FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_3_ACAGTG_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_3_ACAGTG_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33598590
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 10223860 3.2293143 3.8103368 16
TGTGT 4483125 2.5581229 18.932259 4
TGTTT 4817685 2.0452971 14.360369 6
GAGGG 1886175 1.9842774 6.632032 11
GTTTG 3250735 1.854907 18.286879 7
CTTTG 3157360 1.8435953 18.843388 1
TGAGG 2166665 1.6958585 7.6439066 10
TTTGT 3942220 1.6736277 14.065202 2
TTTGA 3843400 1.6652075 14.172022 8
GTGTT 2756595 1.572945 18.190052 5
TTGTG 2657740 1.5165372 18.207548 3
TTGAG 2448045 1.4255902 11.382845 9