FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_2_ATCACG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_2_ATCACG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 40336508
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11331025 3.1975772 3.7580895 16
TGTGT 5210550 2.4596438 18.5864 4
TGTTT 5575180 2.0348346 14.628735 6
GAGGG 2405140 1.9332016 6.0914125 11
CTTTG 3825575 1.8437835 18.543947 1
GTTTG 3798710 1.7931838 17.964563 7
TTTGT 4614370 1.6841574 14.35534 2
TTTGA 4532995 1.6840959 14.435703 8
TGAGG 2613100 1.6239564 7.2309923 10
GTGTT 3271880 1.5444933 17.890717 5
TTGTG 3126605 1.4759161 17.907974 3
TTGAG 2881810 1.3847307 10.966075 9
Produced by FastQC (version 0.10.1)