FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_17_TGACCA_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_17_TGACCA_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33021066
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 8780345 3.019371 3.5683928 16
TGTGT 5310660 3.0037286 20.811852 4
TGTTT 5265120 2.3220246 16.398996 6
GTTTG 3770435 2.132572 20.153826 7
CTTTG 3427640 2.0181751 21.11576 1
GAGGG 2118400 2.0067277 6.4046197 11
TTTGA 4314450 1.9375145 16.259836 8
GTGTT 3353045 1.8964947 20.043062 5
TTTGT 4146155 1.8285383 16.042376 2
TTGTG 3157355 1.7858115 20.033112 3
TGAGG 2334835 1.7245787 7.825873 10
TTGAG 2802500 1.6140568 12.691837 9
AACTT 2872510 1.3673933 5.879932 2
ACTTT 2834880 1.3252738 5.4622545 3
TAACT 1987485 0.94609714 5.5824256 1