##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_3_ACAGTG_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	33598590	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34429191820252	34.0	31.0	34.0	31.0	34.0
2	32.4988436717136	34.0	31.0	34.0	31.0	34.0
3	32.54813306153621	34.0	31.0	34.0	31.0	34.0
4	35.99510024081368	37.0	37.0	37.0	35.0	37.0
5	35.99812072470898	37.0	37.0	37.0	35.0	37.0
6	36.00948423728496	37.0	37.0	37.0	35.0	37.0
7	36.000419958099435	37.0	37.0	37.0	35.0	37.0
8	36.0175791305528	37.0	37.0	37.0	35.0	37.0
9	37.76495623774688	39.0	38.0	39.0	35.0	39.0
10	37.750572032933526	39.0	38.0	39.0	35.0	39.0
11	37.71375117229622	39.0	38.0	39.0	35.0	39.0
12	37.679658729726455	39.0	38.0	39.0	35.0	39.0
13	37.674862665367804	39.0	38.0	39.0	35.0	39.0
14	39.18323885020175	41.0	39.0	41.0	36.0	41.0
15	39.15170571145992	41.0	39.0	41.0	36.0	41.0
16	39.10339219592251	41.0	39.0	41.0	36.0	41.0
17	39.093476690539696	41.0	39.0	41.0	36.0	41.0
18	39.064124982625756	41.0	39.0	41.0	36.0	41.0
19	39.07581526486677	41.0	39.0	41.0	36.0	41.0
20	39.0416470155444	41.0	39.0	41.0	36.0	41.0
21	39.010450795703036	41.0	39.0	41.0	36.0	41.0
22	38.96191158617073	41.0	39.0	41.0	36.0	41.0
23	38.92684969815698	41.0	39.0	41.0	36.0	41.0
24	38.86119866934892	41.0	39.0	41.0	36.0	41.0
25	38.825516011237376	41.0	39.0	41.0	35.0	41.0
26	38.7855380835922	41.0	39.0	41.0	35.0	41.0
27	38.731431973782236	40.0	39.0	41.0	35.0	41.0
28	38.68209606415031	40.0	39.0	41.0	35.0	41.0
29	38.57661181615062	40.0	39.0	41.0	35.0	41.0
30	38.5628221600966	40.0	39.0	41.0	35.0	41.0
31	38.51919473406473	40.0	38.0	41.0	34.0	41.0
32	38.46662916509294	40.0	38.0	41.0	34.0	41.0
33	38.415095693003785	40.0	38.0	41.0	34.0	41.0
34	38.344717947985316	40.0	38.0	41.0	34.0	41.0
35	38.30477064662535	40.0	38.0	41.0	34.0	41.0
36	38.25465994257497	40.0	38.0	41.0	34.0	41.0
37	38.19443765348486	40.0	38.0	41.0	34.0	41.0
38	38.12557241241374	40.0	38.0	41.0	33.0	41.0
39	38.075806841894256	40.0	38.0	41.0	33.0	41.0
40	38.000141404743474	40.0	38.0	41.0	33.0	41.0
41	38.18765638677099	40.0	38.0	41.0	34.0	41.0
42	38.22732284301216	40.0	38.0	41.0	34.0	41.0
43	38.21118415981147	40.0	38.0	41.0	34.0	41.0
44	38.167400804617095	40.0	38.0	41.0	33.0	41.0
45	38.13349325075844	40.0	38.0	41.0	33.0	41.0
46	38.0706632629524	40.0	38.0	41.0	33.0	41.0
47	38.02365399857553	40.0	38.0	41.0	33.0	41.0
48	37.959573422575176	40.0	38.0	41.0	33.0	41.0
49	37.89934657972254	40.0	38.0	41.0	33.0	41.0
50	37.801182132940696	40.0	38.0	41.0	33.0	41.0
51	36.32784991870194	38.0	36.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	15.0
5	89.0
6	597.0
7	2379.0
8	7715.0
9	12907.0
10	16971.0
11	21467.0
12	28117.0
13	36987.0
14	45280.0
15	51105.0
16	52395.0
17	53778.0
18	54863.0
19	55363.0
20	58004.0
21	61789.0
22	67441.0
23	74175.0
24	84503.0
25	95816.0
26	109783.0
27	128266.0
28	151928.0
29	183074.0
30	224896.0
31	282263.0
32	366057.0
33	489603.0
34	694541.0
35	1023625.0
36	1578781.0
37	2678006.0
38	6414020.0
39	1.8387995E7
40	3995.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.949938611960622	29.43175361445335	28.829290651903772	22.789017121682257
2	22.160067094797416	29.913155716338398	29.924698126391235	18.00207906247295
3	21.288872864395465	28.361454473979634	30.2738277580061	20.075844903618805
4	21.066219606533785	27.41629282268061	30.687641872189214	20.82984569859639
5	23.570933142515564	27.695392981387023	29.16209472642487	19.57157914967254
6	21.664337699885618	27.437324006751474	30.59435827515381	20.303980018209096
7	23.164651496150203	28.072435337679774	28.930040952577063	19.83287221359296
8	21.308393968859722	27.669893318975458	30.711432040142515	20.310280672022294
9	21.364965458747463	27.494023861336974	30.84915920335772	20.29185147655785
10	21.491659963778208	28.300907663961222	30.000944119102353	20.206488253158213
11	23.052244927287624	27.674090052363127	28.943366877322312	20.33029814302694
