##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_3_ACAGTG_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	33598590	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51743796391456	34.0	31.0	34.0	31.0	34.0
2	32.85254899089515	34.0	31.0	34.0	31.0	34.0
3	32.98185450639446	34.0	31.0	34.0	31.0	34.0
4	36.4528016800705	37.0	37.0	37.0	35.0	37.0
5	36.37666735419552	37.0	37.0	37.0	35.0	37.0
6	36.360057936955094	37.0	37.0	37.0	35.0	37.0
7	36.34701102040294	37.0	37.0	37.0	35.0	37.0
8	36.34616780049401	37.0	37.0	37.0	35.0	37.0
9	38.11942926771629	39.0	38.0	39.0	37.0	39.0
10	38.11690505464664	39.0	38.0	39.0	37.0	39.0
11	38.096156386324544	39.0	38.0	39.0	37.0	39.0
12	38.088669970972	39.0	38.0	39.0	37.0	39.0
13	38.07378547730723	39.0	38.0	39.0	37.0	39.0
14	39.618169066023306	41.0	40.0	41.0	37.0	41.0
15	39.61648896575719	41.0	40.0	41.0	37.0	41.0
16	39.60601135345263	41.0	40.0	41.0	37.0	41.0
17	39.56082517153249	41.0	40.0	41.0	37.0	41.0
18	39.544137328381936	41.0	40.0	41.0	37.0	41.0
19	39.553639542611755	41.0	40.0	41.0	37.0	41.0
20	39.532222691487945	41.0	40.0	41.0	37.0	41.0
21	39.51907240750281	41.0	39.0	41.0	37.0	41.0
22	39.48956509782107	41.0	39.0	41.0	37.0	41.0
23	39.46031619184019	41.0	39.0	41.0	37.0	41.0
24	39.43944817327156	41.0	39.0	41.0	37.0	41.0
25	39.395542193883735	41.0	39.0	41.0	36.0	41.0
26	39.332638095824855	41.0	39.0	41.0	36.0	41.0
27	39.27323206122638	40.0	39.0	41.0	36.0	41.0
28	39.207504392297416	40.0	39.0	41.0	36.0	41.0
29	39.149536721630284	40.0	39.0	41.0	36.0	41.0
30	39.095405848876396	40.0	39.0	41.0	36.0	41.0
31	39.03393550146003	40.0	39.0	41.0	36.0	41.0
32	32.47900352365977	40.0	35.0	41.0	2.0	41.0
33	38.904705495081785	40.0	38.0	41.0	35.0	41.0
34	39.01953102793897	40.0	39.0	41.0	36.0	41.0
35	39.04900800896704	40.0	39.0	41.0	36.0	41.0
36	39.04217331739219	40.0	39.0	41.0	36.0	41.0
37	39.007526833715346	40.0	39.0	41.0	35.0	41.0
38	38.96248458640675	40.0	39.0	41.0	35.0	41.0
39	38.83665112137146	40.0	39.0	41.0	35.0	41.0
40	38.732225518987555	40.0	38.0	41.0	35.0	41.0
41	38.76012234441981	40.0	38.0	41.0	35.0	41.0
42	38.76258462036651	40.0	38.0	41.0	35.0	41.0
43	38.71971972633376	40.0	38.0	41.0	35.0	41.0
44	38.647506100702444	40.0	38.0	41.0	35.0	41.0
45	38.63208720961207	40.0	38.0	41.0	35.0	41.0
46	38.55531586295734	40.0	38.0	41.0	35.0	41.0
47	38.49801485717109	40.0	38.0	41.0	35.0	41.0
48	38.43804487033533	40.0	38.0	41.0	34.0	41.0
49	38.40008375351466	40.0	38.0	41.0	34.0	41.0
50	38.22441748299557	40.0	38.0	41.0	34.0	41.0
51	38.07107390518471	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	8.0
11	28.0
12	49.0
13	131.0
14	177.0
15	304.0
16	468.0
17	771.0
18	1355.0
19	2435.0
20	4519.0
21	8129.0
22	13890.0
23	21989.0
24	33643.0
25	48051.0
26	67549.0
27	92232.0
28	125298.0
29	165571.0
30	218428.0
31	286461.0
32	375404.0
33	508861.0
34	724284.0
35	1081768.0
36	1690445.0
37	3107934.0
38	8417003.0
39	1.6593618E7
40	7784.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.997850687246594	29.903919801542543	28.692589926035726	23.405639585175138
2	20.937042298501215	29.28360088920398	31.01885227921767	18.760504533077132
3	19.34247390492013	27.958184770694018	31.990683954615847	20.708657369770012
4	20.0094550524912	27.96186039396766	31.564012697125026	20.464671856416107
5	23.240924693566008	27.99019244557584	29.24046515047209	19.528417710386062
6	21.793897799930765	27.797276743113798	30.132118565469174	20.276706891486267
7	23.1782286101887	28.56460643140084	28.318545510391953	19.938619448018503
8	20.93950371131646	27.925273054613303	30.607933249579816	20.52728998449042
9	20.729866937868525	27.786049950310414	30.99696743226427	20.48711567955679
