FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_2_ATCACG_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_2_ATCACG_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 40336508
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11391775 3.273765 3.8806098 16
TGTGT 5798815 2.7050114 18.050505 4
TGTTT 6085240 2.2280285 14.454892 6
GTTTG 4350960 2.0296211 17.499365 7
GAGGG 2585460 1.9932886 5.8576584 11
CTTTG 4043395 1.9521725 18.324532 1
TTTGA 5043925 1.8803573 14.281598 8
GTGTT 3826375 1.7849144 17.412888 5
TTTGT 4808075 1.7604115 14.1223955 2
TTGTG 3611080 1.6844844 17.397736 3
TGAGG 2761770 1.6712177 6.8447704 10
TTGAG 3202600 1.5211127 10.701814 9
AACTT 3502500 1.376008 5.6872883 2
ACTTT 3435900 1.3257283 5.319982 3
TAACT 2395070 0.94093806 5.351978 1
Produced by FastQC (version 0.10.1)