##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_2_ATCACG_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	40336508	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.179032676799885	33.0	31.0	34.0	31.0	34.0
2	32.35067321643212	34.0	31.0	34.0	31.0	34.0
3	32.399738966000726	34.0	31.0	34.0	31.0	34.0
4	35.889861090603084	37.0	35.0	37.0	35.0	37.0
5	35.877519913226	37.0	37.0	37.0	35.0	37.0
6	35.90976576356089	37.0	36.0	37.0	35.0	37.0
7	35.90962264259464	37.0	37.0	37.0	35.0	37.0
8	35.91732722624378	37.0	37.0	37.0	35.0	37.0
9	37.60630828528836	39.0	38.0	39.0	35.0	39.0
10	37.59189375044563	39.0	38.0	39.0	35.0	39.0
11	37.605503629615136	39.0	38.0	39.0	35.0	39.0
12	37.577035597627834	39.0	38.0	39.0	35.0	39.0
13	37.51317035178157	39.0	38.0	39.0	35.0	39.0
14	38.97371235011221	41.0	39.0	41.0	36.0	41.0
15	38.93998491391471	41.0	39.0	41.0	36.0	41.0
16	38.89536964875591	41.0	39.0	41.0	36.0	41.0
17	38.8483329543549	41.0	39.0	41.0	36.0	41.0
18	38.85435660419588	41.0	39.0	41.0	36.0	41.0
19	38.85872703705537	41.0	39.0	41.0	35.0	41.0
20	38.845606887933876	41.0	39.0	41.0	35.0	41.0
21	38.815790251352446	40.0	39.0	41.0	35.0	41.0
22	38.759281418213	40.0	39.0	41.0	35.0	41.0
23	38.67759130264821	40.0	39.0	41.0	35.0	41.0
24	38.623214409140225	40.0	39.0	41.0	34.0	41.0
25	38.60666151368383	40.0	39.0	41.0	34.0	41.0
26	38.558827774580784	40.0	39.0	41.0	34.0	41.0
27	38.495526186847904	40.0	38.0	41.0	34.0	41.0
28	38.45056584967643	40.0	38.0	41.0	34.0	41.0
29	38.39162014222946	40.0	38.0	41.0	34.0	41.0
30	38.35232278907237	40.0	38.0	41.0	34.0	41.0
31	38.29586277027253	40.0	38.0	41.0	34.0	41.0
32	38.22843965570842	40.0	38.0	41.0	34.0	41.0
33	38.16893889277674	40.0	38.0	41.0	33.0	41.0
34	38.10386888225426	40.0	38.0	41.0	33.0	41.0
35	38.03031509321531	40.0	38.0	41.0	33.0	41.0
36	37.97587622111463	40.0	38.0	41.0	33.0	41.0
37	37.887939382358034	40.0	38.0	41.0	33.0	41.0
38	37.84524694105895	40.0	38.0	41.0	33.0	41.0
39	37.76801918500233	40.0	38.0	41.0	33.0	41.0
40	37.69768243200428	40.0	38.0	41.0	32.0	41.0
41	37.873598973912166	40.0	38.0	41.0	33.0	41.0
42	37.91334423396294	40.0	38.0	41.0	33.0	41.0
43	37.91966186066479	40.0	38.0	41.0	33.0	41.0
44	37.875110036793465	40.0	38.0	41.0	33.0	41.0
45	37.83425605409373	40.0	38.0	41.0	33.0	41.0
46	37.7629065956825	40.0	38.0	41.0	33.0	41.0
47	37.72443388009691	40.0	38.0	41.0	33.0	41.0
48	37.65856722153539	40.0	37.0	41.0	33.0	41.0
49	37.58846117765078	40.0	37.0	41.0	32.0	41.0
50	37.48302708801664	40.0	37.0	41.0	32.0	41.0
51	35.97133733044021	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	159.0
3	162.0
4	141.0
5	254.0
6	633.0
7	2791.0
8	8262.0
9	13270.0
10	17719.0
11	24164.0
12	34130.0
13	46467.0
14	57158.0
15	63873.0
16	67919.0
17	69574.0
18	70552.0
19	72391.0
20	76330.0
21	81193.0
22	90294.0
23	100397.0
24	113617.0
25	129608.0
26	150719.0
27	177202.0
28	211244.0
29	256147.0
30	315573.0
31	399436.0
32	516832.0
33	695954.0
34	988405.0
35	1450648.0
36	2213149.0
37	3681102.0
38	8222153.0
39	1.9913529E7
40	3357.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.556416298920933	28.84726058239857	28.315159074237982	23.281164044442516
2	22.65421208149802	29.471368139924543	29.455299706895516	18.41912007168192
3	21.61855094298607	27.974588158244185	29.829368581041344	20.5774923177284
4	21.542139902882322	27.001190385662788	30.11962097779331	21.33704873366158
5	24.069511652055933	27.290508957359933	28.5894154086625	20.050563981921634
6	22.05223553307607	26.961415801005543	30.172379212748723	20.81396945316966
7	23.544941654229856	27.568414520625257	28.530714949418982	20.3559288757259
8	21.691363424198332	27.20154985273776	30.25803995900651	20.849046764057398
9	21.800324927601856	27.089529732650753	30.327418310172177	20.782727029575216
10	21.883155065511247	27.897517971854807	29.52457804202615	20.694748920607793
11	23.408322761279337	27.22443835043624	28.509281372419405	20.857957515865024
