##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_6_ATCACG_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	41546241	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17620852870901	33.0	31.0	34.0	31.0	34.0
2	32.35062096231522	34.0	31.0	34.0	31.0	34.0
3	32.40665635670866	34.0	31.0	34.0	31.0	34.0
4	35.88371937186808	37.0	35.0	37.0	35.0	37.0
5	35.898270387446125	37.0	37.0	37.0	35.0	37.0
6	35.916183873289526	37.0	37.0	37.0	35.0	37.0
7	35.93337402052811	37.0	37.0	37.0	35.0	37.0
8	35.94540475996372	37.0	37.0	37.0	35.0	37.0
9	37.68257441148526	39.0	38.0	39.0	35.0	39.0
10	37.66803704335128	39.0	38.0	39.0	35.0	39.0
11	37.63100820601315	39.0	38.0	39.0	35.0	39.0
12	37.610170003105694	39.0	38.0	39.0	35.0	39.0
13	37.59733399226178	39.0	38.0	39.0	35.0	39.0
14	39.067934882484316	41.0	39.0	41.0	36.0	41.0
15	39.03322228357555	41.0	39.0	41.0	36.0	41.0
16	38.99130838335049	41.0	39.0	41.0	36.0	41.0
17	38.981852870877056	41.0	39.0	41.0	36.0	41.0
18	38.92491111289707	41.0	39.0	41.0	36.0	41.0
19	38.942790684721636	41.0	39.0	41.0	36.0	41.0
20	38.9280964070853	41.0	39.0	41.0	36.0	41.0
21	38.8891168517508	41.0	39.0	41.0	36.0	41.0
22	38.820659539331125	40.0	39.0	41.0	35.0	41.0
23	38.795970350241795	40.0	39.0	41.0	35.0	41.0
24	38.75240973064206	40.0	39.0	41.0	35.0	41.0
25	38.712516518642445	40.0	39.0	41.0	35.0	41.0
26	38.65708433164868	40.0	39.0	41.0	35.0	41.0
27	38.6138176014528	40.0	39.0	41.0	35.0	41.0
28	38.545166240190056	40.0	38.0	41.0	34.0	41.0
29	38.48393911256616	40.0	38.0	41.0	34.0	41.0
30	38.447432993998184	40.0	38.0	41.0	34.0	41.0
31	38.395605296758376	40.0	38.0	41.0	34.0	41.0
32	38.336186756342165	40.0	38.0	41.0	34.0	41.0
33	38.2820176439067	40.0	38.0	41.0	34.0	41.0
34	38.230079034105636	40.0	38.0	41.0	34.0	41.0
35	38.179026569455466	40.0	38.0	41.0	34.0	41.0
36	38.11233827387657	40.0	38.0	41.0	33.0	41.0
37	38.04242699116871	40.0	38.0	41.0	33.0	41.0
38	37.97434225156495	40.0	38.0	41.0	33.0	41.0
39	37.91422865428427	40.0	38.0	41.0	33.0	41.0
40	37.81626289608246	40.0	38.0	41.0	33.0	41.0
41	38.01211546430879	40.0	38.0	41.0	33.0	41.0
42	38.050872231738126	40.0	38.0	41.0	33.0	41.0
43	38.03967198861625	40.0	38.0	41.0	33.0	41.0
44	37.99826619693464	40.0	38.0	41.0	33.0	41.0
45	37.944824707486774	40.0	38.0	41.0	33.0	41.0
46	37.89728353523006	40.0	38.0	41.0	33.0	41.0
47	37.84022737460171	40.0	38.0	41.0	33.0	41.0
48	37.774146185692224	40.0	38.0	41.0	33.0	41.0
49	37.705640902627025	40.0	37.0	41.0	33.0	41.0
50	37.648836220826816	40.0	37.0	41.0	33.0	41.0
51	36.12833322273368	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	27.0
6	502.0
7	2944.0
8	9527.0
9	14452.0
10	17691.0
11	22514.0
12	31501.0
13	42925.0
14	53090.0
15	59952.0
16	63804.0
17	66144.0
18	68262.0
19	70668.0
20	74997.0
21	80475.0
22	88769.0
23	99235.0
24	112629.0
25	129320.0
26	149415.0
27	174760.0
28	208613.0
29	252857.0
30	311024.0
31	391095.0
32	508297.0
33	684788.0
34	974535.0
35	1424843.0
36	2165473.0
37	3621721.0
38	8272774.0
39	2.1293124E7
40	3490.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.528828067360728	29.145442456366794	28.37331477595928	22.952414700313202
2	22.326758755382947	29.68628858625261	29.629932103845448	18.357020554519
3	21.523896710655485	28.159953147145128	29.998157956095234	20.317992186104153
4	21.465679650777552	27.236557935530197	30.242136707385875	21.055625706306376
5	23.93484429866552	27.480981350404456	28.71700733209846	19.867167018831566
6	21.846348089124227	27.16720014698239	30.385443890837415	20.60100787305597
7	23.38198768897304	27.681902852004743	28.77001515759291	20.16609430142931
8	21.52223461996054	27.40476327332257	30.45504016202878	20.617961944688115
9	21.596438385003676	27.290440183267616	30.58762569776124	20.52549573396747
10	21.71528823717741	28.043265766838633	29.783693551225454	20.457752444758505
11	23.30056078652957	27.444625029268437	28.692748954562795	20.5620652296392
