##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_17_TGACCA_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	33021066	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.337873919636635	34.0	31.0	34.0	31.0	34.0
2	32.49143107614999	34.0	31.0	34.0	31.0	34.0
3	32.547413036272054	34.0	31.0	34.0	31.0	34.0
4	35.99583717254919	37.0	37.0	37.0	35.0	37.0
5	36.001216587011456	37.0	37.0	37.0	35.0	37.0
6	36.00978766100404	37.0	37.0	37.0	35.0	37.0
7	36.00211186398404	37.0	37.0	37.0	35.0	37.0
8	36.019420299756526	37.0	37.0	37.0	35.0	37.0
9	37.768977052406484	39.0	38.0	39.0	35.0	39.0
10	37.7550927035487	39.0	38.0	39.0	35.0	39.0
11	37.7208450205696	39.0	38.0	39.0	35.0	39.0
12	37.68622596859835	39.0	38.0	39.0	35.0	39.0
13	37.68466030139669	39.0	38.0	39.0	35.0	39.0
14	39.190450968481755	41.0	39.0	41.0	36.0	41.0
15	39.15940481751861	41.0	39.0	41.0	36.0	41.0
16	39.11152429179603	41.0	39.0	41.0	36.0	41.0
17	39.09995279982785	41.0	39.0	41.0	36.0	41.0
18	39.07030693678999	41.0	39.0	41.0	36.0	41.0
19	39.080747332627	41.0	39.0	41.0	36.0	41.0
20	39.04403131625127	41.0	39.0	41.0	36.0	41.0
21	39.01455467851947	41.0	39.0	41.0	36.0	41.0
22	38.965860672093385	41.0	39.0	41.0	36.0	41.0
23	38.93153231334203	41.0	39.0	41.0	36.0	41.0
24	38.8657419781663	41.0	39.0	41.0	35.0	41.0
25	38.83045847157084	41.0	39.0	41.0	35.0	41.0
26	38.790023193073175	40.0	39.0	41.0	35.0	41.0
27	38.73583299824421	40.0	39.0	41.0	35.0	41.0
28	38.68335767839839	40.0	39.0	41.0	35.0	41.0
29	38.578193326647906	40.0	39.0	41.0	35.0	41.0
30	38.561371337921074	40.0	38.0	41.0	35.0	41.0
31	38.51713821110439	40.0	38.0	41.0	34.0	41.0
32	38.460808775828134	40.0	38.0	41.0	34.0	41.0
33	38.406895343717856	40.0	38.0	41.0	34.0	41.0
34	38.33514572182497	40.0	38.0	41.0	34.0	41.0
35	38.2919102005974	40.0	38.0	41.0	34.0	41.0
36	38.240763305460824	40.0	38.0	41.0	34.0	41.0
37	38.17787333092154	40.0	38.0	41.0	34.0	41.0
38	38.10475479501479	40.0	38.0	41.0	33.0	41.0
39	38.05063237510261	40.0	38.0	41.0	33.0	41.0
40	37.970536656811746	40.0	38.0	41.0	33.0	41.0
41	38.16300345967026	40.0	38.0	41.0	34.0	41.0
42	38.19907631086168	40.0	38.0	41.0	34.0	41.0
43	38.18085021846357	40.0	38.0	41.0	34.0	41.0
44	38.1346602196307	40.0	38.0	41.0	33.0	41.0
45	38.098376169927405	40.0	38.0	41.0	33.0	41.0
46	38.032785252904915	40.0	38.0	41.0	33.0	41.0
47	37.98412773833528	40.0	38.0	41.0	33.0	41.0
48	37.915099470138244	40.0	38.0	41.0	33.0	41.0
49	37.85289914626015	40.0	38.0	41.0	33.0	41.0
50	37.749621529480606	40.0	38.0	41.0	33.0	41.0
51	36.26969656279419	38.0	35.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	25.0
5	79.0
6	485.0
7	1908.0
8	6323.0
9	10731.0
10	14486.0
11	18404.0
12	25478.0
13	33881.0
14	41748.0
15	46887.0
16	49258.0
17	51213.0
18	52742.0
19	53724.0
20	56379.0
21	60362.0
22	65579.0
23	72673.0
24	82142.0
25	94351.0
26	108480.0
27	127550.0
28	151013.0
29	182586.0
30	226363.0
31	282784.0
32	368721.0
33	496171.0
34	707824.0
35	1045105.0
36	1600001.0
37	2711841.0
38	6370759.0
39	1.7799291E7
40	3717.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.01103081587305	28.890125908598495	28.478429718660593	22.620413556867863
2	22.31080890207298	29.51319226278748	29.66017776235829	18.515821072781247
3	21.877631466339732	28.12413710314012	29.8834518769519	20.114779553568244
4	21.640759699209994	27.080561163197466	30.361736537190026	20.916942600402514
5	24.228816079652038	27.397544247935013	28.498074780993843	19.8755648914191
6	21.957259041849227	27.015436146125626	30.38336497071294	20.64393984131221
7	23.673595887574926	27.537566388281796	28.579593818301458	20.209243905841817
8	21.668507679940184	27.377372995446958	30.310109411456697	20.64400991315616
9	21.76175252595718	27.217272490473082	30.447162115380323	20.57381286818941
10	21.758547554125094	27.96482416701931	29.84871597143581	20.427912307419792
11	23.60820207130936	27.31113661552878	28.53502661937362	20.545634693788237