12	21.547183161908883	28.99863824843211	28.507484773590143	20.94669381606887
13	22.708653723236875	27.53502855232507	28.727203784193396	21.029113940244663
14	22.812689329499147	27.67730539450434	28.36375771755204	21.14624755844447
15	22.42132983302456	28.059855180840472	28.218192249864128	21.300622736270842
16	22.571390338472476	27.673489761390073	28.351858674329357	21.40326122580809
17	22.49616544662921	27.730979745059287	28.39557616033141	21.37727864798009
18	22.407091471528688	27.61700500362271	28.404732523423686	21.57117100142491
19	22.27984511114281	27.778468488570766	28.503752022871183	21.437934377415242
20	22.13874758893827	27.785477159216587	28.529122615878666	21.546652635966478
21	22.219070393952336	27.785480089101704	28.41143615848295	21.584013358463007
22	22.14950180571483	27.84731787462399	28.477008768631062	21.52617155103012
23	22.17909939647161	27.906190681770752	28.361604941950535	21.553104979807102
24	22.10695777014676	27.91067681797226	28.31985134761489	21.662514064266094
25	22.11041697402792	28.033345028558255	28.245173240168263	21.61106475724556
26	22.16808577023853	27.973912235443972	28.225188897806436	21.632813096511068
27	22.153898552325774	27.92822341143725	28.15497832819227	21.762899708044703
28	22.158127453861137	27.94167318781819	28.172961267880442	21.72723809044023
29	22.16182839173238	28.010058719692637	28.12866408884996	21.699448799725026
30	22.211086269561296	27.942996937279656	28.043151083715124	21.802765709443925
31	22.296312053467055	27.909621496505338	28.006699993082822	21.787366456944785
32	22.307176730888923	27.97863084331859	27.937686969894294	21.776505455898192
33	22.2669822460326	27.92551111773141	27.90882786367721	21.898678772558778
34	22.327279190931208	27.957085825398266	27.847730020830397	21.86790496284013
35	22.294511682894147	27.96559251548942	27.869675652033337	21.870220149583098
36	22.33240966597722	27.925224598445205	27.83188675586966	21.910478979707914
37	22.341994017394367	27.9344800638827	27.85259588926219	21.870930029460748
38	22.303670549231935	27.950697603312864	27.903524874289143	21.842106973166057
39	22.30865675983949	27.835361080890504	27.907323505891306	21.948658653378697
40	22.35394672982975	27.84261043227547	27.89975133378783	21.90369150410695
41	22.313023167858923	27.920390232270748	27.873017694624778	21.89356890524555
42	22.28642210434692	27.857644494371993	27.84648519160523	22.00944820967586
43	22.32731441815986	27.892325077811904	27.799274917000183	21.98108558702805
44	22.377526649570775	27.90442664603754	27.77434909465533	21.94369760973635
45	22.378659952444785	27.844950142815435	27.762431857251425	22.01395804748835
46	22.28660728286853	27.92929674514564	27.75758653015639	22.026509441829436
47	22.30168452836777	27.88575421876054	27.795075169920924	22.01748608295077
48	22.34097274201156	27.830421925975585	27.733733047508522	22.094872284504333
49	22.366177891922746	27.92899368670777	27.68896857437421	22.015859846995273
50	22.310206356661812	27.89390043597107	27.762966483522682	22.032926723844433
51	22.32489903698882	27.82555331036117	27.780167919493078	22.069379733156936
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.83758034336345
2	41.07333215317994
3	42.196043260714475
4	42.8791886365439
5	44.12007563397757
6	42.866204986245734
7	43.90087052995576
8	42.54041018825573
9	42.58305195717707
10	42.57790398611904
11	44.26628027714436
12	42.49387697797775
13	43.737767663481534
14	43.958936887943615
15	43.721952569295404
16	43.97465156428057
17	43.873444094609304
18	43.9782624729536
19	43.717779488558044
20	43.68540022490475
21	43.80308375241535
22	43.67567335674495
23	43.73220437627871
24	43.769471834412855
25	43.721481731273485
26	43.80089886674959
27	43.91679826037048
28	43.88536554430137
29	43.861277191457404
30	44.01385197900522
31	44.083678510411836
32	44.083682186787115