10	21.05782117642437	28.08315468000294	30.456653091692242	20.40237105188045
11	22.783248456794485	28.018788032492846	28.739220955373213	20.45874255533945
12	21.005807088928435	29.81307251286438	28.60845053319202	20.57266986501517
13	22.102335246806486	28.26304913390711	28.991070756243044	20.64354486304336
14	21.78538146987716	28.34129051248877	29.10742087688799	20.765907140746084
15	21.78825063789879	28.28068677882018	29.011529948131752	20.919532635149274
16	21.746269709532452	28.240536284409558	29.028253864224656	20.984940141833334
17	21.825422575886726	28.286961303035042	28.961431269645228	20.926184851433007
18	21.69102626456611	28.216551347396535	28.994911403828883	21.097510984208473
19	21.536875389897663	28.363792972079754	29.110217818858903	20.98911381916368
20	21.46772167087527	28.349805881102704	29.116135100359326	21.066337347662703
21	21.5383949147866	28.319271731343488	29.01773258937354	21.124600764496368
22	21.54516900858042	28.35106175586535	29.01293179267344	21.09083744288079
23	21.58675111068649	28.387453163957176	28.915302695738127	21.11049302961821
24	21.52857902667939	28.370741153125774	28.875399235503636	21.225280584691202
25	21.56318166923076	28.455646501832373	28.80895299475365	21.17221883418322
26	21.637229419448854	28.442253082644243	28.786830042570237	21.133687455336666
27	21.61529695144945	28.40079300946855	28.69822811016772	21.28568192891428
28	21.63441382510397	28.38984612151879	28.727163848244825	21.24857620513242
29	21.62808740659171	28.457070781554922	28.72046395772661	21.19437785412676
30	21.62843143119994	28.395060626056036	28.663696899185354	21.312811043558675
31	21.71944120274095	28.394221305120244	28.60550398097063	21.280833511168176
32	21.715946452691817	28.476171788484685	28.532277690331938	21.275604068491564
33	21.69982728441878	28.412284563131962	28.517071698544495	21.370816453904762
34	21.740469539015052	28.454238131606406	28.46058671244709	21.344705616931453
35	21.68760750491451	28.44712782108135	28.48713560238944	21.3781290716147
36	21.727957080073	28.44334487370681	28.43463616869839	21.394061877521796
37	21.757130284336338	28.450128413126862	28.434544425822633	21.35819687671417
38	21.743986875639724	28.468135121146453	28.460959224776992	21.32691877843683
39	21.773732171498864	28.411120823820283	28.42625241118749	21.388894593493358
40	21.80101903085814	28.480995779882427	28.368351171879535	21.349634017379895
41	21.72933753165578	28.391423484846996	28.455742823156893	21.42349616034033
42	21.744100637622658	28.3394558911237	28.406407074026152	21.510036397227495
43	21.760038144457848	28.414171547079803	28.36890476653931	21.45688554192304
44	21.806519261671397	28.417296678223696	28.310179087872438	21.466004972232465
45	21.799248420935708	28.341476957807863	28.394158567836442	21.465116053419987
46	21.757891250323986	28.378388433948615	28.394636867890082	21.469083447837317
47	21.779925099992397	28.395295646534546	28.372537965735418	21.45224128773764
48	21.77136148926818	28.36777165995913	28.356901975687148	21.50396487508554
49	21.769390600164897	28.432012838198677	28.3016617149054	21.496934846731026
50	21.757163480408607	28.540592547101753	28.244252016860344	21.457991955629296
51	21.799609164697547	28.33319677833489	28.329109066526915	21.53808499044065
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.40349027242173
2	39.69754683157835
3	40.05113127469014
4	40.4741269089073
5	42.76934240395207
6	42.07060469141703
7	43.116848058207204
8	41.46679369580688
9	41.216982617425316
10	41.46019222830482
11	43.24199101213394
12	41.57847695394361
13	42.74588010984985
14	42.551288610623246