12	21.162211208334636	29.448718583436083	28.926669520844882	20.462400687384395
13	22.28175854236418	27.932260842111816	29.2380627716768	20.547917843847205
14	22.349246714653994	28.085479107136802	28.902267108339203	20.663007069870005
15	21.983863034472805	28.446310694999745	28.76272499369359	20.807101276833862
16	22.091196736023157	28.08405584867963	28.903936058080465	20.92081135721675
17	22.049537442908687	28.125836317050133	28.94174391975273	20.88288232028844
18	21.95259944221717	28.066858340584922	28.917557069193307	21.0629851480046
19	21.783683334064737	28.196940932534876	29.05366810202306	20.965707631377327
20	21.711400127152242	28.225601175324844	29.021689696144236	21.04130900137867
21	21.754416491114632	28.207024466404462	28.960832168128974	21.077726874351928
22	21.68887118580542	28.286808409421116	28.98234906322755	21.041971341545917
23	21.71509995406932	28.316949724286445	28.896371000111586	21.071579321532642
24	21.669725325961096	28.334868024868676	28.840130658489905	21.155275990680323
25	21.687945401056567	28.415298203592243	28.769710548711952	21.12704584663924
26	21.719575492495878	28.405383505007443	28.765487987913335	21.109553014583344
27	21.718803617811627	28.350646199892655	28.677300687898377	21.253249494397345
28	21.721675073259593	28.336795974693928	28.713233018980443	21.228295933066033
29	21.73800857021629	28.44012994810763	28.664520607064038	21.15734087461204
30	21.752542693581194	28.388117774997983	28.5700885366907	21.289250994730118
31	21.826964512903686	28.371725179194947	28.534843076688578	21.266467231212793
32	21.82206114154985	28.41406433032075	28.480945632057626	21.282928896071773
33	21.811723732552657	28.3831696978709	28.439910352147802	21.36519621742864
34	21.8633639164236	28.412904559696013	28.376192822548713	21.34753870133168
35	21.803943105221407	28.401451391338384	28.420159337527366	21.374446165912843
36	21.82926345020653	28.403717745261154	28.374525889071663	21.392492915460657
37	21.855196622628775	28.38907638005696	28.388430434740545	21.36729656257372
38	21.809044759257887	28.412848262729106	28.42117422027452	21.356932757738488
39	21.800005012924952	28.318060681516076	28.444550709456884	21.437383596102087
40	21.850160054670358	28.340064903187113	28.398556494808673	21.411218547333853
41	21.816513177028618	28.360163879721117	28.38085573363205	21.442467209618215
42	21.801661898526447	28.33126384646278	28.34356139424959	21.523512860761187
43	21.82702756776423	28.40515806641582	28.313952276691108	21.453862089128844
44	21.86419292551975	28.408601521921845	28.274881788576668	21.452323763981738
45	21.853376951080865	28.358525523078175	28.300123464262693	21.487974061578267
46	21.826232483228477	28.403302169282668	28.277020968872	21.493444378616854
47	21.83313771411997	28.39752047213473	28.276689309508736	21.492652504236563
48	21.838359849448416	28.359743431206475	28.255259897912577	21.546636821432536
49	21.839924318016372	28.448239245839975	28.19044354522695	21.5213928909167
50	21.818759967097183	28.41184394113099	28.26036312973316	21.509032962038667
51	21.851644212845937	28.324840112115528	28.2916323304025	21.53188334463604
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.73895573364288
2	40.16214615727037
3	41.364717768014266
4	41.89606530513018
5	43.14251229218811
6	41.96831771809472
7	42.99752370974317
8	41.61867464088202
9	41.65681693530531
10	41.698148216936424
11	43.38254307031456
12	41.624611895719035
13	42.82967638621138
14	43.012253784524
15	42.790964311306666
16	43.012008093239906
17	42.932419763197124
18	43.01558459022177
19	42.74939096544206
20	42.75270912853092
21	42.832143365466564
22	42.730842527351335
23	42.78667927560196
24	42.82500131664142
25	42.81499124769581
26	42.829128507079226
27	42.972053112208975
28	42.949971006325626
29	42.89534944482833
30	43.041793688311316
31	43.09343174411649
