
19	39.05306625679075	41.0	39.0	41.0	36.0	41.0
20	39.018595457947974	41.0	39.0	41.0	36.0	41.0
21	38.99046817707304	41.0	39.0	41.0	36.0	41.0
22	38.94561700341273	41.0	39.0	41.0	36.0	41.0
23	38.91356049835291	41.0	39.0	41.0	36.0	41.0
24	38.84852901250989	41.0	39.0	41.0	35.0	41.0
25	38.81292031999993	41.0	39.0	41.0	35.0	41.0
26	38.771699743399395	41.0	39.0	41.0	35.0	41.0
27	38.717189976012335	40.0	39.0	41.0	35.0	41.0
28	38.666798957090286	40.0	39.0	41.0	35.0	41.0
29	38.56268443345885	40.0	39.0	41.0	35.0	41.0
30	38.54711775302407	40.0	39.0	41.0	34.0	41.0
31	38.505847706150085	40.0	38.0	41.0	34.0	41.0
32	38.45406331751368	40.0	38.0	41.0	34.0	41.0
33	38.40370242438902	40.0	38.0	41.0	34.0	41.0
34	38.33238627937065	40.0	38.0	41.0	34.0	41.0
35	38.293614249172016	40.0	38.0	41.0	34.0	41.0
36	38.244187333144225	40.0	38.0	41.0	34.0	41.0
37	38.184853749828676	40.0	38.0	41.0	34.0	41.0
38	38.11538198453587	40.0	38.0	41.0	33.0	41.0
39	38.064658575929826	40.0	38.0	41.0	33.0	41.0
40	37.98915574464652	40.0	38.0	41.0	33.0	41.0
41	38.179301524102264	40.0	38.0	41.0	34.0	41.0
42	38.218281210803255	40.0	38.0	41.0	34.0	41.0
43	38.20428463121613	40.0	38.0	41.0	34.0	41.0
44	38.16188891638384	40.0	38.0	41.0	33.0	41.0
45	38.13030993616375	40.0	38.0	41.0	33.0	41.0
46	38.07001743427353	40.0	38.0	41.0	33.0	41.0
47	38.0254910672626	40.0	38.0	41.0	33.0	41.0
48	37.961654683150634	40.0	38.0	41.0	33.0	41.0
49	37.90410538272169	40.0	38.0	41.0	33.0	41.0
50	37.80829064523319	40.0	38.0	41.0	33.0	41.0
51	36.34525257411784	38.0	36.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	20.0
5	101.0
6	816.0
7	3909.0
8	12690.0
9	19775.0
10	22532.0
11	25704.0
12	33202.0
13	42350.0
14	51090.0
15	57571.0
16	60427.0
17	62003.0
18	63141.0
19	64365.0
20	67649.0
21	72298.0
22	78360.0
23	86513.0
24	97766.0
25	111422.0
26	127733.0
27	148979.0
28	176321.0
29	212984.0
30	260540.0
31	327495.0
32	424892.0
33	569743.0
34	809419.0
35	1187625.0
36	1819433.0
37	3095136.0
38	7360088.0
39	2.1212088E7
40	4576.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.87150992258364	30.39333510825244	27.61385254033277	22.121302428831154
2	21.78690085757616	30.336964668757687	29.97924856610875	17.896885907557394
3	21.49703657781301	28.51615378988616	30.36102557404408	19.62578405825675
4	21.310939216829247	27.765497567744628	30.597019390981828	20.326543824444297
5	23.819142292363292	28.07166210788239	28.756416061984197	19.35277953777012
6	21.46581709854628	27.629728054323827	30.583554750206325	20.320900096923562
7	23.070796873399722	27.98474158389201	29.16775770215157	19.7767038405567
8	21.066442832918955	27.82507096514401	30.80573339865457	20.30275280328246
9	21.36562644355361	27.653811414859415	30.811411996329163	20.169150145257813
10	21.3863911246198	28.510964909509028	30.104348400721403	19.998295565149764
11	23.05255424858586	27.841633311725815	29.05107052657944	20.054741913108884
12	20.99423183026016	29.663459536566094	29.152481356295663	20.18982727687808
13	22.20609690297908	27.985675990614656	29.456407775195043	20.351819331211217
14	22.33023625189692	28.167301518320002	29.01154433313647	20.490917896646604
15	22.001259005493996	28.59032784054248	28.77315318454748	20.635259969416044
16	22.1685400199764	28.191410435902693	28.869088707851486	20.770960836269417
17	22.081406300170617	28.242278007017802	28.91024623790024	20.766069454911346
18	21.981641526618763	28.186805942160625	28.923006600062152	20.90854593115846
19	21.85654044799056	28.326422704959846	29.011566041211132	20.805470805838464
