##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_6_ATCACG_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	41546241	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59110563095227	34.0	31.0	34.0	31.0	34.0
2	32.86757545646548	34.0	31.0	34.0	31.0	34.0
3	32.962226402143095	34.0	31.0	34.0	31.0	34.0
4	36.40534694823534	37.0	37.0	37.0	35.0	37.0
5	36.31781214574864	37.0	37.0	37.0	35.0	37.0
6	36.30236162641044	37.0	37.0	37.0	35.0	37.0
7	36.2994494495904	37.0	37.0	37.0	35.0	37.0
8	36.27592233915939	37.0	37.0	37.0	35.0	37.0
9	38.022532844788536	39.0	38.0	39.0	35.0	39.0
10	38.00563004003178	39.0	38.0	39.0	35.0	39.0
11	38.00795990183564	39.0	38.0	39.0	35.0	39.0
12	37.976787310312865	39.0	38.0	39.0	35.0	39.0
13	37.97364495623082	39.0	38.0	39.0	35.0	39.0
14	39.48454802445304	41.0	39.0	41.0	37.0	41.0
15	39.48873054484039	41.0	39.0	41.0	37.0	41.0
16	39.478573308232626	41.0	39.0	41.0	37.0	41.0
17	39.45278257544407	41.0	39.0	41.0	37.0	41.0
18	39.41103121699987	41.0	39.0	41.0	36.0	41.0
19	39.42415353533428	41.0	39.0	41.0	37.0	41.0
20	39.39786449031574	41.0	39.0	41.0	36.0	41.0
21	39.37116342246222	41.0	39.0	41.0	36.0	41.0
22	39.35266656254172	41.0	39.0	41.0	36.0	41.0
23	39.33249657893238	40.0	39.0	41.0	36.0	41.0
24	39.2975745507277	41.0	39.0	41.0	36.0	41.0
25	39.250754959997465	40.0	39.0	41.0	36.0	41.0
26	39.18839105082937	40.0	39.0	41.0	36.0	41.0
27	39.1087955466296	40.0	39.0	41.0	36.0	41.0
28	39.06769093743041	40.0	39.0	41.0	36.0	41.0
29	38.9904777907585	40.0	39.0	41.0	36.0	41.0
30	38.90596836426188	40.0	38.0	41.0	35.0	41.0
31	38.85475044541334	40.0	38.0	41.0	35.0	41.0
32	38.797562070657605	40.0	38.0	41.0	35.0	41.0
33	38.72088815447828	40.0	38.0	41.0	35.0	41.0
34	38.85929574230314	40.0	38.0	41.0	35.0	41.0
35	38.87862810500714	40.0	38.0	41.0	35.0	41.0
36	38.86163633913355	40.0	38.0	41.0	35.0	41.0
37	38.838463171674185	40.0	38.0	41.0	35.0	41.0
38	38.80477475687873	40.0	38.0	41.0	35.0	41.0
39	38.72326442240587	40.0	38.0	41.0	35.0	41.0
40	38.676362152715576	40.0	38.0	41.0	35.0	41.0
41	38.63971679170686	40.0	38.0	41.0	35.0	41.0
42	38.0060612944502	40.0	38.0	41.0	33.0	41.0
43	38.25236196458784	40.0	38.0	41.0	34.0	41.0
44	38.36026166603135	40.0	38.0	41.0	34.0	41.0
45	38.367677138348085	40.0	38.0	41.0	34.0	41.0
46	38.33275734861308	40.0	38.0	41.0	34.0	41.0
47	38.27009550635399	40.0	38.0	41.0	34.0	41.0
48	38.2129523101741	40.0	38.0	41.0	34.0	41.0
49	38.04347755552662	40.0	38.0	41.0	33.0	41.0
50	38.203600561600744	40.0	38.0	41.0	34.0	41.0
51	37.98815967490296	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	12.0
11	28.0
12	53.0
13	123.0
14	176.0
15	337.0
16	571.0
17	961.0
18	1718.0
19	3234.0
20	6312.0
21	11366.0
22	19527.0
23	31385.0
24	47344.0
25	68918.0
26	96095.0
27	131638.0
28	175180.0
29	231820.0
30	302924.0
31	393331.0
32	513189.0
33	683070.0
34	964339.0
35	1414512.0
36	2155848.0
37	3651073.0
38	8318107.0
39	2.2311295E7
40	11750.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.49918552229678	29.71857585782587	28.290334510671872	23.491904109205475
2	20.89068959804435	29.12227070357336	30.854945931027316	19.13209376735497
3	19.440137208486487	27.830891483987518	31.828529060880488	20.900442246645504
4	20.288898014143104	27.814819123353207	31.265186209887787	20.6310966526159
5	23.53514725917081	27.857268723781775	28.83599024036856	19.77159377667886
6	21.89840283982377	27.61598642861544	29.930528009273523	20.555082722287263
7	23.36892524163618	28.275491879036664	28.13594375481527	20.21963912451189
8	21.110954418234854	27.782342571016233	30.352632865148983	20.754070145599933
9	20.921069610124295	27.65705325783866	30.72742008115728	20.694457050879766
