##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_2_ATCACG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	40336508	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.736067584234114	34.0	31.0	34.0	31.0	34.0
2	32.97289450539447	34.0	31.0	34.0	31.0	34.0
3	33.03828739959344	34.0	31.0	34.0	31.0	34.0
4	36.427243230871646	37.0	37.0	37.0	35.0	37.0
5	36.345992692277676	37.0	37.0	37.0	35.0	37.0
6	36.321948270782386	37.0	37.0	37.0	35.0	37.0
7	36.299830912482555	37.0	37.0	37.0	35.0	37.0
8	36.26836795093914	37.0	37.0	37.0	35.0	37.0
9	38.03891561460898	39.0	38.0	39.0	35.0	39.0
10	38.027394562761856	39.0	38.0	39.0	35.0	39.0
11	38.00961521012181	39.0	38.0	39.0	35.0	39.0
12	37.97753509054378	39.0	38.0	39.0	35.0	39.0
13	37.98581912445172	39.0	38.0	39.0	35.0	39.0
14	39.45386144978142	41.0	39.0	41.0	37.0	41.0
15	39.41769438742689	41.0	39.0	41.0	37.0	41.0
16	39.44013874478178	41.0	39.0	41.0	37.0	41.0
17	39.43502531255309	41.0	39.0	41.0	37.0	41.0
18	39.36495551374948	41.0	39.0	41.0	36.0	41.0
19	39.410067425767245	41.0	39.0	41.0	36.0	41.0
20	39.36694224993398	41.0	39.0	41.0	36.0	41.0
21	39.35329803462412	41.0	39.0	41.0	36.0	41.0
22	39.30645823381637	40.0	39.0	41.0	36.0	41.0
23	39.28465664405059	40.0	39.0	41.0	36.0	41.0
24	39.25147452030305	40.0	39.0	41.0	36.0	41.0
25	39.148410368096314	40.0	39.0	41.0	36.0	41.0
26	39.08322914318711	40.0	39.0	41.0	36.0	41.0
27	39.05791381346149	40.0	39.0	41.0	36.0	41.0
28	39.01428663581885	40.0	39.0	41.0	36.0	41.0
29	38.9653581316459	40.0	39.0	41.0	35.0	41.0
30	38.89589009539448	40.0	38.0	41.0	35.0	41.0
31	38.80655677977875	40.0	38.0	41.0	35.0	41.0
32	38.722535897257146	40.0	38.0	41.0	35.0	41.0
33	38.644544639312855	40.0	38.0	41.0	35.0	41.0
34	38.683886220393696	40.0	38.0	41.0	35.0	41.0
35	38.77169754010436	40.0	38.0	41.0	35.0	41.0
36	38.77044698564387	40.0	38.0	41.0	35.0	41.0
37	38.75329731071415	40.0	38.0	41.0	35.0	41.0
38	38.723366162484865	40.0	38.0	41.0	35.0	41.0
39	38.67702142188411	40.0	38.0	41.0	35.0	41.0
40	38.60854186981184	40.0	38.0	41.0	35.0	41.0
41	38.52895416727695	40.0	38.0	41.0	35.0	41.0
42	38.36864700831316	40.0	38.0	41.0	34.0	41.0
43	38.40271785549706	40.0	38.0	41.0	34.0	41.0
44	38.06949535641509	40.0	38.0	41.0	33.0	41.0
45	38.19829066512153	40.0	38.0	41.0	34.0	41.0
46	38.21162667328565	40.0	38.0	41.0	34.0	41.0
47	38.1595711259884	40.0	38.0	41.0	34.0	41.0
48	38.10887980189064	40.0	38.0	41.0	34.0	41.0
49	37.87051516705412	40.0	37.0	41.0	33.0	41.0
50	37.76257334918531	40.0	37.0	41.0	33.0	41.0
51	37.68044412768701	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	11.0
10	12.0
11	54.0
12	88.0
13	115.0
14	204.0
15	323.0
16	586.0
17	969.0
18	1764.0
19	3311.0
20	6566.0
21	11758.0
22	20101.0
23	32035.0
24	48021.0
25	69009.0
26	96837.0
27	130617.0
28	175329.0
29	231190.0
30	302822.0
31	393263.0
32	513709.0
33	685351.0
34	970784.0
35	1431743.0
36	2189595.0
37	3708258.0
38	8220460.0
39	2.1080296E7
40	11327.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.907477837123015	29.28387229754943	28.14360276617622	23.665047099151337
2	21.579798122136378	28.793962589939614	30.539407253042533	19.086832034881475
3	19.85520989520115	27.572755769490126	31.49546364170058	21.076570693608144
4	20.64776351009552	27.522775878923323	30.925472027948615	20.903988583032543
5	23.621754763699425	27.655710801738216	28.706213239876888	20.016321194685467
6	22.15997478016689	27.414916531693816	29.62685813060466	20.79825055753463
7	23.53288489920843	28.129494997435078	27.89086254070382	20.446757562652675
8	21.31360999321012	27.567450806599318	30.078910152559562	21.040029047630995
9	21.115318162891047	27.41041440672058	30.492418927290384	20.98184850309799
