FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_6_ATCACG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_6_ATCACG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 41546241
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 6138405 2.798235 19.815664 4
TGTTT 5935775 2.055216 15.162435 6
GAGGG 2451040 1.9715738 6.617894 11
CTTTG 4022280 1.8761138 19.605537 1
GTTTG 3960795 1.8055559 18.941317 7
TTTGT 4990925 1.7280695 14.917631 2
TGAGG 2749260 1.6796931 7.794471 10
TTTGA 4734160 1.6686302 14.943491 8
GTGTT 3499645 1.5953377 18.890385 5
TTGTG 3415555 1.5570048 18.954243 3
TTGAG 3062395 1.421107 11.762914 9
Produced by FastQC (version 0.10.1)