FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_6_ATCACG_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_6_ATCACG_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 41546241
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11504325 3.0968528 3.6756175 16
TGTGT 6722635 3.0274105 18.719046 4
TGTTT 6455960 2.2477937 14.683729 6
GTTTG 4525670 2.0380495 18.06716 7
GAGGG 2653335 2.035002 6.072725 11
CTTTG 4236340 1.9801956 18.876415 1
TTTGA 5247215 1.8599147 14.461786 8
GTGTT 4066685 1.8313543 17.946077 5
TTTGT 5161080 1.7969507 14.319666 2
TTGTG 3895975 1.7544783 17.925306 3
TGAGG 2901795 1.7206925 7.2098837 10
TTGAG 3376945 1.5481904 11.14625 9
ACTTT 3539150 1.3021128 5.313024 3
AACTT 3459045 1.2956101 5.62831 2
TAACT 2452245 0.91850626 5.3407955 1
Produced by FastQC (version 0.10.1)