##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_7_CTTGTA_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	38770758	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.509973805516005	34.0	31.0	34.0	31.0	34.0
2	32.843056537610124	34.0	31.0	34.0	31.0	34.0
3	32.97458772923655	34.0	31.0	34.0	31.0	34.0
4	36.44243506923439	37.0	37.0	37.0	35.0	37.0
5	36.365001272350675	37.0	37.0	37.0	35.0	37.0
6	36.34795592595842	37.0	37.0	37.0	35.0	37.0
7	36.33613980412763	37.0	37.0	37.0	35.0	37.0
8	36.33529948008754	37.0	37.0	37.0	35.0	37.0
9	38.10836504666739	39.0	38.0	39.0	37.0	39.0
10	38.105940745342146	39.0	38.0	39.0	37.0	39.0
11	38.085658371703744	39.0	38.0	39.0	37.0	39.0
12	38.077272670294455	39.0	38.0	39.0	37.0	39.0
13	38.063880541102655	39.0	38.0	39.0	37.0	39.0
14	39.60485585553937	41.0	40.0	41.0	37.0	41.0
15	39.60348807211868	41.0	40.0	41.0	37.0	41.0
16	39.59280208037202	41.0	40.0	41.0	37.0	41.0
17	39.54604171009502	41.0	40.0	41.0	37.0	41.0
18	39.52840393267524	41.0	40.0	41.0	37.0	41.0
19	39.540812846630445	41.0	40.0	41.0	37.0	41.0
20	39.517480003873025	41.0	40.0	41.0	37.0	41.0
21	39.50438314876382	41.0	39.0	41.0	37.0	41.0
22	39.475949116083825	41.0	39.0	41.0	37.0	41.0
23	39.44563402655166	41.0	39.0	41.0	37.0	41.0
24	39.424575939423214	41.0	39.0	41.0	37.0	41.0
25	39.38140002318242	41.0	39.0	41.0	36.0	41.0
26	39.3176512566507	41.0	39.0	41.0	36.0	41.0
27	39.25751794690215	40.0	39.0	41.0	36.0	41.0
28	39.19069833506995	40.0	39.0	41.0	36.0	41.0
29	39.13297746203466	40.0	39.0	41.0	36.0	41.0
30	39.077523116777854	40.0	39.0	41.0	36.0	41.0
31	39.0164168314687	40.0	39.0	41.0	36.0	41.0
32	32.46041158132632	40.0	35.0	41.0	2.0	41.0
33	38.88614870516589	40.0	38.0	41.0	35.0	41.0
34	38.999775500907155	40.0	39.0	41.0	36.0	41.0
35	39.02866931309416	40.0	39.0	41.0	36.0	41.0
36	39.02119079023423	40.0	39.0	41.0	36.0	41.0
37	38.98620106937295	40.0	39.0	41.0	35.0	41.0
38	38.941304526468116	40.0	39.0	41.0	35.0	41.0
39	38.81646997977187	40.0	39.0	41.0	35.0	41.0
40	38.711298680309525	40.0	38.0	41.0	35.0	41.0
41	38.738987409015834	40.0	38.0	41.0	35.0	41.0
42	38.74223201929661	40.0	38.0	41.0	35.0	41.0
43	38.700908736424495	40.0	38.0	41.0	35.0	41.0
44	38.62919386306556	40.0	38.0	41.0	35.0	41.0
45	38.61650497006017	40.0	38.0	41.0	35.0	41.0
46	38.54206363465992	40.0	38.0	41.0	35.0	41.0
47	38.48574941454588	40.0	38.0	41.0	35.0	41.0
48	38.4279011774802	40.0	38.0	41.0	34.0	41.0
49	38.39363127746948	40.0	38.0	41.0	34.0	41.0
50	38.22298436878639	40.0	38.0	41.0	34.0	41.0
51	38.073476794031215	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	9.0
11	33.0
12	57.0
13	115.0
14	199.0
15	330.0
16	537.0
17	877.0
18	1588.0
19	2862.0
20	5524.0
21	10033.0
22	17256.0
23	27361.0
24	41544.0
25	60327.0
26	83088.0
27	112920.0
28	149902.0
29	198884.0
30	259129.0
31	336866.0
32	439103.0
33	591169.0
34	842904.0
35	1247170.0
36	1948583.0
37	3574203.0
38	9646322.0
39	1.9162622E7
40	9237.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.884369258768622	30.908759369271664	27.523951476942816	22.68291989501689
2	20.48135349842786	29.723628307705514	31.075949043864448	18.719069150002174
3	19.525852220508863	28.124108652411156	32.17280059974393	20.17723852733605
4	20.242790228440747	28.30704057038165	31.539692402447738	19.91047679872987
5	23.45482644419797	28.48159171920239	28.780489666980458	19.283092169619177
6	21.619648873963214	28.097186340192735	29.954717002739812	20.328447783104238
7	23.132797661577833	28.550556065991795	28.40867852003306	19.907967752397308
8	20.629134978480433	28.200121854723605	30.652730596600662	20.518012570195303
9	20.62436334105204	28.02965833167358	30.948592751268883	20.397385576005505