10	21.20080370207259	27.898954805562315	30.280833349038726	20.619408143326375
11	23.014388156889613	27.796388577121995	28.516115363007916	20.67310790298048
12	21.163560862220965	29.704381679199326	28.358760543462886	20.77329691511682
13	22.286191110733057	28.09044708211608	28.73596160499538	20.887400202155487
14	22.000873677115578	28.162381766379298	28.84325443546144	20.99349012104368
15	22.036961177787422	28.101791447269562	28.74240776680615	21.11883960813687
16	21.998849426594333	28.05002262418879	28.72936687581435	21.221761073402526
17	22.052346177039155	28.09346159876093	28.66096190308629	21.193230321113628
18	21.93664901501186	28.05112549819641	28.702910753850137	21.309314732941594
19	21.7835615982683	28.18699530482192	28.805173974704473	21.224269122205303
20	21.698364961585813	28.19296696420742	28.828868055716523	21.279800018490242
21	21.80620383923542	28.157303087901504	28.692321406405934	21.34417166645714
22	21.76891045329468	28.201225232386246	28.707781288805407	21.32208302551367
23	21.818835547601047	28.182954024649305	28.63932744240327	21.358882985346376
24	21.768563851540744	28.184937356907934	28.584756921811532	21.461741869739793
25	21.802302162547026	28.28702360822487	28.520859444299667	21.389814784928436
26	21.848831041056158	28.22887635008905	28.490387373432895	21.4319052354219
27	21.847700778143658	28.21796610489333	28.408058757731418	21.526274359231596
28	21.8506001541752	28.229244614452604	28.433092177942164	21.487063053430035
29	21.88793445837856	28.292549980634828	28.380228189597222	21.439287371389387
30	21.910490048907192	28.20653016478675	28.35423305805211	21.528746728253946
31	21.9703527934967	28.202024090112367	28.28945693049858	21.53816618589235
32	21.980278760421864	28.250676899162784	28.246004977125395	21.523039363289957
33	21.95178150918877	28.19019934364469	28.220948322580014	21.63707082458652
34	21.972525770989908	28.240815378874963	28.197326374579163	21.58933247555597
35	21.964689948737185	28.21918377320662	28.211592170433935	21.604534107622257
36	22.01011843269478	28.205228619100385	28.133690977413927	21.650961970790902
37	22.001997725859244	28.214826944271564	28.152169049421342	21.63100628044785
38	21.988452671787293	28.2091849769103	28.205100370830245	21.597261980472165
39	22.025700088039258	28.143368163842702	28.178001864380555	21.65292988373749
40	22.001646310192058	28.189277099701993	28.17676814612422	21.63230844398173
41	22.00925630689679	28.132577307810703	28.211346347382754	21.64682003790975
42	22.109255071095845	28.217715458676395	28.023844067766746	21.64918540246101
43	22.01738243920025	28.16462826440638	28.119671065811268	21.698318230582096
44	22.06880762088681	28.13086989025072	28.09917027150543	21.70115221735704
45	22.06643195469838	28.077803717549322	28.1112868911534	21.744477436598896
46	21.985336772104127	28.149215713643024	28.116047851356758	21.749399662896096
47	22.036537034489058	28.18486948569465	28.088105833760917	21.690487646055367
48	22.02491410026649	28.09207746594149	28.090402031166168	21.79260640262585
49	22.168652995586292	28.196512411315382	27.949240943362362	21.685593649735964
50	21.974534360236238	28.136748601085042	28.09324119286129	21.795475845817432
51	22.03208266435256	28.096433879016168	28.07177509532675	21.799708361304514
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.99108963150226
2	40.02278336539932
3	40.340579455131994
4	40.91999466675901
5	43.30674103584967
6	42.45348556211104
7	43.58856436614807
8	41.865024563834794
9	41.61552666100406
10	41.820211845398966
11	43.68749605987009
12	41.936857777337785
13	43.17359131288855
14	42.99436379815926
15	43.15580078592429