12	21.365801651715017	29.254562351183843	28.706704862025756	20.67293113507538
13	22.513667307999533	27.668465176458167	29.016181292559935	20.801686222982365
14	22.648353664504455	27.832752955100325	28.61552579052012	20.9033675898751
15	22.249127535816243	28.25910871335698	28.42942870431176	21.062335046515017
16	22.409499560815924	27.86894490049876	28.55224024809945	21.169315290585857
17	22.328975381967638	27.880550490735896	28.614132953020537	21.17634117427593
18	22.25246947731217	27.82732812405413	28.605915865972765	21.314286532660933
19	22.099849411488037	27.97667268412412	28.708359173138128	21.215118731249717
20	21.998258090032767	27.989385147375966	28.701275246303005	21.31108151628826
21	22.078939451338705	27.989822109563526	28.593058431669327	21.338180007428438
22	21.998982188001115	28.072033046484464	28.63420588875842	21.294778876756006
23	22.014794345095172	28.107599674662183	28.565292821003858	21.312313159238787
24	21.962637884290814	28.117994007395623	28.503138959166012	21.416229149147547
25	21.962207399077233	28.209015651597895	28.438342467283384	21.39043448204149
26	22.010115528441784	28.180116445303604	28.4098519691919	21.399916057062708
27	22.002126351569167	28.136660500261563	28.359961845383463	21.501251302785803
28	22.005894337122964	28.176611555288535	28.331179040730813	21.48631506685769
29	22.01268213988832	28.195800215904633	28.31292894961478	21.47858869459227
30	22.031598228326324	28.144483320135087	28.266308812506903	21.557609639031682
31	22.113874754494155	28.134757831274143	28.20578952339588	21.54557789083582
32	22.130531712174097	28.18302893769572	28.132241835097037	21.554197515033145
33	22.099131686422453	28.146177027701736	28.113354293503708	21.641336992372104
34	22.136097471763367	28.164642231898256	28.087845897361476	21.6114143989769
35	22.134833489372223	28.152914420473007	28.082628309587253	21.629623780567513
36	22.14443310576532	28.131939373936397	28.07107161110112	21.65255590919716
37	22.124743044933606	28.137750401336938	28.073088221323722	21.664418332405734
38	22.11613272181736	28.170906043873572	28.111372755384828	21.60158847892424
39	22.09961731279524	28.06863876202318	28.113330822675053	21.718413102506528
40	22.14068307121552	28.100448273129103	28.068849394423438	21.69001926123194
41	22.116839720389304	28.107953723593894	28.082356320853908	21.692850235162897
42	22.106040486251004	28.06436788139543	28.07722325792796	21.7523683744256
43	22.158619049055535	28.107167431892556	28.000242292707263	21.73397122634465
44	22.173028166715405	28.102487216226812	28.00428900440965	21.720195612648133
45	22.16876020209385	28.076573986948546	27.987279256892954	21.767386554064654
46	22.100752566445223	28.126015690679772	27.979948866377836	21.793282876497166
47	22.12166270011979	28.110457150014824	27.997047220280685	21.770832929584707
48	22.141782295633078	28.06184458782078	27.950565882269622	21.845807234276517
49	22.16076564607647	28.140830035728964	27.909970044102074	21.788434274092495
50	22.09140843406133	28.10369071198536	27.989438475598057	21.815462378355257
51	22.142474363507056	28.059603945596333	27.97801559929282	21.819906091603787
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.48124276767393
2	40.683779309901944
3	41.84188889675964
4	42.521305357083925
5	43.80201131749708
6	42.4473559621802
7	43.54808199040235
8	42.140196564648654
9	42.12193411897115
10	42.173040681935916
11	43.86262601616877
12	42.0387327867904
13	43.315353530981895
14	43.55172125437956
15	43.31146258233126
16	43.57881485140178
17	43.50531655624357
18	43.566756009973105
19	43.314968142737754
20	43.30933960632103
21	43.41711945876715
22	43.29376106475712
23	43.32710750433396
24	43.37886703343836
25	43.35264188111872
26	43.41003158550449
27	43.50337765435498
28	43.49220940398065
29	43.49127083448059
30	43.589207867358006