15	42.707783273048065
16	42.73120985136579
17	42.75160742731973
18	42.788537248774574
19	42.52598920906134
20	42.534059018537974
21	42.66299567928297
22	42.636006451461206
23	42.6972441403047
24	42.7538596113706
25	42.735400503413985
26	42.77091687478552
27	42.90097888036372
28	42.88299003023639
29	42.82246526071847
30	42.94124247475861
31	43.00027471390913
32	42.99155052118338
33	43.07064373832355
34	43.0851751559465
35	43.06573657652921
36	43.1220189575948
37	43.11532716105051
38	43.070905654076554
39	43.162626764992226
40	43.150653048238034
41	43.152833691996115
42	43.25413703485015
43	43.21692368638089
44	43.272524233903866
45	43.264364474355695
46	43.2269746981613
47	43.23216638773004
48	43.275326364353724
49	43.26632544689592
50	43.215155436037904
51	43.337694155138195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21191.0
1	13324.0
2	5457.0
3	8477.0
4	11497.0
5	13362.5
6	15228.0
7	23756.0
8	32284.0
9	27298.0
10	22312.0
11	25893.5
12	29475.0
13	40211.0
14	50947.0
15	77276.0
16	103605.0
17	162071.0
18	220537.0
19	273170.0
20	325803.0
21	377854.0
22	429905.0
23	503537.0
24	577169.0
25	688882.0
26	943055.0
27	1085515.0
28	1159719.5
29	1233924.0
30	1297145.5
31	1360367.0
32	1454897.0
33	1549427.0
34	1644710.5
35	1739994.0
36	1914424.0
37	2088854.0
38	2152105.0
39	2215356.0
40	2282211.5
41	2349067.0
42	2339339.0
43	2329611.0
44	2315802.0
45	2301993.0
46	2237785.0
47	2173577.0
48	2098369.0
49	2023161.0
50	1920157.0
51	1817153.0
52	1693292.5
53	1569432.0
54	1442318.5
55	1315205.0
56	1186913.5
57	1058622.0
58	938562.5
59	818503.0
60	712147.5
61	605792.0
62	512174.5
63	418557.0
64	350613.5
65	282670.0
66	234474.5
67	186279.0
68	158271.0
69	130263.0
70	111034.0
71	91805.0
72	76192.5
73	60580.0
74	50351.5
75	33372.5
76	26622.0
77	24203.0
78	21784.0
79	20259.0
80	18734.0
81	16293.5
82	13853.0
83	11640.0
84	9427.0
85	8185.0
86	6943.0
87	6857.5
88	6772.0
89	4378.0
90	1984.0
91	1209.5
92	435.0
93	291.5
94	148.0
95	93.5
96	39.0
97	25.5
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.024756991290408318
2	0.0
3	0.0057472649893939
4	0.030355440511045256
5	0.0
6	0.001520897156696159
7	0.0
8	0.0
9	0.0
10	0.0
11	5.387130829002051E-4
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.011649298378295042
18	3.839446833929638E-4
19	8.928946125417764E-5
20	0.0010923077426761062
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0015744708334486657
30	0.0
31	0.0
32	17.515401687987502
33	0.0
34	0.001577447148823805
35	3.7501573726754603E-4
36	7.14315690033421E-5
37	0.0
38	0.0
39	0.0
40	0.0
41	0.02957266956738363
42	0.0029048838061359124
43	0.0
44	0.0
45	0.0015714945180735262
46	0.004482330954959717
47	0.01647688191677091
48	0.0016607839793277039
49	3.1846574513990023E-4
50	0.01831922113398211
51	0.10292098567231542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.359859E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.69255852654778
#Duplication Level	Relative count
1	100.0
2	13.55915214369721
3	4.605474179407676
4	2.3281300117609325
5	1.4380412701806906
6	0.9482251683951674
7	0.7270394525820593
8	0.5553031113011868
9	0.4604137709825724
10++	9.025179086923982
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	10223860	3.2293143	3.8103368	16
TGTGT	4483125	2.5581229	18.932259	4
TGTTT	4817685	2.0452971	14.360369	6
GAGGG	1886175	1.9842774	6.632032	11
GTTTG	3250735	1.854907	18.286879	7
CTTTG	3157360	1.8435953	18.843388	1
TGAGG	2166665	1.6958585	7.6439066	10
TTTGT	3942220	1.6736277	14.065202	2
TTTGA	3843400	1.6652075	14.172022	8
GTGTT	2756595	1.572945	18.190052	5
TTGTG	2657740	1.5165372	18.207548	3
TTGAG	2448045	1.4255902	11.382845	9
>>END_MODULE