10	21.452328000232445	27.69862973760644	29.940930930362143	20.908111331798978
11	23.118533316511012	27.610496820142732	28.306433116738116	20.96453674660814
12	21.400781644261325	29.3793404228249	28.167301938977964	21.052575993935815
13	22.42610263401778	27.86527612623989	28.547093276928813	21.161527962813516
14	22.212118609771576	27.956901970790334	28.59044714530073	21.240532274137365
15	22.214173819905284	27.909503222242243	28.502826769238425	21.373496188614048
16	22.152572956060272	27.855346722900315	28.53368972425808	21.458390596781342
17	22.19052201328437	27.865751422306932	28.480908808670762	21.462817755737934
18	22.151414854326735	27.834369515739382	28.462795063571683	21.5514205663622
19	21.959469074516814	27.94455087683842	28.60896882794118	21.487011220703586
20	21.8920190116606	27.985439394010015	28.585560753052793	21.53698084127659
21	21.984885255808457	27.912421174386242	28.48410427595765	21.61858929384765
22	21.955455340853998	27.963704741124346	28.508811422148888	21.57202849587277
23	21.99686695734792	27.979023370094406	28.415074998559618	21.609034673998057
24	21.931160996641022	27.984113950281397	28.377234031523436	21.707491021554144
25	21.975164533330453	28.03898641895327	28.284957141059408	21.700891906656867
26	22.037827369687037	28.02577258299107	28.267697342566194	21.668702704755702
27	22.03553664090109	27.95711021886178	28.203839558942484	21.80351358129464
28	22.05323936716877	27.99876191554028	28.217162096716113	21.730836620574838
29	22.065055805029033	28.056501569248383	28.19223220810289	21.68621041761969
30	22.093751893446008	27.985697224955615	28.145039228482542	21.775511653115835
31	22.18350929138437	27.958446477320248	28.07312918609613	21.784915045199252
32	22.196755356214773	28.041661415013913	27.997931799153264	21.763651429618054
33	22.145494067530418	27.970420827394747	27.99565604245684	21.88842906261799
34	22.154579000006123	28.06511513312312	27.90597562637551	21.874330240495244
35	22.166665695668403	28.006414538496717	27.961408149121535	21.865511616713352
36	22.222787743303222	27.98811209128062	27.892201313615946	21.89689885180021
37	22.21425563164764	27.997792967105628	27.914587945986796	21.873363455259934
38	22.183425000498307	28.01982015894881	27.97700782626002	21.819747014292858
39	22.19457123060826	27.880122890161935	27.977741652797512	21.94756422643229
40	22.215523280036127	27.95549725142697	27.96000682758259	21.868972640954315
41	22.22065804674852	27.95743027302843	27.94914934405001	21.87276233617304
42	22.1562071890448	27.827174803342007	27.92789931674032	22.088718690872877
43	22.204651116436377	27.960634982432627	27.870986477903603	21.96372742322739
44	22.39389549600223	27.970077242188786	27.755926047974828	21.88010121383416
45	22.280080294179278	27.835504727874156	27.884669640841025	21.999745337105544
46	22.19429956690037	27.940011151652307	27.873773113708427	21.9919161677389
47	22.229461603118764	27.98914378815417	27.845453964388728	21.93594064433834
48	22.239598498594514	27.89712438879452	27.82310634400148	22.040170768609485
49	22.323985011295473	27.979727446907404	27.686119978457235	22.01016756333989
50	22.21934234682812	27.77898270794482	27.78723221611718	22.21444272910988
51	22.238016230253418	27.86045651563113	27.841970084031896	22.059557170083554
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.572524936274355
2	40.66663015701785
3	40.93178058880929
4	41.55175209312806
5	43.638075958384896
6	42.95822533770151
7	43.979642461861104
8	42.35363904084112
9	42.097166665989036
10	42.36043933203142
11	44.08307006311915
12	42.45335763819714
13	43.587630596831296
14	43.45265088390894
15	43.58767000851933
16	43.61096355284161
17	43.6533397690223
