FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_3_ACAGTG_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_3_ACAGTG_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33598590
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 10518835 3.3170695 3.9709873 16
TGTGT 4984305 2.7565114 18.05407 4
TGTTT 5307585 2.2165015 13.962518 6
GTTTG 3745960 2.0716593 17.515566 7
GAGGG 2061460 2.0405486 6.3161316 11
CTTTG 3363955 1.9308851 18.320063 1
TTTGA 4310920 1.8373402 13.78506 8
GTGTT 3238565 1.7910504 17.407518 5
TGAGG 2323820 1.7369621 7.2097397 10
TTTGT 4158190 1.7365024 13.595482 2
TTGTG 3070515 1.6981122 17.39981 3
TTGAG 2744635 1.5491316 10.927537 9
AACTT 3018210 1.3626008 5.4395547 2
ACTTT 2904060 1.2846246 5.084946 3
TAACT 2022505 0.9130799 5.0634575 1
Produced by FastQC (version 0.10.1)