33	44.16566101859138
34	44.195184153771336
35	44.164731832477244
36	44.242888645685134
37	44.212924046855115
38	44.14577752239799
39	44.25731541321819
40	44.2576382339367
41	44.206592073104474
42	44.29587031402278
43	44.30840000518791
44	44.32122425930713
45	44.39261799993314
46	44.31311672469797
47	44.31917061131854
48	44.43584502651589
49	44.382037738918015
50	44.34313308050625
51	44.39427877014575
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12274.0
1	8536.5
2	4799.0
3	7049.5
4	9300.0
5	15912.0
6	22524.0
7	28499.5
8	34475.0
9	29463.0
10	24451.0
11	28268.5
12	32086.0
13	42967.5
14	53849.0
15	78172.0
16	102495.0
17	158793.5
18	215092.0
19	271421.0
20	327750.0
21	385904.0
22	444058.0
23	522075.0
24	600092.0
25	715830.5
26	989819.5
27	1148070.0
28	1241177.5
29	1334285.0
30	1409374.0
31	1484463.0
32	1598527.5
33	1712592.0
34	1822109.5
35	1931627.0
36	2142266.0
37	2352905.0
38	2448959.0
39	2545013.0
40	2652423.5
41	2759834.0
42	2759581.0
43	2759328.0
44	2753599.5
45	2747871.0
46	2697798.0
47	2647725.0
48	2579139.5
49	2510554.0
50	2400826.5
51	2291099.0
52	2145108.5
53	1999118.0
54	1852135.5
55	1705153.0
56	1557210.0
57	1409267.0
58	1257496.0
59	1105725.0
60	976318.5
61	846912.0
62	729499.0
63	612086.0
64	526685.0
65	441284.0
66	370458.0
67	299632.0
68	274279.5
69	248927.0
70	218470.0
71	188013.0
72	160212.0
73	132411.0
74	110269.0
75	74283.5
76	60440.0
77	57028.5
78	53617.0
79	52682.0
80	51747.0
81	46104.5
82	40462.0
83	35665.0
84	30868.0
85	27953.0
86	25038.0
87	29927.0
88	34816.0
89	24275.5
90	13735.0
91	9110.0
92	4485.0
93	3406.5
94	2328.0
95	1814.0
96	1300.0
97	992.5
98	685.0
99	418.5
100	152.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003339406574312283
2	0.006862269782996584
3	0.005166535486909278
4	0.010414882716173647
5	0.013456792045558332
6	0.0016610262841790866
7	0.02972741219939019
8	0.003274948837911304
9	0.006445773640097949
10	9.470328963528524E-4
11	0.017135841307829622
12	0.01875720129268503
13	0.007040768129953143
14	0.016575554829882648
15	0.011969305820920343
16	0.030453801305755073
17	0.004844246804904381
18	0.0018172123377660754
19	0.038984534804053936
20	0.005203722642525228
21	3.2476782571262734E-4
22	0.02571615768028308
23	1.1404061055557908E-4
24	0.044664253038463324
25	0.016506138806066207
26	0.05942507467428763
27	0.07436191551336076
28	0.0027171415036720582
29	0.06744014628137865
30	0.016548284249097614
31	0.005300409247126697
32	0.001990752397307174
33	0.006569730825484447
34	0.025646741656466642
35	0.014250118032031927
36	0.01122556270860135
37	0.01882165902908601
38	0.041158743835733126
39	0.024560876712480913
40	0.03316598452201167
41	0.023170077092444393
42	0.02828207141778361
43	0.02980178651062209
44	0.012849401837164485
45	0.03603683293556299
46	0.05183889492863388
47	0.029422477523339402
48	0.05876066416061599
49	0.014200535157877326
50	0.05715169989429923
51	0.11406540199265637
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.0336508E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	36.598290827620445
#Duplication Level	Relative count
1	100.0
2	15.001022732883332
3	5.507945576503946
4	2.97862135607327
5	1.7873138802485593
6	1.2971074292727311
7	0.9112902656989498
8	0.731430344837315
9	0.5692037495503502
10++	12.282669262292192
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11391775	3.273765	3.8806098	16
TGTGT	5798815	2.7050114	18.050505	4
TGTTT	6085240	2.2280285	14.454892	6
GTTTG	4350960	2.0296211	17.499365	7
GAGGG	2585460	1.9932886	5.8576584	11
CTTTG	4043395	1.9521725	18.324532	1
TTTGA	5043925	1.8803573	14.281598	8
GTGTT	3826375	1.7849144	17.412888	5
TTTGT	4808075	1.7604115	14.1223955	2
TTGTG	3611080	1.6844844	17.397736	3
TGAGG	2761770	1.6712177	6.8447704	10
TTGAG	3202600	1.5211127	10.701814	9
AACTT	3502500	1.376008	5.6872883	2
ACTTT	3435900	1.3257283	5.319982	3
TAACT	2395070	0.94093806	5.351978	1
>>END_MODULE