20	21.718166603537085	28.366739133490753	29.014274507012157	20.900819755960008
21	21.786215493873843	28.34661825590379	28.92741947610841	20.939746774113964
22	21.718786649967136	28.433212840667654	28.94326171021433	20.90473879915088
23	21.744516764341217	28.442233756983942	28.88382182731953	20.929427651355315
24	21.67466773614918	28.467328164592367	28.826641865356205	21.031362233902247
25	21.696328542950635	28.548454181741434	28.75834756308696	20.996869712220974
26	21.710376931747827	28.53648168225622	28.74759343004823	21.005547955947726
27	21.716071983890934	28.502714038942077	28.672896861461535	21.108317115705454
28	21.699678344054853	28.518853512306684	28.667129535907033	21.114338607731423
29	21.73462027819318	28.578167034308766	28.61926907609142	21.067943611406633
30	21.742873652065207	28.497058979807548	28.591227676611208	21.168839691516038
31	21.832534699010793	28.50205942677325	28.509765717162423	21.155640157053526
32	21.80433722863682	28.532395823985873	28.49453812445531	21.168728822922
33	21.79925926338696	28.50490587603027	28.454039698578747	21.241795162004014
34	21.830951972660635	28.538421814724863	28.417496440692457	21.21312977192204
35	21.80420367621757	28.52839862336594	28.426489835948182	21.2409078644683
36	21.820419219500103	28.50674801855671	28.40153622070978	21.271296541233404
37	21.820107941663387	28.49922569174723	28.405152520784615	21.275513845804767
38	21.79750921383197	28.510717845624438	28.46263905907097	21.229133881472617
39	21.788951302185204	28.456125821408087	28.438504042819346	21.316418833587363
40	21.812846348971917	28.47286631487661	28.405062742429767	21.309224593721705
41	21.799849696167705	28.4739419527549	28.419339952989553	21.306868398087843
42	21.78530222208326	28.435598674243774	28.401829431321822	21.377269672351147
43	21.833334889989235	28.47677676661653	28.333661607122167	21.356226736272063
44	21.824360777894185	28.49674028744697	28.32793455063147	21.350964384027378
45	21.8444592725282	28.448804469836464	28.334898504025695	21.37183775360964
46	21.79427512907362	28.496409809827178	28.313978332382224	21.395336728716984
47	21.812257604836816	28.471271605525967	28.32806664269726	21.38840414693996
48	21.794654847218215	28.450294868984184	28.307079242805504	21.447971040992098
49	21.817648343232577	28.503080430915972	28.2683927242787	21.41087850157275
50	21.791750739520126	28.49004681884879	28.303087931216353	21.415114510414725
51	21.8111214611591	28.437686885830338	28.311690518666193	21.43950113434437
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.99281235141479
2	39.683786765133554
3	41.122820636069754
4	41.63748304127354
5	43.171921830133414
6	41.78671719546984
7	42.84750071395642
8	41.36919563620141
9	41.534776588811425
10	41.384686689769566
11	43.10729616169475
12	41.184059107138246
13	42.5579162341903
14	42.82115414854352
15	42.63651897491004
16	42.93950085624582
17	42.84747575508196
18	42.89018745777722
19	42.66201125382902
20	42.61898635949709
21	42.7259622679878
22	42.62352544911801
23	42.67394441569653
24	42.70602997005143
25	42.6931982551716
26	42.71592488769555
27	42.82438909959639
28	42.81401695178628
29	42.80256388959982
30	42.91171334358124
31	42.98817485606432
32	42.97306605155882
33	43.041054425390975
34	43.04408174458268
35	43.04511154068587
36	43.09171576073351
37	43.095621787468154
38	43.02664309530459
39	43.10537013577257
40	43.122070942693625
41	43.10671809425555
42	43.16257189443441
43	43.189561626261295
44	43.17532516192156
45	43.216297026137845
46	43.1896118577906
47	43.20066175177678