18	43.702835420688935
19	43.446480295220404
20	43.428999852937196
21	43.603474549656106
22	43.52748383672677
23	43.60590163134598
24	43.63865201819517
25	43.67605643998732
26	43.70653007444273
27	43.83905022219574
28	43.784075987743606
29	43.751266222648724
30	43.86926354656184
31	43.968424336583624
32	43.960406785832824
33	44.03392313014841
34	44.02890924050137
35	44.032177312381755
36	44.11968659510343
37	44.08761908690757
38	44.00317201479116
39	44.14213545704055
40	44.08449592099044
41	44.09342038292156
42	44.24492587991768
43	44.16837853966376
44	44.27399670983639
45	44.27982563128482
46	44.18621573463927
47	44.16540224745711
48	44.279769267204
49	44.33415257463537
50	44.433785075938
51	44.29757340033697
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19730.0
1	12486.5
2	5243.0
3	7816.0
4	10389.0
5	12650.5
6	14912.0
7	23117.5
8	31323.0
9	27208.0
10	23093.0
11	27442.0
12	31791.0
13	43207.0
14	54623.0
15	81583.0
16	108543.0
17	168752.5
18	228962.0
19	290906.0
20	352850.0
21	412233.5
22	471617.0
23	550672.0
24	629727.0
25	753711.0
26	1039186.5
27	1200678.0
28	1286510.0
29	1372342.0
30	1442846.0
31	1513350.0
32	1619930.0
33	1726510.0
34	1836126.0
35	1945742.0
36	2146976.5
37	2348211.0
38	2439405.0
39	2530599.0
40	2628863.5
41	2727128.0
42	2725726.0
43	2724324.0
44	2719561.5
45	2714799.0
46	2664486.0
47	2614173.0
48	2550186.0
49	2486199.0
50	2377735.5
51	2269272.0
52	2129688.5
53	1990105.0
54	1843883.5
55	1697662.0
56	1550458.0
57	1403254.0
58	1250584.5
59	1097915.0
60	969748.0
61	841581.0
62	723566.0
63	605551.0
64	521992.0
65	438433.0
66	366126.0
67	293819.0
68	270140.0
69	246461.0
70	216246.5
71	186032.0
72	157548.5
73	129065.0
74	107036.0
75	70608.5
76	56210.0
77	53732.0
78	51254.0
79	50847.5
80	50441.0
81	44222.5
82	38004.0
83	32637.0
84	27270.0
85	24727.5
86	22185.0
87	26945.5
88	31706.0
89	20293.5
90	8881.0
91	5066.5
92	1252.0
93	851.5
94	451.0
95	275.0
96	99.0
97	65.5
98	32.0
99	22.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02607067523048847
2	3.446009753744672E-4
3	4.6607901705323626E-4
4	0.03313623479751891
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.002992326455230086
12	0.0
13	0.002992326455230086
14	0.0
15	0.0
16	6.867228070412044E-4
17	0.007660554056885638
18	0.0015469856736235077
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0018370454874279151
25	0.0
26	0.0
27	0.0
28	7.43743112318994E-6
29	0.0
30	0.0
31	0.0
32	0.0
33	4.462458673913964E-5
34	4.98307885253726E-4
35	3.2228868200489736E-5
36	1.512277661715288E-4
37	0.0
38	0.0
39	0.0
40	3.0493467605078754E-4
41	0.007112663297477313
42	0.001849441205966565
43	2.107272151570483E-4
44	0.0012891547280195895
45	0.002062647564831343
46	0.004160003141570907
47	0.0076903037813783985
48	0.007685345493962938
49	0.0
50	0.016935030667503494
51	0.06411561456931274
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.0336508E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	37.915938506302844
#Duplication Level	Relative count
1	100.0
2	15.400332926106122
3	5.860499674932664
4	3.1299340577691095
5	1.894678183337977
6	1.3552807367240356
7	1.0173535946731822
8	0.7208636074615457
9	0.5929799744232734
10++	12.914818069457244
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11331025	3.1975772	3.7580895	16
TGTGT	5210550	2.4596438	18.5864	4
TGTTT	5575180	2.0348346	14.628735	6
GAGGG	2405140	1.9332016	6.0914125	11
CTTTG	3825575	1.8437835	18.543947	1
GTTTG	3798710	1.7931838	17.964563	7
TTTGT	4614370	1.6841574	14.35534	2
TTTGA	4532995	1.6840959	14.435703	8
TGAGG	2613100	1.6239564	7.2309923	10
GTGTT	3271880	1.5444933	17.890717	5
TTGTG	3126605	1.4759161	17.907974	3
TTGAG	2881810	1.3847307	10.966075	9
>>END_MODULE