12	21.365354838785894	29.33046340598792	28.622724152895813	20.68145760233037
13	22.69983266730294	27.51550213393334	28.91098419654485	20.873681002218877
14	22.75964660579057	27.654775327144286	28.555741820014823	21.029836247050312
15	22.421349593053723	28.10956643417468	28.300618067965367	21.16846590480623
16	22.532140649453847	27.72815802608943	28.440557909524493	21.299143414932225
17	22.483197705047964	27.753840227108668	28.47875989727594	21.28420217056743
18	22.3936722490411	27.708597843880366	28.460082623441913	21.43764728363662
19	22.22309330987617	27.85428219538202	28.61257274940881	21.310051745333
20	22.081428767976114	27.9113013957813	28.604847710331686	21.4024221259109
21	22.175478614380477	27.894308716572098	28.523254771607302	21.406957897440122
22	22.086728283874898	27.963697884263684	28.597606084496114	21.351967747365304
23	22.10968957256212	27.993347297716753	28.49006184756225	21.406901282158874
24	22.02846754294747	28.036489874126307	28.447296322361186	21.487746260565032
25	22.043370882662757	28.151987763202047	28.36983553326459	21.434805820870604
26	22.08582935848415	28.09940242009049	28.35623812975064	21.45853009167472
27	22.073500019212382	28.055710810169685	28.291917018519886	21.578872152098047
28	22.065710864093262	28.07731667620042	28.312674809292826	21.544297650413487
29	22.089815628611245	28.158543231344257	28.239721909373433	21.51191923067106
30	22.10425495400918	28.090103446930154	28.17417513574728	21.63146646331339
31	22.20506468739321	28.06274845411796	28.119136385882836	21.61305047260599
32	22.20533868388012	28.11313311678378	28.060828480477305	21.620699718858795
33	22.209787112147318	28.047132298131622	28.01962582656856	21.7234547631525
34	22.210658950566046	28.08826804837799	28.01366656721822	21.68740643383774
35	22.191092371550774	28.076024300586145	27.999959658330287	21.73292366953279
36	22.21714984147237	28.086606447448553	27.968015107584478	21.7282286034946
37	22.22161310374965	28.03727066033275	27.99414709165179	21.74696914426581
38	22.181626306662306	28.081839959896275	28.043442066765916	21.693091666675503
39	22.165844463819692	28.011392007784885	28.033902457182748	21.78886107121268
40	22.219817810853428	27.996915486586417	28.005067864990558	21.7781988375696
41	22.203316654931793	28.018712028881904	27.985288388618283	21.79268292756802
42	22.171002201416577	27.969567208207046	27.999629618983402	21.85980097139297
43	22.22580896205717	28.057236006784393	27.89495723464055	21.821997796517888
44	22.250707202868792	28.03141676812832	27.88425193988624	21.83362408911665
45	22.24636086814183	27.99692548071323	27.893683164262338	21.863030486882597
46	22.173745204272606	28.079761473764624	27.870287924344268	21.876205397618502
47	22.2057659354986	28.051846815444964	27.887766293450607	21.85462095560583
48	22.206063761761712	28.022130808444096	27.854809233254187	21.916996196540005
49	22.256215564402478	28.08231192792585	27.80750857600154	21.85396393167013
50	22.169039229128394	28.066810578106114	27.895488734954547	21.86866145781094
51	22.234661339867227	27.995944133375534	27.86611977135824	21.903274755399003
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.631444372740916
2	40.82662997485423
3	41.992411019907976
4	42.55770229961251
5	44.10438097107114
6	42.60119888316144
7	43.88283979341674
8	42.312517593096345
9	42.3355653941466
10	42.18645986154488
11	44.1538367650976
12	42.04681244111626
13	43.573513669521816
14	43.78948285284089
15	43.58981549785995
16	43.831284064386075
17	43.76739987561539
18	43.83131953267772
19	43.53314505520917
20	43.48385089388701
21	43.582436511820596
22	43.438696031240205
23	43.516590854720995
24	43.51621380351251
25	43.478176703533364
26	43.54435945015887
27	43.652372171310425
28	43.610008514506745
29	43.60173485928231
30	43.735721417322566
31	43.8181151599992