10	20.859236231594956	28.29914751731189	30.610518370571967	20.23109788052119
11	22.790537003445298	28.199977653142522	28.804712401309118	20.20477294210307
12	20.77655536164653	30.125792227224444	28.735796189488994	20.361856221640032
13	21.96541011656259	28.40031396858426	29.168539856765246	20.465736058087902
14	21.62654557539568	28.437738702792863	29.309911555758973	20.62580416605248
15	21.76075329762704	28.39679327394115	29.11164904230142	20.73080438613039
16	21.720658646911158	28.357962462327922	29.06633138304905	20.85504750771187
17	21.79130908525281	28.440490602258716	28.955784454665828	20.812415857822646
18	21.63514858744144	28.368218654312976	29.04961116804858	20.947021590197007
19	21.50402247963458	28.53609927507955	29.118928020377844	20.840950224908028
20	21.398234717069332	28.514393055576083	29.166458711247977	20.92091351610661
21	21.526894057629722	28.46904360239746	29.021648222611486	20.982414117361337
22	21.494127094445766	28.518160516748214	29.030505413383974	20.957206975422043
23	21.554528286498808	28.534789028370298	28.93621012000849	20.974472565122404
24	21.47528041623535	28.524425547728523	28.89923637809712	21.101057657939005
25	21.522150792099552	28.594906501441113	28.85743167569744	21.025511030761894
26	21.557058543967596	28.55712029153518	28.815428370010203	21.07039279448702
27	21.556075844583695	28.530700895762727	28.745171812219922	21.168051447433655
28	21.5473115072963	28.573477980492413	28.76778937363051	21.111421138580784
29	21.599517174466765	28.589094609786013	28.723040573109515	21.08834764263771
30	21.58038798209723	28.531601058715438	28.70537119754017	21.182639761647167
31	21.651929013097966	28.530007073888008	28.620405100153057	21.19765881286097
32	21.661544727870275	28.594204028944393	28.585053279192753	21.159197963992582
33	21.663695613070036	28.53068542018188	28.538972593726434	21.266646373021647
34	21.64721488058862	28.566128468014284	28.546507694466772	21.240148956930327
35	21.65204572552131	28.551291097909488	28.56702207128558	21.22964110528362
36	21.686377158516677	28.530760917993735	28.526239464924075	21.256622458565513
37	21.734364853016285	28.53933884913986	28.480531641914247	21.245764655929605
38	21.67126833063207	28.53675958566505	28.57491720951135	21.217054874191525
39	21.71660146546529	28.567202632458205	28.468496798540798	21.247699103535712
40	21.73298494705726	28.58712744280109	28.43886106121526	21.241026548926385
41	21.673056688141777	28.496922621764796	28.534604067083137	21.295416623010286
42	21.64793020385487	28.454656640561417	28.49561657743565	21.401796578148062
43	21.72426445724894	28.48945589353708	28.44987193698921	21.336407712224766
44	21.743211726734877	28.483619020293595	28.41903684214789	21.35413241082364
45	21.73940742995246	28.43087069674827	28.45837127064413	21.37135060265514
46	21.690967104478823	28.4762129192649	28.47301706575457	21.35980291050171
47	21.701830566962734	28.505967432068797	28.445161529817785	21.34704047115068
48	21.70862068129465	28.432694520938163	28.43925628399723	21.419428513769954
49	21.736523909668442	28.47803819967464	28.389455595622582	21.395982295034337
50	21.67694566358929	28.60434981249118	28.33414208362452	21.38456244029501
51	21.70909160199188	28.420609976967476	28.399670378182577	21.470628042858067
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.56728915378552
2	39.200422648430035
3	39.70309074784492
4	40.15326702717062
5	42.73791861381714
6	41.94809665706745
7	43.04076541397514
8	41.14714754867573
9	41.02174891705754
10	41.090334112116146
11	42.995309945548364
12	41.13841158328656
13	42.43114617465049
14	42.25234974144816