32	43.104990037621626
33	43.1769199499813
34	43.21090261775528
35	43.17838927113425
36	43.22175636566718
37	43.222493185202495
38	43.16597751699637
39	43.237388609027036
40	43.261378602004214
41	43.25898038664683
42	43.325174759287634
43	43.28088965689307
44	43.31651668950149
45	43.34135101265913
46	43.31967686184533
47	43.32579021835654
48	43.38499667088095
49	43.36131720893307
50	43.32779292913585
51	43.383527557481976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13107.0
1	9027.5
2	4948.0
3	7310.5
4	9673.0
5	16829.0
6	23985.0
7	29177.0
8	34369.0
9	28729.0
10	23089.0
11	26517.0
12	29945.0
13	39507.0
14	49069.0
15	71449.0
16	93829.0
17	148503.0
18	203177.0
19	253057.5
20	302938.0
21	352239.5
22	401541.0
23	471738.0
24	541935.0
25	646586.5
26	892272.5
27	1033307.0
28	1118281.5
29	1203256.0
30	1266527.5
31	1329799.0
32	1427772.5
33	1525746.0
34	1617538.0
35	1709330.0
36	1889200.0
37	2069070.0
38	2139431.0
39	2209792.0
40	2284603.0
41	2359414.0
42	2350639.5
43	2341865.0
44	2329912.0
45	2317959.0
46	2256996.0
47	2196033.0
48	2122219.5
49	2048406.0
50	1948162.0
51	1847918.0
52	1722458.0
53	1596998.0
54	1470255.0
55	1343512.0
56	1216536.5
57	1089561.0
58	968160.0
59	846759.0
60	738447.0
61	630135.0
62	535168.0
63	440201.0
64	370349.5
65	300498.0
66	249877.0
67	199256.0
68	169178.0
69	139100.0
70	119121.5
71	99143.0
72	82686.0
73	66229.0
74	55544.5
75	37779.5
76	30699.0
77	27419.5
78	24140.0
79	22536.0
80	20932.0
81	18459.5
82	15987.0
83	13740.0
84	11493.0
85	9979.5
86	8466.0
87	8686.5
88	8907.0
89	6360.5
90	3814.0
91	2683.0
92	1552.0
93	1205.5
94	859.0
95	651.5
96	444.0
97	339.0
98	234.0
99	153.5
100	73.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002500104915116974
2	0.0022441417928549976
3	7.14315690033421E-5
4	3.779920526426853E-4
5	0.0042829178248253875
6	0.0
7	0.020265731389323183
8	8.928946125417762E-4
9	0.003651938965295865
10	0.0033751416354079146
11	0.00893787507154318
12	0.024015888761998644
13	0.014595850599682903
14	0.007015175339203223
15	0.005402012405877747
16	0.04907646422067116
17	0.0078247331212411
18	6.220499134041041E-4
19	0.030310795780418166
20	0.0031846574513990024
21	0.0022739049466063903
22	0.022188431121663143
23	0.006872312201196538
24	0.08430710931619452
25	0.03573364239392189
26	0.02729876462077724
27	0.06496701200853965
28	0.009914106514588857
29	0.09695644966053635
30	0.05170157438154399
31	0.026117167416846956
32	0.03192693502911878
33	0.021295536509121367
34	0.03750455004212975
35	0.009643261815451184
36	0.010726640612001874
37	0.013140432381239808
38	0.050201511432473804
39	0.016155439856255872
40	0.03740037900399987
41	0.01684594502328818
42	0.019426410453533913
43	0.03217396920525534
44	0.011006414257264964
45	0.03194479292136962
46	0.03229599813563605
47	0.03341806903206355
48	0.056954771018664765
49	0.006604443817434006
50	0.09120025572501704
51	0.12858277683676608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.359859E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	33.19155091284675
#Duplication Level	Relative count
1	100.0
2	13.943072103707012
3	4.914039823817159
4	2.4532986921604047
5	1.5290830237955089
6	1.113659582817437
7	0.7888985866130811
8	0.6183990636057943
9	0.5006732024817153
10++	9.455957672816828
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	10518835	3.3170695	3.9709873	16
TGTGT	4984305	2.7565114	18.05407	4
TGTTT	5307585	2.2165015	13.962518	6
GTTTG	3745960	2.0716593	17.515566	7
GAGGG	2061460	2.0405486	6.3161316	11
CTTTG	3363955	1.9308851	18.320063	1
TTTGA	4310920	1.8373402	13.78506	8
GTGTT	3238565	1.7910504	17.407518	5
TGAGG	2323820	1.7369621	7.2097397	10
TTTGT	4158190	1.7365024	13.595482	2
TTGTG	3070515	1.6981122	17.39981	3
TTGAG	2744635	1.5491316	10.927537	9
AACTT	3018210	1.3626008	5.4395547	2
ACTTT	2904060	1.2846246	5.084946	3
TAACT	2022505	0.9130799	5.0634575	1
>>END_MODULE