16	43.22061049999686
17	43.24557649815278
18	43.24596374795345
19	43.007830720473606
20	42.97816498007606
21	43.150375505692566
22	43.09099347880835
23	43.177718532947424
24	43.23030572128054
25	43.19211694747547
26	43.28073627647805
27	43.373975137375254
28	43.33766320760523
29	43.32722182976794
30	43.43923677716114
31	43.50851897938905
32	43.50331812371182
33	43.58885233377529
34	43.561858246545874
35	43.56922405635944
36	43.66108040348568
37	43.63300400630709
38	43.585714652259455
39	43.67862997177675
40	43.633954754173786
41	43.65607634480654
42	43.75844047355685
43	43.71570066978235
44	43.76995983824385
45	43.81090939129727
46	43.73473643500022
47	43.72702468054443
48	43.817520502892336
49	43.854246645322256
50	43.77001020605367
51	43.83179102565708
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18755.0
1	12037.0
2	5319.0
3	7492.5
4	9666.0
5	12262.5
6	14859.0
7	23288.5
8	31718.0
9	28637.0
10	25556.0
11	31171.0
12	36786.0
13	50119.5
14	63453.0
15	92355.5
16	121258.0
17	179689.5
18	238121.0
19	301893.5
20	365666.0
21	432345.0
22	499024.0
23	587429.0
24	675834.0
25	803183.5
26	1091339.0
27	1252145.0
28	1352224.0
29	1452303.0
30	1542228.0
31	1632153.0
32	1750482.0
33	1868811.0
34	1986099.0
35	2103387.0
36	2292194.5
37	2481002.0
38	2572962.0
39	2664922.0
40	2755847.5
41	2846773.0
42	2853127.5
43	2859482.0
44	2852189.5
45	2844897.0
46	2787564.0
47	2730231.0
48	2651660.5
49	2573090.0
50	2446938.0
51	2320786.0
52	2164454.5
53	2008123.0
54	1841720.0
55	1675317.0
56	1515957.5
57	1356598.0
58	1196256.5
59	1035915.0
60	909318.5
61	782722.0
62	667999.0
63	553276.0
64	472201.0
65	391126.0
66	326002.5
67	260879.0
68	238712.0
69	216545.0
70	188990.0
71	161435.0
72	137159.5
73	112884.0
74	93464.0
75	61577.0
76	49110.0
77	46946.5
78	44783.0
79	44331.5
80	43880.0
81	38795.0
82	33710.0
83	28626.0
84	23542.0
85	21124.0
86	18706.0
87	23492.5
88	28279.0
89	17880.5
90	7482.0
91	4204.0
92	926.0
93	622.0
94	318.0
95	199.0
96	80.0
97	51.5
98	23.0
99	15.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026558359395257924
2	3.3697392743666025E-4
3	0.005909078513264293
4	0.04337817228759637
5	0.0
6	2.4069566245475735E-6
7	0.0
8	0.0
9	0.0
10	0.0
11	0.006161808958841788
12	0.0
13	7.22086987364272E-6
14	0.0
15	0.0
16	0.0
17	0.008501370797902029
18	0.004532299324023081
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	4.4528697554130104E-4
28	0.0
29	0.0
30	0.0
31	1.6126609384468742E-4
32	4.1158958279763504E-4
33	3.5141566718394573E-4
34	2.5754435882659036E-4
35	8.111443824725322E-4
36	6.763548114978681E-4
37	0.0
38	1.6848696371833016E-5
39	4.813913249095147E-6
40	0.0
41	0.0017570783359197285
42	3.417878406857554E-4
43	1.3960348422375926E-4
44	0.0
45	0.0
46	0.0
47	0.0066167237608812796
48	0.01151728744846014
49	0.0
50	0.014896654549324932
51	0.037069538974657174
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.1546241E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.696609795558167
#Duplication Level	Relative count
1	100.0
2	12.928598904378507
3	4.064808796805985
4	2.0479898131076655
5	1.2302529479646112
6	0.8416123940523405
7	0.6525977901871576
8	0.48878513350399916
9	0.3886405539122707
10++	9.998540939899987
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	6138405	2.798235	19.815664	4
TGTTT	5935775	2.055216	15.162435	6
GAGGG	2451040	1.9715738	6.617894	11
CTTTG	4022280	1.8761138	19.605537	1
GTTTG	3960795	1.8055559	18.941317	7
TTTGT	4990925	1.7280695	14.917631	2
TGAGG	2749260	1.6796931	7.794471	10
TTTGA	4734160	1.6686302	14.943491	8
GTGTT	3499645	1.5953377	18.890385	5
TTGTG	3415555	1.5570048	18.954243	3
TTGAG	3062395	1.421107	11.762914	9
>>END_MODULE