15	42.491557683757435
16	42.57570615462303
17	42.603724943075456
18	42.582170177638446
19	42.344972704542606
20	42.31914823317594
21	42.50930817499106
22	42.45133406986781
23	42.52900085162121
24	42.57633807417436
25	42.54766182286145
26	42.62745133845461
27	42.72412729201735
28	42.65873264587708
29	42.687864817104476
30	42.763027743744395
31	42.849587825958935
32	42.820742691862854
33	42.93034198609168
34	42.88736383751895
35	42.88168683080493
36	42.94299961708219
37	42.98012950894589
38	42.88832320482359
39	42.964300569001
40	42.974011495983646
41	42.96847331115207
42	43.049726782002935
43	43.060672169473705
44	43.097344137558515
45	43.110758032607606
46	43.050770014980536
47	43.04887103811342
48	43.128049195064605
49	43.13250620470278
50	43.0615081038843
51	43.179719644849946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18882.0
1	12085.5
2	5289.0
3	7264.0
4	9239.0
5	12062.0
6	14885.0
7	23386.5
8	31888.0
9	29195.5
10	26503.0
11	32728.5
12	38954.0
13	53316.5
14	67679.0
15	95263.0
16	122847.0
17	175800.0
18	228753.0
19	289262.5
20	349772.0
21	418732.0
22	487692.0
23	579708.0
24	671724.0
25	794519.5
26	1061072.0
27	1204829.0
28	1311272.5
29	1417716.0
30	1522816.0
31	1627916.0
32	1749361.0
33	1870806.0
34	1987174.5
35	2103543.0
36	2262447.0
37	2421351.0
38	2505987.5
39	2590624.0
40	2664349.5
41	2738075.0
42	2747361.5
43	2756648.0
44	2740610.0
45	2724572.0
46	2661657.0
47	2598742.0
48	2499592.5
49	2400443.0
50	2263465.0
51	2126487.0
52	1958651.5
53	1790816.0
54	1623822.0
55	1456828.0
56	1296717.5
57	1136607.0
58	991128.0
59	845649.0
60	728871.0
61	612093.0
62	514227.5
63	416362.0
64	347642.0
65	278922.0
66	230135.5
67	181349.0
68	158267.5
69	135186.0
70	116816.5
71	98447.0
72	82556.5
73	66666.0
74	54890.0
75	36130.0
76	29146.0
77	27422.5
78	25699.0
79	25005.0
80	24311.0
81	20930.5
82	17550.0
83	14933.5
84	12317.0
85	11175.5
86	10034.0
87	11213.5
88	12393.0
89	7874.0
90	3355.0
91	1924.5
92	494.0
93	331.0
94	168.0
95	113.5
96	59.0
97	38.0
98	17.0
99	9.5
100	2.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.02443594216032609
2	0.0
3	0.005705330806274151
4	0.030608119655540392
5	0.0
6	0.0014882350249639175
7	0.0
8	0.0
9	0.0
10	0.0
11	5.313282758103414E-4
12	0.0
13	0.0
14	7.737790424422447E-6
15	0.0
16	0.0
17	0.01167890501392828
18	3.946273116455448E-4
19	5.416453297095713E-5
20	0.0011065040306924101
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0016662042047256337
30	0.0
31	0.0
32	17.526704017496897
33	0.0
34	0.0016120396717546764
35	2.88877509178438E-4
36	9.285348509306938E-5
37	0.0
38	0.0
39	0.0
40	0.0
41	0.029994254948536213
42	0.003010000475100332
43	0.0
44	0.0
45	0.0016971553664233236
46	0.004570454877358859
47	0.016582084879537305
48	0.001779691797617163
49	4.281577368180421E-4
50	0.018694501665404633
51	0.10397526919643924
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.8770758E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	23.206355401758298
#Duplication Level	Relative count
1	100.0
2	10.580469210028822
3	2.974556047974711
4	1.3822171258561378
5	0.8008181733659776
6	0.5776799826448074
7	0.4512350079028109
8	0.3185917500852264
9	0.3092943254718443
10++	6.570799888430905
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	6204905	3.072726	20.876217	4
TGTTT	5680465	2.0873902	15.498021	6
GAGGG	2200140	2.009225	7.037576	11
CTTTG	3786795	1.9287997	20.546513	1
GTTTG	3717355	1.8408684	19.811008	7
TTTGT	4795080	1.7620391	15.191436	2
TGAGG	2571190	1.7423822	8.272558	10
GTGTT	3372150	1.6699197	19.767817	5
TTTGA	4429815	1.6529392	15.192323	8
TTGTG	3320995	1.644587	19.83414	3
TTGAG	2879875	1.4481511	12.349365	9
>>END_MODULE