FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_17_TGACCA_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_17_TGACCA_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33021066
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 4711495 2.7525601 21.658485 4
TGTTT 4730585 2.1165354 16.723188 6
GAGGG 1931585 1.9576579 6.941232 11
CTTTG 3197830 1.9159286 21.51634 1
GTTTG 3235765 1.8904061 20.860064 7
TTTGT 3923000 1.7552097 16.363066 2
TTTGA 3811360 1.7350051 16.54281 8
TGAGG 2162145 1.6781871 8.3422365 10
GTGTT 2821485 1.6483743 20.773304 5
TTGTG 2707005 1.5814927 20.772766 3
TTGAG 2483975 1.4765066 13.2313175 9
Produced by FastQC (version 0.10.1)