32	43.82603840273892
33	43.933241875299814
34	43.898065384403786
35	43.92401604108357
36	43.94537844496696
37	43.968582248015466
38	43.87471797333781
39	43.954705535032375
40	43.99801664842303
41	43.99599958249981
42	44.03080317280955
43	44.04780675857506
44	44.08433129198544
45	44.10939135502443
46	44.049950601891105
47	44.06038689110443
48	44.12305995830172
49	44.11017949607261
50	44.03770068693933
51	44.13793609526623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6610.0
1	4831.0
2	3052.0
3	4108.0
4	5164.0
5	8906.5
6	12649.0
7	16099.0
8	19549.0
9	18392.0
10	17235.0
11	21189.0
12	25143.0
13	34081.0
14	43019.0
15	63019.0
16	83019.0
17	129179.0
18	175339.0
19	220501.5
20	265664.0
21	311674.0
22	357684.0
23	424652.5
24	491621.0
25	589077.5
26	814065.5
27	941597.0
28	1021099.5
29	1100602.0
30	1167026.5
31	1233451.0
32	1331037.5
33	1428624.0
34	1523268.5
35	1617913.0
36	1787031.0
37	1956149.0
38	2034701.5
39	2113254.0
40	2197363.5
41	2281473.0
42	2289145.0
43	2296817.0
44	2301851.5
45	2306886.0
46	2260848.0
47	2214810.0
48	2155356.5
49	2095903.0
50	1996729.5
51	1897556.0
52	1772468.0
53	1647380.0
54	1515994.5
55	1384609.0
56	1255648.5
57	1126688.0
58	995242.0
59	863796.0
60	758019.0
61	652242.0
62	557473.5
63	462705.0
64	393519.5
65	324334.0
66	270411.5
67	216489.0
68	196496.5
69	176504.0
70	153546.0
71	130588.0
72	110820.5
73	91053.0
74	75697.0
75	50878.5
76	41416.0
77	38522.5
78	35629.0
79	35057.0
80	34485.0
81	30231.0
82	25977.0
83	22833.0
84	19689.0
85	17500.0
86	15311.0
87	18467.5
88	21624.0
89	14778.5
90	7933.0
91	5221.0
92	2509.0
93	1883.0
94	1257.0
95	981.5
96	706.0
97	542.5
98	379.0
99	242.0
100	105.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0025529157659537702
2	0.0024287526029595774
3	8.479435521554635E-5
4	4.6939732351463157E-4
5	0.004379022772917143
6	0.0
7	0.01992667347565339
8	9.115393185671231E-4
9	0.003655242383755873
10	0.003400859318109234
11	0.008543031287966293
12	0.023981660676853984
13	0.014551317028953578
14	0.00697130734664956
15	0.005208796106097847
16	0.04932608777681495
17	0.007604236640937031
18	5.17851240780658E-4
19	0.030201932305880134
20	0.003225213868019888
21	0.0022591638925284845
22	0.02257044033648096
23	0.006974335716478687
24	0.08453088704041233
25	0.03566511147762462
26	0.02698883191717675
27	0.06514023502451435
28	0.009990592066288835
29	0.09723489847359863
30	0.05175484037977454
31	0.026295335226306747
32	0.03212494714737556
33	0.02125007109098174
34	0.03736402695176467
35	0.00937886136080525
36	0.010532670265702506
37	0.013076500922168898
38	0.05042538602478793
39	0.016014019656421755
40	0.037230778679283094
41	0.016692374498146124
42	0.01942699245384749
43	0.032061351380963894
44	0.011102003793578319
45	0.0314253937168473
46	0.03238235858285132
47	0.03347560009116605
48	0.0567728491866374
49	0.0066079029671543615
50	0.09067847779353944
51	0.1292023703898596
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.3021066E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.34386955357588
#Duplication Level	Relative count
1	100.0
2	11.137781437030938
3	3.138161436397606
4	1.4819975300041166
5	0.8758985401690997
6	0.5832990278349536
7	0.4262326229456284
8	0.3609993983343361
9	0.2843661927230121
10++	8.377719370467716
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	8780345	3.019371	3.5683928	16
TGTGT	5310660	3.0037286	20.811852	4
TGTTT	5265120	2.3220246	16.398996	6
GTTTG	3770435	2.132572	20.153826	7
CTTTG	3427640	2.0181751	21.11576	1
GAGGG	2118400	2.0067277	6.4046197	11
TTTGA	4314450	1.9375145	16.259836	8
GTGTT	3353045	1.8964947	20.043062	5
TTTGT	4146155	1.8285383	16.042376	2
TTGTG	3157355	1.7858115	20.033112	3
TGAGG	2334835	1.7245787	7.825873	10
TTGAG	2802500	1.6140568	12.691837	9
AACTT	2872510	1.3673933	5.879932	2
ACTTT	2834880	1.3252738	5.4622545	3
TAACT	1987485	0.94609714	5.5824256	1
>>END_MODULE