FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_7_CTTGTA_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_7_CTTGTA_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 38770758
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 6204905 3.072726 20.876217 4
TGTTT 5680465 2.0873902 15.498021 6
GAGGG 2200140 2.009225 7.037576 11
CTTTG 3786795 1.9287997 20.546513 1
GTTTG 3717355 1.8408684 19.811008 7
TTTGT 4795080 1.7620391 15.191436 2
TGAGG 2571190 1.7423822 8.272558 10
GTGTT 3372150 1.6699197 19.767817 5
TTTGA 4429815 1.6529392 15.192323 8
TTGTG 3320995 1.644587 19.83414 3
TTGAG 2879875 1.4481511 12.349365 9
Produced by FastQC (version 0.10.1)