31	43.65945264532998
32	43.68472922720724
33	43.74046867879456
34	43.74751187074027
35	43.76445726993974
36	43.796989014962485
37	43.78916137733934
38	43.7177212007416
39	43.81803041530177
40	43.83070233244746
41	43.8096899555522
42	43.858408860676604
43	43.89259027540018
44	43.89322377936354
45	43.9361467561585
46	43.89403544294239
47	43.89249562970449
48	43.98758952990959
49	43.949199920168965
50	43.906870812416585
51	43.962380455110846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12463.0
1	8609.5
2	4756.0
3	6792.5
4	8829.0
5	15478.0
6	22127.0
7	28070.0
8	34013.0
9	30095.5
10	26178.0
11	31137.0
12	36096.0
13	48331.5
14	60567.0
15	86389.0
16	112211.0
17	166421.5
18	220632.0
19	279116.0
20	337600.0
21	401646.5
22	465693.0
23	550833.5
24	635974.0
25	756959.5
26	1033114.0
27	1188283.0
28	1296157.5
29	1404032.0
30	1499759.5
31	1595487.0
32	1719627.0
33	1843767.0
34	1962827.5
35	2081888.0
36	2278758.0
37	2475628.0
38	2574618.0
39	2673608.0
40	2775043.0
41	2876478.0
42	2885311.5
43	2894145.0
44	2888154.5
45	2882164.0
46	2825460.0
47	2768756.0
48	2687485.5
49	2606215.0
50	2481036.5
51	2355858.0
52	2190921.5
53	2025985.0
54	1860286.0
55	1694587.0
56	1534261.5
57	1373936.0
58	1214208.0
59	1054480.0
60	925072.5
61	795665.0
62	680666.5
63	565668.0
64	482283.0
65	398898.0
66	333884.5
67	268871.0
68	245447.5
69	222024.0
70	194020.5
71	166017.0
72	142014.5
73	118012.0
74	97810.0
75	65726.0
76	53844.0
77	50654.0
78	47464.0
79	47020.5
80	46577.0
81	41202.5
82	35828.0
83	31591.0
84	27354.0
85	24615.5
86	21877.0
87	26416.0
88	30955.0
89	21295.5
90	11636.0
91	7650.0
92	3664.0
93	2818.5
94	1973.0
95	1559.5
96	1146.0
97	875.0
98	604.0
99	389.5
100	175.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.2012523106482725E-4
2	0.0
3	0.0
4	0.0
5	0.002012215738121771
6	0.002715047072489663
7	0.027155284638145722
8	0.002476758366659453
9	0.006835756813715109
10	0.0032253218768937488
11	0.009752988242666768
12	0.027323771601864055
13	0.0048909358610806685
14	0.006361586358679236
15	0.009261969091259063
16	0.03259500660962324
17	0.0035887723272004317
18	0.001217920052021072
19	0.023761475797533644
20	0.005266421094510091
21	0.0012034783122737867
22	0.02426934364531318
23	0.001208292225522882
24	0.03140356308047219
25	0.003853537555900665
26	0.03192827962462356
27	0.031598526567060546
28	0.0030833114360454415
29	0.0381935877183209
30	0.00377892190053969
31	0.0033817740574893407
32	0.010104403909850714
33	0.01182297093977768
34	0.01564040414631013
35	0.017934233809503968
36	0.016875172894703038
37	0.02123657829838324
38	0.03171165352841428
39	0.020849058281831083
40	0.023966067110620187
41	0.025939771542749197
42	0.017332494653367076
43	0.023357107084609655
44	0.00898516907943609
45	0.02858260991650243
46	0.04000121214335612
47	0.02029545825818514
48	0.04351777577182012
49	0.020485607831524397
50	0.026353768082171384
51	0.09998257122708165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.1546241E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.54505524989597
#Duplication Level	Relative count
1	100.0
2	12.403288994468031
3	3.903876314908752
4	1.974577883940231
5	1.158891506493336
6	0.7953434348074311
7	0.5879766646761206
8	0.4593567692782193
9	0.39964038927205076
10++	9.56183926450419
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11504325	3.0968528	3.6756175	16
TGTGT	6722635	3.0274105	18.719046	4
TGTTT	6455960	2.2477937	14.683729	6
GTTTG	4525670	2.0380495	18.06716	7
GAGGG	2653335	2.035002	6.072725	11
CTTTG	4236340	1.9801956	18.876415	1
TTTGA	5247215	1.8599147	14.461786	8
GTGTT	4066685	1.8313543	17.946077	5
TTTGT	5161080	1.7969507	14.319666	2
TTGTG	3895975	1.7544783	17.925306	3
TGAGG	2901795	1.7206925	7.2098837	10
TTGAG	3376945	1.5481904	11.14625	9
ACTTT	3539150	1.3021128	5.313024	3
AACTT	3459045	1.2956101	5.62831	2
TAACT	2452245	0.91850626	5.3407955	1
>>END_MODULE