48	43.24262588821031
49	43.22852684480532
50	43.20686524993485
51	43.250622595503465
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12838.0
1	8931.0
2	5024.0
3	6795.5
4	8567.0
5	15629.5
6	22692.0
7	28163.0
8	33634.0
9	30347.0
10	27060.0
11	32456.5
12	37853.0
13	50037.5
14	62222.0
15	85618.0
16	109014.0
17	157670.5
18	206327.0
19	260884.0
20	315441.0
21	380026.0
22	444611.0
23	531618.0
24	618625.0
25	734044.0
26	993655.0
27	1137847.0
28	1253367.5
29	1368888.0
30	1478013.0
31	1587138.0
32	1711944.5
33	1836751.0
34	1955000.5
35	2073250.0
36	2242476.0
37	2411702.0
38	2499894.0
39	2588086.0
40	2669401.0
41	2750716.0
42	2764878.0
43	2779040.0
44	2767093.5
45	2755147.0
46	2692641.0
47	2630135.0
48	2534556.0
49	2438977.0
50	2301674.5
51	2164372.0
52	1999325.5
53	1834279.0
54	1665843.0
55	1497407.0
56	1336484.0
57	1175561.0
58	1028646.0
59	881731.0
60	761243.5
61	640756.0
62	540115.5
63	439475.0
64	368359.0
65	297243.0
66	245595.0
67	193947.0
68	169326.0
69	144705.0
70	125243.0
71	105781.0
72	89456.0
73	73131.0
74	61022.0
75	41305.0
76	33697.0
77	31143.0
78	28589.0
79	27695.0
80	26801.0
81	23481.5
82	20162.0
83	17606.5
84	15051.0
85	13354.5
86	11658.0
87	13639.5
88	15621.0
89	10848.0
90	6075.0
91	4148.5
92	2222.0
93	1750.0
94	1278.0
95	998.5
96	719.0
97	564.5
98	410.0
99	268.0
100	126.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002362605342923654
2	0.0023393919716503866
3	1.0832906594191425E-4
4	4.0752362901958227E-4
5	0.004255784733432346
6	0.0
7	0.020345230289281424
8	8.150472580391645E-4
9	0.003649657816852588
10	0.0033762558885229946
11	0.00873854465264775
12	0.024286344878787253
13	0.014531570417065357
14	0.006824731154340598
15	0.005321020548527836
16	0.049098859506435236
17	0.00785643654426359
18	5.519623836088013E-4
19	0.03059264407469155
20	0.0033349876729260744
21	0.0022362214326580874
22	0.022661408889658542
23	0.0070336514958000045
24	0.08516727993814306
25	0.035949774311866696
26	0.02764196665951179
27	0.0654281765654414
28	0.009981749647504957
29	0.09772571379698071
30	0.05181482394540751
31	0.026347176395158434
32	0.03213762289610123
33	0.02130987482885942
34	0.03771914905558463
35	0.00964128686883037
36	0.010814851749867774
37	0.013383798170776026
38	0.051017831531692
39	0.016058494394151388
40	0.037884221917972305
41	0.01704893156847746
42	0.01979326790567262
43	0.03213762289610123
44	0.011098570732096597
45	0.032168574057798924
46	0.03261220737546581
47	0.033589748232417845
48	0.057241594296402465
49	0.006816993363916177
50	0.09157417040956486
51	0.13049525624440977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.8770758E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	23.526462315733177
#Duplication Level	Relative count
1	100.0
2	10.989484463982416
3	3.1228144669797184
4	1.5211309821160954
5	0.8829553401938256
6	0.6188180637426316
7	0.41712458787091616
8	0.3715406134478969
9	0.310345688880008
10++	6.653386951743431
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	6933360	3.3250952	19.554623	4
TTTTT	11146495	3.0514638	3.6050115	16
TGTTT	6301130	2.2831452	14.806701	6
GTTTG	4350400	2.0863612	18.609718	7
GAGGG	2446990	2.0857687	6.5692225	11
CTTTG	4060580	2.0362453	19.677597	1
GTGTT	3997845	1.9172832	18.544123	5
TTTGA	5035740	1.8512357	14.503125	8
TTGTG	3859790	1.8510745	18.570986	3
TTTGT	5060015	1.8334407	14.475973	2
TGAGG	2777175	1.7885126	7.6262536	10
TTGAG	3255625	1.5840831	11.539115	9
ACTTT	3338680	1.2833792	5.2863927	3
AACTT	3155215	1.230531	5.542861	2
TAACT	2288635	0.89256555	5.327515	1
>>END_MODULE